Heterocyclic compound

ABSTRACT

The present invention provides a heterocyclic compound having an orexin type 2 receptor agonist activity.A compound represented by the formula (I):wherein each symbol is as described in the description, or a salt thereof has an orexin type 2 receptor agonist activity, and is useful as an agent for the prophylaxis or treatment of narcolepsy.

TECHNICAL FIELD

The present invention relates to a heterocyclic compound, particularly, a heterocyclic compound having an orexin type 2 receptor agonist activity.

BACKGROUND OF THE INVENTION

Orexin is a neuropeptide specifically produced in particular neurons located sparsely in the lateral hypothalamus and its surrounding area, and consists of two subtypes, orexin A and orexin B. Both orexin A and orexin B are endogenous ligands of the orexin receptors, which are G protein-coupled receptors mainly present in the brain, and two types of subtypes, type 1 and type 2, are known for the orexin receptors (non-patent document 1).

Since orexin-producing neurons (orexin neurons) are localized in the vicinity of the feeding center, and intraventricular administration of orexin peptide results in an increase in food intake, orexin initially attracted attention as a neuropeptide having a feeding behavioral regulation. Thereafter, however, it was reported that the cause of dog narcolepsy is genetic variation of orexin type 2 receptor (non-patent document 2), and the role of orexin in controlling sleep and wakefulness has been also attracted.

From the studies using a transgenic mouse having denatured orexin neurons and a double transgenic mouse obtained by crossing this mouse with orexin overexpressing transgenic mouse, it was clarified that narcolepsy-like symptoms that appear by degeneration of orexin neurons disappear due to sustained expression of orexin. Similarly, when orexin peptide was intraventricularly administered to a transgenic mouse having denatured orexin neuron, improvement of narcolepsy-like symptoms was also observed (non-patent document 3). Studies of orexin type 2 receptor knockout mice have suggested that orexin type 2 receptor is important for maintaining arousal (non-patent document 4, non-patent document 5). Such background suggests that orexin type 2 receptor agonists become therapeutic drugs for narcolepsy or therapeutic drugs for other sleep disorders exhibiting excessive sleepiness (non-patent document 6).

In addition, it is suggested that a peptidic agonist that selectively acts on the orexin type 2 receptor improves obesity due to high fat diet load in mice (non-patent document 7).

In addition, it is suggested that intraventricular administration of orexin peptide shortens the systemic anesthetic time of rat (non-patent document 8).

In addition, it is suggested that patients with sleep apnea syndrome show low orexin A concentration levels in plasma (non-patent document 9).

In addition, it is suggested that intraventricular administration of orexin peptide improves memory retention of senescence-accelerated model mouse (SAMP8) with cognitive dysfunction (non-patent document 10).

In addition, it is suggested that Orexin type 2 receptor agonist will be a therapeutic drug for cardiac failure (patent document 1, non-patent document 11).

In addition, it is suggested that the daytime sleepiness of Parkinson's disease patients is caused by orexin nerve fallout (non-patent document 12).

In addition, it is suggested that orexin regulates bone formation and bone loss, and orexin type 2 receptor agonist will be a therapeutic drug for diseases related to bone loss such as osteoporosis, rheumatoid arthritis and the like (patent document 2).

In addition, it is suggested that orexin receptor agonist is useful for the prophylaxis or treatment of sepsis, severe sepsis and septic shock, since the mortality was significantly improved by mere continuous administration of orexin from the periphery in septic shock model mouse (patent document 3).

Therefore, a compound having an orexin type 2 receptor agonist activity is expected to be useful as a novel therapeutic drug for narcolepsy, idiopathic hypersomnia, hypersomnia, sleep apnea syndrome, disturbance of consciousness such as coma and the like, narcolepsy syndrome accompanied by narcolepsy-like symptoms, hypersomnia syndrome accompanied by daytime hypersomnia (e.g., Parkinson's disease, Guillain-Barre syndrome and Kleine Levin syndrome), Alzheimer, obesity, insulin resistance syndrome, cardiac failure, diseases related to bone loss, sepsis and the like, further, anesthetic antagonist, a prophylactic or therapeutic drug for side effects and complications due to anesthesia.

As sulfonamide derivatives, a compound represented by the formula

wherein each symbol is as described in the document (Patent Document 4) has been reported.

In addition, as compounds having an orexin type 2 receptor agonist activity, the following compounds have been reported.

A compound represented by the formula

wherein each symbol is as described in the document (Patent Document 5).

A compound represented by the formula

wherein each symbol is as described in the document (Patent Document 6).

A compound represented by the formula

wherein each symbol is as described in the document (Patent Document 7).

A compound represented by the formula

wherein each symbol is as described in the document (Patent Document 8).

A compound represented by the formula

wherein each symbol is as described in the document (Patent Document 9).

A compound represented by the formula

wherein each symbol is as described in the document (Patent Document 10).

A compound represented by the formula

wherein each symbol is as described in the document (Patent Document 11).

A compound represented by the formula

wherein each symbol is as described in the document (Patent Document 12).

A compound represented by the formula

wherein each symbol is as described in the document (Patent Document 13).

A compound represented by the formula

wherein each symbol is as described in the document (Patent Document 14).

A compound represented by the formula

wherein each symbol is as described in the document (Patent Document 15).

A compound represented by the formula

wherein each symbol is as described in the document (Patent Document 16).

However, it is considered that these compounds are not satisfactory in terms of activity, pharmacokinetics or safety, and therefore, development of a compound having an orexin type 2 receptor agonist activity is still desired.

DOCUMENT LIST Patent Document

-   Patent Document 1: WO 2015/073707 A1 -   Patent Document 2: WO 2015/048091 A1 -   Patent Document 3: WO 2015/147240 A1 -   Patent Document 4: WO 2012/137982 A9 -   Patent Document 5: WO 2017/135306 A1 -   Patent Document 6: WO 2018/164191 A1 -   Patent Document 7: WO 2018/164192 A1 -   Patent Document 8: WO 2019/027003 A1 -   Patent Document 9: WO 2019/027058 A1 -   Patent Document 10: WO 2020/004536 A1 -   Patent Document 11: WO 2020/004537 A1 -   Patent Document 12: WO 2020/122092 A1 -   Patent Document 13: WO 2020/122093 A1 -   Patent Document 14: WO 2020/158958 A1 -   Patent Document 15: WO 2020/167701 A1 -   Patent Document 16: WO 2020/167706 A1

Non-Patent Document

-   Non-Patent Document 1: Cell, Vol. 92, 573-585, 1998 -   Non-Patent Document 2: Cell, Vol. 98, 365-376, 1999 -   Non-Patent Document 3: Proc. Natl. Acad. Sci. USA, Vol. 101,     4649-4654, 2004 -   Non-Patent Document 4: Cell, Vol. 98, 437-451, 1999 -   Non-Patent Document 5: Neuron, Vol. 38, 715-730, 2003 -   Non-Patent Document 6: CNS Drugs, Vol. 27, 83-90, 2013 -   Non-Patent Document 7: Cell Metabolism, Vol. 9, 64-76, 2009 -   Non-Patent Document 8: Neuroscience, Vol. 121, 855-863, 2003 -   Non-Patent Document 9: Respiration, Vol. 71, 575-579, 2004 -   Non-Patent Document 10: Peptides, Vol. 23, 1683-1688, 2002 -   Non-Patent Document 11: Journal of the American College of     Cardiology. Vol. 66, 2015, Pages 2522-2533 -   Non-Patent Document 12: Brain. Vol. 130, 2007, Pages 1586-1595

SUMMARY OF THE INVENTION Problems to be Solved by the Invention

The present invention aims to provide a heterocyclic compound having an orexin type 2 receptor agonist activity.

Means of Solving the Problems

The present inventors have found that a compound represented by the following formula (I) or a salt thereof (sometimes to be referred to as compound (I) in the present description) has an orexin type 2 receptor agonist activity. As a result of further studies, they have completed the present invention.

Accordingly, the present invention relates to the followings.

[1] A compound represented by the formula:

wherein R¹ is an optionally substituted C₁₋₆ alkyl group, an optionally substituted mono- or di-C₁₋₆ alkylamino group, or an optionally substituted C₃₋₆ cycloalkyl group; R² is a hydrogen atom, a halogen atom, a cyano group, an optionally substituted C₁₋₆ alkyl group, an optionally substituted C₃₋₆ cycloalkyl group, or an optionally substituted C₁₋₆ alkoxy group; R³ is a hydrogen atom, a halogen atom, a cyano group, an optionally substituted C₁₋₆ alkyl group, an optionally substituted C₃₋₆ cycloalkyl group, or an optionally substituted C₁₋₆ alkoxy group; and R⁴ is an optionally substituted C₁₋₆ alkyl group, an optionally substituted C₃₋₆ cycloalkyl group, an optionally substituted C₂₋₆ alkenyl group, an optionally substituted C₂₋₆ alkynyl group, an optionally substituted C₆₋₁₀ aryl group, an optionally substituted C₁₋₆ alkoxy group, an optionally substituted C₃₋₆ cycloalkoxy group, or an optionally substituted 4- to 6-membered heterocyclic group, or a salt thereof. [2] The compound or salt according to the above-mentioned [1], wherein R² is a hydrogen atom, a halogen atom, an optionally substituted C₁₋₆ alkyl group, or an optionally substituted C₁₋₆ alkoxy group; R³ is a hydrogen atom, a halogen atom, an optionally substituted C₁₋₆ alkyl group, an optionally substituted C₃₋₆ cycloalkyl group, or an optionally substituted C₁₋₆ alkoxy group; and R⁴ is an optionally substituted C₁₋₆ alkyl group, an optionally substituted C₆₋₁₀ aryl group, or an optionally substituted C₁₋₆ alkoxy group. [3] The compound or salt according to the above-mentioned [1], wherein R¹ is a C₁₋₆ alkyl group, a mono- or di-C₁₋₆ alkylamino group, or a C₃₋₆ cycloalkyl group; R² is a hydrogen atom, a halogen atom, a cyano group, a C₁₋₆ alkyl group, a C₃₋₆ cycloalkyl group, or a C₁₋₆ alkoxy group; R³ is a hydrogen atom, a halogen atom, a cyano group, a C₁₋₆ alkyl group optionally substituted by 1 to 3 hydroxy groups, a C₃₋₆ cycloalkyl group, or a C₁₋₆ alkoxy group; and

R⁴ is

(1) a C₁₋₆ alkyl group optionally substituted by 1 to 3 substituents selected from a halogen atom, a hydroxy group, a C₃₋₆ cycloalkyl group, a C₁₋₆ alkoxy group, a C₁₋₆ alkoxy-carbonyl group, and a 3- to 14-membered non-aromatic heterocyclyloxy group, (2) a C₃₋₆ cycloalkyl group optionally substituted by 1 to 3 C₁₋₆ alkyl groups, (3) a C₂₋₆ alkenyl group, (4) a C₂₋₆ alkynyl group optionally substituted by 1 to 3 C₃₋₆ cycloalkyl groups, (5) a C₆₋₁₀ aryl group optionally substituted by 1 to 3 substituents selected from a halogen atom, an optionally halogenated C₁₋₆ alkyl group, and a C₁₋₆ alkoxy group, (6) a C₁₋₆ alkoxy group optionally substituted by 1 to 3 C₃₋₆ cycloalkoxy groups, (7) a C₃₋₆ cycloalkoxy group, or (8) a 4- to 6-membered heterocyclic group optionally substituted by 1 to 3 C₁₋₆ alkyl groups. [4] The compound or salt according to the above-mentioned [1], wherein R¹ is a C₁₋₆ alkyl group, a mono- or di-C₁₋₆ alkylamino group, or a C₃₋₆ cycloalkyl group; R² is a hydrogen atom; R³ is a C₁₋₆ alkyl group; and

R⁴ is

(1) a C₁₋₆ alkyl group, (2) a C₃₋₆ cycloalkyl group, (3) a C₂₋₆ alkenyl group, (4) a C₂₋₆ alkynyl group, (5) a C₆₋₁₀ aryl group, or (6) a C₁₋₆ alkoxy group. [5] The compound or salt according to claim 1, wherein R¹ is a C₁₋₆ alkyl group; R² is a hydrogen atom; R³ is a C₁₋₆ alkyl group; and

R⁴ is

(1) a C₁₋₆ alkyl group, (2) a C₂₋₆ alkynyl group, or (3) a C₁₋₆ alkoxy group. [6] N-[4-({[(1s,4S)-4-Ethoxycyclohexyl]oxy}methyl)-7-methyl-6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]methanesulfonamide or a salt thereof. [7] N-[7-Methyl-6-oxo-4-({[(1s,4S)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]ethanesulfonamide or a salt thereof. [8] N-[4-({[(1s,4S)-4-Ethylcyclohexyl]oxy}methyl)-7-methyl-6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]methanesulfonamide or a salt thereof. [9] A medicament comprising the compound or salt according to the above-mentioned [1]. [10] The medicament according to the above-mentioned [9], which is an orexin type 2 receptor agonist. [11] The medicament according to the above-mentioned [9], which is an agent for the prophylaxis or treatment of narcolepsy. [12] The compound or salt according to the above-mentioned [1] for use in the prophylaxis or treatment of narcolepsy. [13] A method for activating an orexin type 2 receptor in a mammal, which comprises administering an effective amount of the compound or salt according to the above-mentioned [1] to the mammal. [14] A method for preventing or treating narcolepsy in a mammal, which comprises administering an effective amount of the compound or salt according to the above-mentioned [1] to the mammal. [15] Use of the compound or salt according to the above-mentioned [1] for the manufacture of an agent for the prophylaxis or treatment of narcolepsy.

Effect of the Invention

The compound of the present invention has an orexin type 2 receptor agonist activity, and is useful as an agent for the prophylaxis or treatment of narcolepsy.

DETAILED DESCRIPTION OF THE INVENTION

The definition of each substituent used in the present specification is described in detail in the following. Unless otherwise specified, each substituent has the following definition.

In the present specification, examples of the “halogen atom” include fluorine, chlorine, bromine and iodine.

In the present specification, examples of the “C₁₋₆ alkyl group” include methyl, ethyl, propyl, isopropyl, butyl, isobutyl, sec-butyl, tert-butyl, pentyl, isopentyl, neopentyl, 1-ethylpropyl, hexyl, isohexyl, 1,1-dimethylbutyl, 2,2-dimethylbutyl, 3,3-dimethylbutyl and 2-ethylbutyl.

In the present specification, examples of the “optionally halogenated C₁₋₆ alkyl group” include a C₁₋₆ alkyl group optionally having 1 to 7, preferably 1 to 5, halogen atoms. Specific examples thereof include methyl, chloromethyl, difluoromethyl, trichloromethyl, trifluoromethyl, ethyl, 2-bromoethyl, 2,2,2-trifluoroethyl, tetrafluoroethyl, pentafluoroethyl, propyl, 2,2-difluoropropyl, 3,3,3-trifluoropropyl, isopropyl, butyl, 4,4,4-trifluorobutyl, isobutyl, sec-butyl, tert-butyl, pentyl, isopentyl, neopentyl, 5,5,5-trifluoropentyl, hexyl and 6,6,6-trifluorohexyl.

In the present specification, examples of the “C₂s alkenyl group” include ethenyl, 1-propenyl, 2-propenyl, 2-methyl-1-propenyl, 1-butenyl, 2-butenyl, 3-butenyl, 3-methyl-2-butenyl, 1-pentenyl, 2-pentenyl, 3-pentenyl, 4-pentenyl, 4-methyl-3-pentenyl, 1-hexenyl, 3-hexenyl and 5-hexenyl.

In the present specification, examples of the “C₂₋₆ alkynyl group” include ethynyl, 1-propynyl, 2-propynyl, 1-butynyl, 2-butynyl, 3-butynyl, 1-pentynyl, 2-pentynyl, 3-pentynyl, 4-pentynyl, 1-hexynyl, 2-hexynyl, 3-hexynyl, 4-hexynyl, 5-hexynyl and 4-methyl-2-pentynyl.

In the present specification, examples of the “C₃₋₁₀ cycloalkyl group” include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, bicyclo[2.2.1]heptyl, bicyclo[2.2.2]octyl, bicyclo[3.2.1]octyl and adamantyl. In the present specification, examples of the “optionally halogenated C₃₋₁₀ cycloalkyl group” include a C₃₋₁₀ cycloalkyl group optionally having 1 to 7, preferably 1 to 5, halogen atoms. Specific examples thereof include cyclopropyl, 2,2-difluorocyclopropyl, 2,3-difluorocyclopropyl, cyclobutyl, difluorocyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl and cyclooctyl.

In the present specification, examples of the “C₃₋₁₀ cycloalkenyl group” include cyclopropenyl, cyclobutenyl, cyclopentenyl, cyclohexenyl, cycloheptenyl and cyclooctenyl.

In the present specification, examples of the “C₆₋₁₄ aryl group” include phenyl, 1-naphthyl, 2-naphthyl, 1-anthryl, 2-anthryl and 9-anthryl.

In the present specification, examples of the “C₇₋₁₆ aralkyl group” include benzyl, phenethyl, naphthylmethyl and phenylpropyl.

In the present specification, examples of the “C₁₋₆ alkoxy group” include methoxy, ethoxy, propoxy, isopropoxy, butoxy, isobutoxy, sec-butoxy, tert-butoxy, pentyloxy and hexyloxy.

In the present specification, examples of the “optionally halogenated C₁₋₆ alkoxy group” include a C₁₋₆ alkoxy group optionally having 1 to 7, preferably 1 to 5, halogen atoms. Specific examples thereof include methoxy, difluoromethoxy, trifluoromethoxy, ethoxy, 2,2,2-trifluoroethoxy, propoxy, isopropoxy, butoxy, 4,4,4-trifluorobutoxy, isobutoxy, sec-butoxy, pentyloxy and hexyloxy.

In the present specification, examples of the “C₃₋₁₀ cycloalkyloxy group” include cyclopropyloxy, cyclobutyloxy, cyclopentyloxy, cyclohexyloxy, cycloheptyloxy and cyclooctyloxy.

In the present specification, examples of the “C₁₋₆ alkylthio group” include methylthio, ethylthio, propylthio, isopropylthio, butylthio, sec-butylthio, tert-butylthio, pentylthio and hexylthio.

In the present specification, examples of the “optionally halogenated C₁₋₆ alkylthio group” include a C₁₋₆ alkylthio group optionally having 1 to 7, preferably 1 to 5, halogen atoms. Specific examples thereof include methylthio, difluoromethylthio, trifluoromethylthio, ethylthio, propylthio, isopropylthio, butylthio, 4,4,4-trifluorobutylthio, pentylthio and hexylthio.

In the present specification, examples of the “C₁₋₆ alkyl-carbonyl group” include acetyl, propanoyl, butanoyl, 2-methylpropanoyl, pentanoyl, 3-methylbutanoyl, 2-methylbutanoyl, 2,2-dimethylpropanoyl, hexanoyl and heptanoyl.

In the present specification, examples of the “optionally halogenated C₁₋₆ alkyl-carbonyl group” include a C₁₋₆ alkyl-carbonyl group optionally having 1 to 7, preferably 1 to 5, halogen atoms. Specific examples thereof include acetyl, chloroacetyl, trifluoroacetyl, trichloroacetyl, propanoyl, butanoyl, pentanoyl and hexanoyl.

In the present specification, examples of the “C₁₋₆ alkoxy-carbonyl group” include methoxycarbonyl, ethoxycarbonyl, propoxycarbonyl, isopropoxycarbonyl, butoxycarbonyl, isobutoxycarbonyl, sec-butoxycarbonyl, tert-butoxycarbonyl, pentyloxycarbonyl and hexyloxycarbonyl.

In the present specification, examples of the “C₆₋₁₄ aryl-carbonyl group” include benzoyl, 1-naphthoyl and 2-naphthoyl.

In the present specification, examples of the “C₇₋₁₆ aralkyl-carbonyl group” include phenylacetyl and phenylpropionyl.

In the present specification, examples of the “5- to 14-membered aromatic heterocyclylcarbonyl group” include nicotinoyl, isonicotinoyl, thenoyl and furoyl.

In the present specification, examples of the “3- to 14-membered non-aromatic heterocyclylcarbonyl group” include morpholinylcarbonyl, piperidinylcarbonyl and pyrrolidinylcarbonyl.

In the present specification, examples of the “mono- or di-C₁₋₆ alkyl-carbamoyl group” include methylcarbamoyl, ethylcarbamoyl, dimethylcarbamoyl, diethylcarbamoyl and N-ethyl-N-methylcarbamoyl.

In the present specification, examples of the “mono- or di-C₇₋₁₆ aralkyl-carbamoyl group” include benzylcarbamoyl and phenethylcarbamoyl.

In the present specification, examples of the “C₁₋₆ alkylsulfonyl group” include methylsulfonyl, ethylsulfonyl, propylsulfonyl, isopropylsulfonyl, butylsulfonyl, sec-butylsulfonyl and tert-butylsulfonyl.

In the present specification, examples of the “optionally halogenated C₁₋₆ alkylsulfonyl group” include a C₁₋₆ alkylsulfonyl group optionally having 1 to 7, preferably 1 to 5, halogen atoms. Specific examples thereof include methylsulfonyl, difluoromethylsulfonyl, trifluoromethylsulfonyl, ethylsulfonyl, propylsulfonyl, isopropylsulfonyl, butylsulfonyl, 4,4,4-trifluorobutylsulfonyl, pentylsulfonyl and hexylsulfonyl.

In the present specification, examples of the “C₆₋₁₄ arylsulfonyl group” include phenylsulfonyl, 1-naphthylsulfonyl and 2-naphthylsulfonyl.

In the present specification, examples of the “substituent” include a halogen atom, a cyano group, a nitro group, an optionally substituted hydrocarbon group, an optionally substituted heterocyclic group, an acyl group, an optionally substituted amino group, an optionally substituted carbamoyl group, an optionally substituted thiocarbamoyl group, an optionally substituted sulfamoyl group, an optionally substituted hydroxy group, an optionally substituted sulfanyl (SH) group and an optionally substituted silyl group.

In the present specification, examples of the “hydrocarbon group” (including “hydrocarbon group” of “optionally substituted hydrocarbon group”) include a C₁₋₆ alkyl group, a C₂₋₆ alkenyl group, a C₂₋₆ alkynyl group, a C₃₋₁₀ cycloalkyl group, a C₃₋₁₀ cycloalkenyl group, a C₆₋₁₄ aryl group and a C₇₋₁₆ aralkyl group.

In the present specification, examples of the “optionally substituted hydrocarbon group” include a hydrocarbon group optionally having substituent(s) selected from the following Substituent Group A.

[Substituent Group A]

(1) a halogen atom, (2) a nitro group, (3) a cyano group, (4) an oxo group, (5) a hydroxy group, (6) an optionally halogenated C₁₋₆ alkoxy group, (7) a C₆₋₁₄ aryloxy group (e.g., phenoxy, naphthoxy), (8) a C₇₋₁₆ aralkyloxy group (e.g., benzyloxy), (9) a 5- to 14-membered aromatic heterocyclyloxy group (e.g., pyridyloxy), (10) a 3- to 14-membered non-aromatic heterocyclyloxy group (e.g., tetrahydropyranyloxy, morpholinyloxy, piperidinyloxy), (11) a C₁₋₆ alkyl-carbonyloxy group (e.g., acetoxy, propanoyloxy), (12) a C₆₋₁₄ aryl-carbonyloxy group (e.g., benzoyloxy, 1-naphthoyloxy, 2-naphthoyloxy), (13) a C₁₋₆ alkoxy-carbonyloxy group (e.g., methoxycarbonyloxy, ethoxycarbonyloxy, propoxycarbonyloxy, butoxycarbonyloxy), (14) a mono- or di-C₁₋₆ alkyl-carbamoyloxy group (e.g., methylcarbamoyloxy, ethylcarbamoyloxy, dimethylcarbamoyloxy, diethylcarbamoyloxy), (15) a C₆₋₁₄ aryl-carbamoyloxy group (e.g., phenylcarbamoyloxy, naphthylcarbamoyloxy), (16) a 5- to 14-membered aromatic heterocyclylcarbonyloxy group (e.g., nicotinoyloxy), (17) a 3- to 14-membered non-aromatic heterocyclylcarbonyloxy group (e.g., morpholinylcarbonyloxy, piperidinylcarbonyloxy), (18) an optionally halogenated C₁₋₆ alkylsulfonyloxy group (e.g., methylsulfonyloxy, trifluoromethylsulfonyloxy), (19) a C₆₋₁₄ arylsulfonyloxy group optionally substituted by a C₁₋₆ alkyl group (e.g., phenylsulfonyloxy, toluenesulfonyloxy), (20) an optionally halogenated C₁₋₆ alkylthio group, (21) a 5- to 14-membered aromatic heterocyclic group, (22) a 3- to 14-membered non-aromatic heterocyclic group, (23) a formyl group, (24) a carboxy group, (25) an optionally halogenated C₁₋₆ alkyl-carbonyl group, (26) a C₆₋₁₄ aryl-carbonyl group, (27) a 5- to 14-membered aromatic heterocyclylcarbonyl group, (28) a 3- to 14-membered non-aromatic heterocyclylcarbonyl group, (29) a C₁₋₆ alkoxy-carbonyl group, (30) a C₆₋₁₄ aryloxy-carbonyl group (e.g., phenyloxycarbonyl, 1-naphthyloxycarbonyl, 2-naphthyloxycarbonyl), (31) a C₇₋₁₆ aralkyloxy-carbonyl group (e.g., benzyloxycarbonyl, phenethyloxycarbonyl), (32) a carbamoyl group, (33) a thiocarbamoyl group, (34) a mono- or di-C₁₋₆ alkyl-carbamoyl group, (35) a C₆₋₁₄ aryl-carbamoyl group (e.g., phenylcarbamoyl), (36) a 5- to 14-membered aromatic heterocyclylcarbamoyl group (e.g., pyridylcarbamoyl, thienylcarbamoyl), (37) a 3- to 14-membered non-aromatic heterocyclylcarbamoyl group (e.g., morpholinylcarbamoyl, piperidinylcarbamoyl), (38) an optionally halogenated C₁₋₆ alkylsulfonyl group, (39) a C₆₋₁₄ arylsulfonyl group, (40) a 5- to 14-membered aromatic heterocyclylsulfonyl group (e.g., pyridylsulfonyl, thienylsulfonyl), (41) an optionally halogenated C₁₋₆ alkylsulfinyl group, (42) a C₆₋₁₄ arylsulfinyl group (e.g., phenylsulfinyl, 1-naphthylsulfinyl, 2-naphthylsulfinyl), (43) a 5- to 14-membered aromatic heterocyclylsulfinyl group (e.g., pyridylsulfinyl, thienylsulfinyl), (44) an amino group, (45) a mono- or di-C₁₋₆ alkylamino group (e.g., methylamino, ethylamino, propylamino, isopropylamino, butylamino, dimethylamino, diethylamino, dipropylamino, dibutylamino, N-ethyl-N-methylamino), (46) a mono- or di-C₆₋₁₄ arylamino group (e.g., phenylamino), (47) a 5- to 14-membered aromatic heterocyclylamino group (e.g., pyridylamino), (48) a C₇₋₁₆ aralkylamino group (e.g., benzylamino), (49) a formylamino group, (50) a C₁₋₆ alkyl-carbonylamino group (e.g., acetylamino, propanoylamino, butanoylamino), (51) a (C₁₋₆ alkyl) (C₁₋₆ alkyl-carbonyl) amino group (e.g., N-acetyl-N-methylamino), (52) a C₆₋₁₄ aryl-carbonylamino group (e.g., phenylcarbonylamino, naphthylcarbonylamino), (53) a C₁₋₆ alkoxy-carbonylamino group (e.g., methoxycarbonylamino, ethoxycarbonylamino, propoxycarbonylamino, butoxycarbonylamino, tert-butoxycarbonylamino), (54) a C₇₋₁₆ aralkyloxy-carbonylamino group (e.g., benzyloxycarbonylamino), (55) a C₁₋₆ alkylsulfonylamino group (e.g., methylsulfonylamino, ethylsulfonylamino), (56) a C₆₋₁₄ arylsulfonylamino group optionally substituted by a C₁₋₆ alkyl group (e.g., phenylsulfonylamino, toluenesulfonylamino), (57) an optionally halogenated C₁₋₆ alkyl group, (58) a C₂₋₆ alkenyl group, (59) a C₂₋₆ alkynyl group, (60) a C₃₋₁₀ cycloalkyl group, (61) a C₃₋₁₀ cycloalkenyl group, and (62) a C₆₋₁₄ aryl group.

The number of the above-mentioned substituents in the “optionally substituted hydrocarbon group” is, for example, 1 to 5, preferably 1 to 3. When the number of the substituents is two or more, the respective substituents may be the same or different.

In the present specification, examples of the “heterocyclic group” (including “heterocyclic group” of “optionally substituted heterocyclic group”) include (i) an aromatic heterocyclic group, (ii) a non-aromatic heterocyclic group and (iii) a 7- to 10-membered bridged heterocyclic group, each containing, as a ring-constituting atom besides carbon atom, 1 to 4 heteroatoms selected from a nitrogen atom, a sulfur atom and an oxygen atom.

In the present specification, examples of the “aromatic heterocyclic group” (including “5- to 14-membered aromatic heterocyclic group”) include a 5- to 14-membered (preferably 5- to 10-membered) aromatic heterocyclic group containing, as a ring-constituting atom besides carbon atom, 1 to 4 heteroatoms selected from a nitrogen atom, a sulfur atom and an oxygen atom.

Preferable examples of the “aromatic heterocyclic group” include 5- or 6-membered monocyclic aromatic heterocyclic groups such as thienyl, furyl, pyrrolyl, imidazolyl, pyrazolyl, thiazolyl, isothiazolyl, oxazolyl, isoxazolyl, pyridyl, pyrazinyl, pyrimidinyl, pyridazinyl, 1,2,4-oxadiazolyl, 1,3,4-oxadiazolyl, 1,2,4-thiadiazolyl, 1,3,4-thiadiazolyl, triazolyl, tetrazolyl, triazinyl and the like; and 8- to 14-membered fused polycyclic (preferably bi- or tri-cyclic) aromatic heterocyclic groups such as benzothiophenyl, benzofuranyl, benzimidazolyl, benzoxazolyl, benzisoxazolyl, benzothiazolyl, benzisothiazolyl, benzotriazolyl, imidazopyridinyl, thienopyridinyl, furopyridinyl, pyrrolopyridinyl, pyrazolopyridinyl, oxazolopyridinyl, thiazolopyridinyl, imidazopyrazinyl, imidazopyrimidinyl, thienopyrimidinyl, furopyrimidinyl, pyrrolopyrimidinyl, pyrazolopyrimidinyl, oxazolopyrimidinyl, thiazolopyrimidinyl, pyrazolotriazinyl, naphtho[2,3-b]thienyl, phenoxathiinyl, indolyl, isoindolyl, 1H-indazolyl, purinyl, isoquinolyl, quinolyl, phthalazinyl, naphthyridinyl, quinoxalinyl, quinazolinyl, cinnolinyl, carbazolyl, β-carbolinyl, phenanthridinyl, acridinyl, phenazinyl, phenothiazinyl, phenoxazinyl and the like.

In the present specification, examples of the “non-aromatic heterocyclic group” (including “3- to 14-membered non-aromatic heterocyclic group”) include a 3- to 14-membered (preferably 4- to 10-membered) non-aromatic heterocyclic group containing, as a ring-constituting atom besides carbon atom, 1 to 4 heteroatoms selected from a nitrogen atom, a sulfur atom and an oxygen atom.

Preferable examples of the “non-aromatic heterocyclic group” include 3- to 8-membered monocyclic non-aromatic heterocyclic groups such as aziridinyl, oxiranyl, thiiranyl, azetidinyl, oxetanyl, thietanyl, tetrahydrothienyl, tetrahydrofuranyl, pyrrolinyl, pyrrolidinyl, imidazolinyl, imidazolidinyl, oxazolinyl, oxazolidinyl, pyrazolinyl, pyrazolidinyl, thiazolinyl, thiazolidinyl, tetrahydroisothiazolyl, tetrahydrooxazolyl, tetrahydroisooxazolyl, piperidinyl, piperazinyl, tetrahydropyridinyl, dihydropyridinyl, dihydrothiopyranyl, tetrahydropyrimidinyl, tetrahydropyridazinyl, dihydropyranyl, tetrahydropyranyl, tetrahydrothiopyranyl, morpholinyl, thiomorpholinyl, azepanyl, diazepanyl, azepinyl, oxepanyl, azocanyl, diazocanyl and the like; and 9- to 14-membered fused polycyclic (preferably bi- or tri-cyclic) non-aromatic heterocyclic groups such as dihydrobenzofuranyl, dihydrobenzimidazolyl, dihydrobenzoxazolyl, dihydrobenzothiazolyl, dihydrobenzisothiazolyl, dihydronaphtho[2,3-b]thienyl, tetrahydroisoquinolyl, tetrahydroquinolyl, 4H-quinolizinyl, indolinyl, isoindolinyl, tetrahydrothieno[2,3-c]pyridinyl, tetrahydrobenzazepinyl, tetrahydroquinoxalinyl, tetrahydrophenanthridinyl, hexahydrophenothiazinyl, hexahydrophenoxazinyl, tetrahydrophthalazinyl, tetrahydronaphthyridinyl, tetrahydroquinazolinyl, tetrahydrocinnolinyl, tetrahydrocarbazolyl, tetrahydro-β-carbolinyl, tetrahydroacrydinyl, tetrahydrophenazinyl, tetrahydrothioxanthenyl, octahydroisoquinolyl and the like.

In the present specification, preferable examples of the “7- to 10-membered bridged heterocyclic group” include quinuclidinyl and 7-azabicyclo[2.2.1]heptanyl.

In the present specification, examples of the “nitrogen-containing heterocyclic group” include a “heterocyclic group” containing at least one nitrogen atom as a ring-constituting atom.

In the present specification, examples of the “optionally substituted heterocyclic group” include a heterocyclic group optionally having substituent(s) selected from the above-mentioned Substituent Group A.

The number of the substituents in the “optionally substituted heterocyclic group” is, for example, 1 to 3. When the number of the substituents is two or more, the respective substituents may be the same or different.

In the present specification, examples of the “acyl group” include a formyl group, a carboxy group, a carbamoyl group, a thiocarbamoyl group, a sulfino group, a sulfo group, a sulfamoyl group and a phosphono group, each optionally having “1 or 2 substituents selected from a C₁₋₆ alkyl group, a C₂₋₆ alkenyl group, a C₃₋₁₀ cycloalkyl group, a C₃₋₁₀ cycloalkenyl group, a C₆₋₁₄ aryl group, a C₇₋₁₆ aralkyl group, a 5- to 14-membered aromatic heterocyclic group and a 3- to 14-membered non-aromatic heterocyclic group, each of which optionally has 1 to 3 substituents selected from a halogen atom, an optionally halogenated C₁₋₆ alkoxy group, a hydroxy group, a nitro group, a cyano group, an amino group and a carbamoyl group”.

Examples of the “acyl group” also include a hydrocarbon-sulfonyl group, a heterocyclylsulfonyl group, a hydrocarbon-sulfinyl group and a heterocyclylsulfinyl group.

Here, the hydrocarbon-sulfonyl group means a hydrocarbon group-bonded sulfonyl group, the heterocyclylsulfonyl group means a heterocyclic group-bonded sulfonyl group, the hydrocarbon-sulfinyl group means a hydrocarbon group-bonded sulfinyl group and the heterocyclylsulfinyl group means a heterocyclic group-bonded sulfinyl group.

Preferable examples of the “acyl group” include a formyl group, a carboxy group, a C₁₋₆ alkyl-carbonyl group, a C₂₋₆ alkenyl-carbonyl group (e.g., crotonoyl), a C₃₋₁₀ cycloalkyl-carbonyl group (e.g., cyclobutanecarbonyl, cyclopentanecarbonyl, cyclohexanecarbonyl, cycloheptanecarbonyl), a C₃₋₁₀ cycloalkenyl-carbonyl group (e.g., 2-cyclohexenecarbonyl), a C₆₋₁₄ aryl-carbonyl group, a C₇₋₁₆ aralkyl-carbonyl group, a 5- to 14-membered aromatic heterocyclylcarbonyl group, a 3- to 14-membered non-aromatic heterocyclylcarbonyl group, a C₁₋₆ alkoxy-carbonyl group, a C₆₋₁₄ aryloxy-carbonyl group (e.g., phenyloxycarbonyl, naphthyloxycarbonyl), a C₇₋₁₆ aralkyloxy-carbonyl group (e.g., benzyloxycarbonyl, phenethyloxycarbonyl), a carbamoyl group, a mono- or di-C₁₋₆ alkyl-carbamoyl group, a mono- or di-C₂₋₆ alkenyl-carbamoyl group (e.g., diallylcarbamoyl), a mono- or di-C₃₋₁₀ cycloalkyl-carbamoyl group (e.g., cyclopropylcarbamoyl), a mono- or di-C₆₋₁₄ aryl-carbamoyl group (e.g., phenylcarbamoyl), a mono- or di-C₇₋₁₆ aralkyl-carbamoyl group, a 5- to 14-membered aromatic heterocyclylcarbamoyl group (e.g., pyridylcarbamoyl), a thiocarbamoyl group, a mono- or di-C₁₋₆ alkyl-thiocarbamoyl group (e.g., methylthiocarbamoyl, N-ethyl-N-methylthiocarbamoyl), a mono- or di-C₂₋₆ alkenyl-thiocarbamoyl group (e.g., diallylthiocarbamoyl), a mono- or di-C₃₋₁₀ cycloalkyl-thiocarbamoyl group (e.g., cyclopropylthiocarbamoyl, cyclohexylthiocarbamoyl), a mono- or di-C₆₋₁₄ aryl-thiocarbamoyl group (e.g., phenylthiocarbamoyl), a mono- or di-C₇₋₁₆ aralkyl-thiocarbamoyl group (e.g., benzylthiocarbamoyl, phenethylthiocarbamoyl), a 5- to 14-membered aromatic heterocyclylthiocarbamoyl group (e.g., pyridylthiocarbamoyl), a sulfino group, a C₁₋₆ alkylsulfinyl group (e.g., methylsulfinyl, ethylsulfinyl), a sulfo group, a C₁₋₆ alkylsulfonyl group, a C₆₋₁₄ arylsulfonyl group, a phosphono group and a mono- or di-C₁₋₆ alkylphosphono group (e.g., dimethylphosphono, diethylphosphono, diisopropylphosphono, dibutylphosphono).

In the present specification, examples of the “optionally substituted amino group” include an amino group optionally having “1 or 2 substituents selected from a C₁₋₆ alkyl group, a C₂₋₆ alkenyl group, a C₃₋₁₀ cycloalkyl group, a C₆₋₁₄ aryl group, a C₇₋₁₆ aralkyl group, a C₁₋₆ alkyl-carbonyl group, a C₆₋₁₄ aryl-carbonyl group, a C₇₋₁₆ aralkyl-carbonyl group, a 5- to 14-membered aromatic heterocyclylcarbonyl group, a 3- to 14-membered non-aromatic heterocyclylcarbonyl group, a C₁₋₆ alkoxy-carbonyl group, a 5- to 14-membered aromatic heterocyclic group, a carbamoyl group, a mono- or di-C₁₋₆ alkyl-carbamoyl group, a mono- or di-C₇₋₁₆ aralkyl-carbamoyl group, a C₁₋₆ alkylsulfonyl group and a C₆₋₁₄ arylsulfonyl group, each of which optionally has 1 to 3 substituents selected from Substituent Group A”.

Preferable examples of the optionally substituted amino group include an amino group, a mono- or di-(optionally halogenated C₁₋₆ alkyl) amino group (e.g., methylamino, trifluoromethylamino, dimethylamino, ethylamino, diethylamino, propylamino, dibutylamino), a mono- or di-C₂₋₆ alkenylamino group (e.g., diallylamino), a mono- or di-C₃₋₁₀ cycloalkylamino group (e.g., cyclopropylamino, cyclohexylamino), a mono- or di-C₆₋₁₄ arylamino group (e.g., phenylamino), a mono- or di-C₇₋₁₆ aralkylamino group (e.g., benzylamino, dibenzylamino), a mono- or di-(optionally halogenated C₁₋₆ alkyl)-carbonylamino group (e.g., acetylamino, propionylamino), a mono- or di-C₆₋₁₄ aryl-carbonylamino group (e.g., benzoylamino), a mono- or di-C₇₋₁₆ aralkyl-carbonylamino group (e.g., benzylcarbonylamino), a mono- or di-5- to 14-membered aromatic heterocyclylcarbonylamino group (e.g., nicotinoylamino, isonicotinoylamino), a mono- or di-3- to 14-membered non-aromatic heterocyclylcarbonylamino group (e.g., piperidinylcarbonylamino), a mono- or di-C₁₋₆ alkoxy-carbonylamino group (e.g., tert-butoxycarbonylamino), a 5- to 14-membered aromatic heterocyclylamino group (e.g., pyridylamino), a carbamoylamino group, a (mono- or di-C₁₋₆ alkyl-carbamoyl) amino group (e.g., methylcarbamoylamino), a (mono- or di-C₇₋₁₆ aralkyl-carbamoyl) amino group (e.g., benzylcarbamoylamino), a C₁₋₆ alkylsulfonylamino group (e.g., methylsulfonylamino, ethylsulfonylamino), a C₆₋₁₄ arylsulfonylamino group (e.g., phenylsulfonylamino), a (C₁₋₆ alkyl) (C₁₋₆ alkyl-carbonyl) amino group (e.g., N-acetyl-N-methylamino) and a (C₁₋₆ alkyl) (C₆₋₁₄ aryl-carbonyl) amino group (e.g., N-benzoyl-N-methylamino).

In the present specification, examples of the “optionally substituted carbamoyl group” include a carbamoyl group optionally having “1 or 2 substituents selected from a C₁₋₆ alkyl group, a C₂₋₆ alkenyl group, a C₃₋₁₀ cycloalkyl group, a C₆₋₁₄ aryl group, a C₇₋₁₆ aralkyl group, a C₁₋₆ alkyl-carbonyl group, a C₆₋₁₄ aryl-carbonyl group, a C₇₋₁₆ aralkyl-carbonyl group, a 5- to 14-membered aromatic heterocyclylcarbonyl group, a 3- to 14-membered non-aromatic heterocyclylcarbonyl group, a C₁₋₆ alkoxy-carbonyl group, a 5- to 14-membered aromatic heterocyclic group, a carbamoyl group, a mono- or di-C₁₋₆ alkyl-carbamoyl group and a mono- or di-C₇₋₁₆ aralkyl-carbamoyl group, each of which optionally has 1 to 3 substituents selected from Substituent Group A”.

Preferable examples of the optionally substituted carbamoyl group include a carbamoyl group, a mono- or di-C₁₋₆ alkyl-carbamoyl group, a mono- or di-C₂₋₆ alkenyl-carbamoyl group (e.g., diallylcarbamoyl), a mono- or di-C₃₋₁₀ cycloalkyl-carbamoyl group (e.g., cyclopropylcarbamoyl, cyclohexylcarbamoyl), a mono- or di-C₆₋₁₄ aryl-carbamoyl group (e.g., phenylcarbamoyl), a mono- or di-C₇₋₁₆ aralkyl-carbamoyl group, a mono- or di-C₁₋₆ alkyl-carbonyl-carbamoyl group (e.g., acetylcarbamoyl, propionylcarbamoyl), a mono- or di-C₆₋₁₄ aryl-carbonyl-carbamoyl group (e.g., benzoylcarbamoyl) and a 5- to 14-membered aromatic heterocyclylcarbamoyl group (e.g., pyridylcarbamoyl).

In the present specification, examples of the “optionally substituted thiocarbamoyl group” include a thiocarbamoyl group optionally having “1 or 2 substituents selected from a C₁₋₆ alkyl group, a C₂₋₆ alkenyl group, a C₃₋₁₀ cycloalkyl group, a C₆₋₁₄ aryl group, a C₇₋₁₆ aralkyl group, a C₁₋₆ alkyl-carbonyl group, a C₆₋₁₄ aryl-carbonyl group, a C₇₋₁₆ aralkyl-carbonyl group, a 5- to 14-membered aromatic heterocyclylcarbonyl group, a 3- to 14-membered non-aromatic heterocyclylcarbonyl group, a C₁₋₆ alkoxy-carbonyl group, a 5- to 14-membered aromatic heterocyclic group, a carbamoyl group, a mono- or di-C₁₋₆ alkyl-carbamoyl group and a mono- or di-C₇₋₁₆ aralkyl-carbamoyl group, each of which optionally has 1 to 3 substituents selected from Substituent Group A”.

Preferable examples of the optionally substituted thiocarbamoyl group include a thiocarbamoyl group, a mono- or di-C₁₋₆ alkyl-thiocarbamoyl group (e.g., methylthiocarbamoyl, ethylthiocarbamoyl, dimethylthiocarbamoyl, diethylthiocarbamoyl, N-ethyl-N-methylthiocarbamoyl), a mono- or di-C₂₋₆ alkenyl-thiocarbamoyl group (e.g., diallylthiocarbamoyl), a mono- or di-C₃₋₁₀ cycloalkyl-thiocarbamoyl group (e.g., cyclopropylthiocarbamoyl, cyclohexylthiocarbamoyl), a mono- or di-C₆₋₁₄ aryl-thiocarbamoyl group (e.g., phenylthiocarbamoyl), a mono- or di-C₇₋₁₆ aralkyl-thiocarbamoyl group (e.g., benzylthiocarbamoyl, phenethylthiocarbamoyl), a mono- or di-C₁₋₆ alkyl-carbonyl-thiocarbamoyl group (e.g., acetylthiocarbamoyl, propionylthiocarbamoyl), a mono- or di-C₆₋₁₄ aryl-carbonyl-thiocarbamoyl group (e.g., benzoylthiocarbamoyl) and a 5- to 14-membered aromatic heterocyclylthiocarbamoyl group (e.g., pyridylthiocarbamoyl).

In the present specification, examples of the “optionally substituted sulfamoyl group” include a sulfamoyl group optionally having “1 or 2 substituents selected from a C₁₋₆ alkyl group, a C₂₋₆ alkenyl group, a C₃₋₁₀ cycloalkyl group, a C₆₋₁₄ aryl group, a C₇₋₁₆ aralkyl group, a C₁₋₆ alkyl-carbonyl group, a C₆₋₁₄ aryl-carbonyl group, a C₇₋₁₆ aralkyl-carbonyl group, a 5- to 14-membered aromatic heterocyclylcarbonyl group, a 3- to 14-membered non-aromatic heterocyclylcarbonyl group, a C₁₋₆ alkoxy-carbonyl group, a 5- to 14-membered aromatic heterocyclic group, a carbamoyl group, a mono- or di-C₁₋₆ alkyl-carbamoyl group and a mono- or di-C₇₋₁₆ aralkyl-carbamoyl group, each of which optionally has 1 to 3 substituents selected from Substituent Group A”.

Preferable examples of the optionally substituted sulfamoyl group include a sulfamoyl group, a mono- or di-C₁₋₆ alkyl-sulfamoyl group (e.g., methylsulfamoyl, ethylsulfamoyl, dimethylsulfamoyl, diethylsulfamoyl, N-ethyl-N-methylsulfamoyl), a mono- or di-C₂₋₆ alkenyl-sulfamoyl group (e.g., diallylsulfamoyl), a mono- or di-C₃₋₁₀ cycloalkyl-sulfamoyl group (e.g., cyclopropylsulfamoyl, cyclohexylsulfamoyl), a mono- or di-C₆₋₁₄ aryl-sulfamoyl group (e.g., phenylsulfamoyl), a mono- or di-C₇₋₁₆ aralkyl-sulfamoyl group (e.g., benzylsulfamoyl, phenethylsulfamoyl), a mono- or di-C₁₋₆ alkyl-carbonyl-sulfamoyl group (e.g., acetylsulfamoyl, propionylsulfamoyl), a mono- or di-C₆₋₁₄ aryl-carbonyl-sulfamoyl group (e.g., benzoylsulfamoyl) and a 5- to 14-membered aromatic heterocyclylsulfamoyl group (e.g., pyridylsulfamoyl).

In the present specification, examples of the “optionally substituted hydroxy group” include a hydroxy group optionally having “a substituent selected from a C₁₋₆ alkyl group, a C₂₋₆ alkenyl group, a C₃₋₁₀ cycloalkyl group, a C₆₋₁₄ aryl group, a C₇₋₁₆ aralkyl group, a C₁₋₆ alkyl-carbonyl group, a C₆₋₁₄ aryl-carbonyl group, a C₇₋₁₆ aralkyl-carbonyl group, a 5- to 14-membered aromatic heterocyclylcarbonyl group, a 3- to 14-membered non-aromatic heterocyclylcarbonyl group, a C₁₋₆ alkoxy-carbonyl group, a 5- to 14-membered aromatic heterocyclic group, a carbamoyl group, a mono- or di-C₁₋₆ alkyl-carbamoyl group, a mono- or di-C₇₋₁₆ aralkyl-carbamoyl group, a C₁₋₆ alkylsulfonyl group and a C₆₋₁₄ arylsulfonyl group, each of which optionally has 1 to 3 substituents selected from Substituent Group A”.

Preferable examples of the optionally substituted hydroxy group include a hydroxy group, a C₁₋₆ alkoxy group, a C₂₋₆ alkenyloxy group (e.g., allyloxy, 2-butenyloxy, 2-pentenyloxy, 3-hexenyloxy), a C₃₋₁₀ cycloalkyloxy group (e.g., cyclohexyloxy), a C₆₋₁₄ aryloxy group (e.g., phenoxy, naphthyloxy), a C₇₋₁₆ aralkyloxy group (e.g., benzyloxy, phenethyloxy), a C₁₋₆ alkyl-carbonyloxy group (e.g., acetyloxy, propionyloxy, butyryloxy, isobutyryloxy, pivaloyloxy), a C₆₋₁₄ aryl-carbonyloxy group (e.g., benzoyloxy), a C₇₋₁₆ aralkyl-carbonyloxy group (e.g., benzylcarbonyloxy), a 5- to 14-membered aromatic heterocyclylcarbonyloxy group (e.g., nicotinoyloxy), a 3- to 14-membered non-aromatic heterocyclylcarbonyloxy group (e.g., piperidinylcarbonyloxy), a C₁₋₆ alkoxy-carbonyloxy group (e.g., tert-butoxycarbonyloxy), a 5- to 14-membered aromatic heterocyclyloxy group (e.g., pyridyloxy), a carbamoyloxy group, a C₁₋₆ alkyl-carbamoyloxy group (e.g., methylcarbamoyloxy), a C₇₋₁₆ aralkyl-carbamoyloxy group (e.g., benzylcarbamoyloxy), a C₁₋₆ alkylsulfonyloxy group (e.g., methylsulfonyloxy, ethylsulfonyloxy) and a C₆₋₁₄ arylsulfonyloxy group (e.g., phenylsulfonyloxy).

In the present specification, examples of the “optionally substituted sulfanyl group” include a sulfanyl group optionally having “a substituent selected from a C₁₋₆ alkyl group, a C₂₋₆ alkenyl group, a C₃₋₁₀ cycloalkyl group, a C₆₋₁₄ aryl group, a C₇₋₁₆ aralkyl group, a C₁₋₆ alkyl-carbonyl group, a C₆₋₁₄ aryl-carbonyl group and a 5- to 14-membered aromatic heterocyclic group, each of which optionally has 1 to 3 substituents selected from Substituent Group A” and a halogenated sulfanyl group.

Preferable examples of the optionally substituted sulfanyl group include a sulfanyl (—SH) group, a C₁₋₆ alkylthio group, a C₂₋₆ alkenylthio group (e.g., allylthio, 2-butenylthio, 2-pentenylthio, 3-hexenylthio), a C₃₋₁₀ cycloalkylthio group (e.g., cyclohexylthio), a C₆₋₁₄ arylthio group (e.g., phenylthio, naphthylthio), a C₇₋₁₆ aralkylthio group (e.g., benzylthio, phenethylthio), a C₁₋₆ alkyl-carbonylthio group (e.g., acetylthio, propionylthio, butyrylthio, isobutyrylthio, pivaloylthio), a C₆₋₁₄ aryl-carbonylthio group (e.g., benzoylthio), a 5- to 14-membered aromatic heterocyclylthio group (e.g., pyridylthio) and a halogenated thio group (e.g., pentafluorothio).

In the present specification, examples of the “optionally substituted silyl group” include a silyl group optionally having “1 to 3 substituents selected from a C₁₋₆ alkyl group, a C₂₋₆ alkenyl group, a C₃₋₁₀ cycloalkyl group, a C₆₋₁₄ aryl group and a C₇₋₁₆ aralkyl group, each of which optionally has 1 to 3 substituents selected from Substituent Group A”.

Preferable examples of the optionally substituted silyl group include a tri-C₁₋₆ alkylsilyl group (e.g., trimethylsilyl, tert-butyl(dimethyl)silyl).

In the present specification, examples of the “hydrocarbon ring” include a C₆₋₁₄ aromatic hydrocarbon ring, C₃₋₁₀ cycloalkane and C₃₋₁₀ cycloalkene.

In the present specification, examples of the “C₆₋₁₄ aromatic hydrocarbon ring” include benzene and naphthalene.

In the present specification, examples of the “C₃₋₁₀ cycloalkane” include cyclopropane, cyclobutane, cyclopentane, cyclohexane, cycloheptane and cyclooctane.

In the present specification, examples of the “C₃₋₁₀ cycloalkene” include cyclopropene, cyclobutene, cyclopentene, cyclohexene, cycloheptene and cyclooctene.

In the present specification, examples of the “heterocycle” include an aromatic heterocycle and a non-aromatic heterocycle, each containing, as a ring-constituting atom besides carbon atom, 1 to 4 hetero atoms selected from a nitrogen atom, a sulfur atom and an oxygen atom.

In the present specification, examples of the “aromatic heterocycle” include a 5- to 14-membered (preferably 5- to 10-membered) aromatic heterocycle containing, as a ring-constituting atom besides carbon atom, 1 to 4 hetero atoms selected from a nitrogen atom, a sulfur atom and an oxygen atom. Preferable examples of the “aromatic heterocycle” include 5- or 6-membered monocyclic aromatic heterocycles such as thiophene, furan, pyrrole, imidazole, pyrazole, thiazole, isothiazole, oxazole, isoxazole, pyridine, pyrazine, pyrimidine, pyridazine, 1,2,4-oxadiazole, 1,3,4-oxadiazole, 1,2,4-thiadiazole, 1,3,4-thiadiazole, triazole, tetrazole, triazine and the like; and 8- to 14-membered fused polycyclic (preferably bi- or tri-cyclic) aromatic heterocycles such as benzothiophene, benzofuran, benzimidazole, benzoxazole, benzisoxazole, benzothiazole, benzisothiazole, benzotriazole, imidazopyridine, thienopyridine, furopyridine, pyrrolopyridine, pyrazolopyridine, oxazolopyridine, thiazolopyridine, imidazopyrazine, imidazopyrimidine, thienopyrimidine, furopyrimidine, pyrrolopyrimidine, pyrazolopyrimidine, oxazolopyrimidine, thiazolopyrimidine, pyrazolopyrimidine, pyrazolotriazine, naphtho[2,3-b]thiophene, phenoxathiin, indole, isoindole, 1H-indazole, purine, isoquinoline, quinoline, phthalazine, naphthyridine, quinoxaline, quinazoline, cinnoline, carbazole, β-carboline, phenanthridine, acridine, phenazine, phenothiazine, phenoxazine and the like.

In the present specification, examples of the “non-aromatic heterocycle” include a 3- to 14-membered (preferably 4- to 10-membered) non-aromatic heterocycle containing, as a ring-constituting atom besides carbon atom, 1 to 4 hetero atoms selected from a nitrogen atom, a sulfur atom and an oxygen atom. Preferable examples of the “non-aromatic heterocycle” include 3- to 8-membered monocyclic non-aromatic heterocycles such as aziridine, oxirane, thiirane, azetidine, oxetane, thietane, tetrahydrothiophene, tetrahydrofuran, pyrroline, pyrrolidine, imidazoline, imidazolidine, oxazoline, oxazolidine, pyrazoline, pyrazolidine, thiazoline, thiazolidine, tetrahydroisothiazole, tetrahydrooxazole, tetrahydroisoxazole, piperidine, piperazine, tetrahydropyridine, dihydropyridine, dihydrothiopyran, tetrahydropyrimidine, tetrahydropyridazine, dihydropyran, tetrahydropyran, tetrahydrothiopyran, morpholine, thiomorpholine, azepane, diazepane, azepine, azocane, diazocane, oxepane and the like; and 9- to 14-membered fused polycyclic (preferably bi- or tri-cyclic) non-aromatic heterocycles such as dihydrobenzofuran, dihydrobenzimidazole, dihydrobenzoxazole, dihydrobenzothiazole, dihydrobenzisothiazole, dihydronaphtho[2,3-b]thiophene, tetrahydroisoquinoline, tetrahydroquinoline, 4H-quinolizine, indoline, isoindoline, tetrahydrothieno[2,3-c]pyridine, tetrahydrobenzazepine, tetrahydroquinoxaline, tetrahydrophenanthridine, hexahydrophenothiazine, hexahydrophenoxazine, tetrahydrophthalazine, tetrahydronaphthyridine, tetrahydroquinazoline, tetrahydrocinnoline, tetrahydrocarbazole, tetrahydro-β-carboline, tetrahydroacridine, tetrahydrophenazine, tetrahydrothioxanthene, octahydroisoquinoline and the like.

In the present specification, examples of the “nitrogen-containing heterocycle” include a heterocycle containing at least one nitrogen atom as a ring-constituting atom, from among the “heterocycle”.

In the present specification, examples of the “4- to 6-membered heterocyclic group” include an aromatic or non-aromatic 4- to 6-membered heterocyclic group. Specific examples thereof include oxetanyl, furyl, pyrazolyl, pyridyl and pyrimidinyl.

The definition of each symbol in the formula (I) is explained in detail.

R¹ is an optionally substituted C₁₋₆ alkyl group, an optionally substituted mono- or di-C₁₋₆ alkylamino group, or an optionally substituted C₃₋₆ cycloalkyl group.

R¹ is preferably a C₁₋₆ alkyl group (e.g., methyl, ethyl), a mono- or di-C₁₋₆ alkylamino group (e.g., methylamino, dimethylamino), or a C₃₋₆ cycloalkyl group (e.g., cyclopropyl).

R¹ is particularly preferably a C₁₋₆ alkyl group (e.g., methyl, ethyl).

R² is a hydrogen atom, a halogen atom, a cyano group, an optionally substituted C₁₋₆ alkyl group, an optionally substituted C₃₋₆ cycloalkyl group, or an optionally substituted C₁₋₆ alkoxy group.

R² is preferably a hydrogen atom, a halogen atom (e.g., a fluorine atom, a bromine atom), a cyano group, a C₁₋₆ alkyl group (e.g., methyl), a C₃₋₆ cycloalkyl group (e.g., cyclopropyl), or a C₁₋₆ alkoxy group (e.g., methoxy).

R² is particularly preferably a hydrogen atom.

R³ is a hydrogen atom, a halogen atom, a cyano group, an optionally substituted C₁₋₆ alkyl group, an optionally substituted C₃₋₆ cycloalkyl group, or an optionally substituted C₁₋₆ alkoxy group.

R³ is preferably a hydrogen atom, a halogen atom (e.g., a fluorine atom, a bromine atom), a cyano group, a C₁₋₆ alkyl group optionally substituted by 1 to 3 hydroxy groups (e.g., methyl, ethyl, isopropyl), a C₃₋₆ cycloalkyl group (e.g., cyclopropyl), or a C₁₋₆ alkoxy group (e.g., methoxy).

R³ is particularly preferably a C₁₋₆ alkyl group (e.g., methyl).

R⁴ is an optionally substituted C₁₋₆ alkyl group, an optionally substituted C₃₋₆ cycloalkyl group, an optionally substituted C₂₋₆ alkenyl group, an optionally substituted C₂₋₆ alkynyl group, an optionally substituted C₆₋₁₀ aryl group, an optionally substituted C₁₋₆ alkoxy group, an optionally substituted C₃₋₆ cycloalkoxy group, or an optionally substituted 4- to 6-membered heterocyclic group.

R⁴ is preferably

(1) a C₁₋₆ alkyl group (e.g., methyl, ethyl, propyl, isopropyl, butyl, isobutyl, tert-butyl, pentyl) optionally substituted by 1 to 3 substituents selected from a halogen atom (e.g., a fluorine atom), a hydroxy group, a C₃₋₆ cycloalkyl group (e.g., cyclopropyl), a C₁₋₆ alkoxy group (e.g., methoxy, ethoxy, isopropoxy), a C₁₋₆ alkoxy-carbonyl group (e.g., ethoxycarbonyl), and a 3- to 14-membered non-aromatic heterocyclyloxy group (e.g., tetrahydropyranyloxy), (2) a C₃₋₆ cycloalkyl group (e.g., cyclopropyl, cyclobutyl) optionally substituted by 1 to 3 C₁₋₆ alkyl groups (e.g., methyl), (3) a C₂₋₆ alkenyl group (e.g., ethenyl, propenyl, butenyl), (4) a C₂₋₆ alkynyl group (e.g., ethynyl, propynyl, butynyl, pentynyl, isopentynyl) optionally substituted by 1 to 3 C₃₋₆ cycloalkyl groups (e.g., cyclopropyl), (5) a C₆₋₁₀ aryl group (e.g., phenyl) optionally substituted by 1 to 3 substituents selected from a halogen atom (e.g., a fluorine atom), an optionally halogenated C₁₋₆ alkyl group (e.g., methyl, trifluoromethyl) and a C₁₋₆ alkoxy group (e.g., methoxy), (6) a C₁₋₆ alkoxy group (e.g., methoxy, ethoxy, propoxy, isopropoxy) optionally substituted by 1 to 3 C₃₋₆ cycloalkoxy groups (e.g., cyclopropyl), (7) a C₃₋₆ cycloalkoxy group (e.g., cyclobutoxy), or (8) a 4- to 6-membered heterocyclic group (e.g., oxetanyl, furyl, pyrazolyl, pyridyl) optionally substituted by 1 to 3 C₁₋₆ alkyl groups (e.g., methyl).

R⁴ is more preferably

(1) a C₁₋₆ alkyl group (e.g., methyl, ethyl, propyl), (2) a C₃₋₆ cycloalkyl group (e.g., cyclopropyl), (3) a C₂₋₆ alkenyl group (e.g., propenyl), (4) a C₂₋₆ alkynyl group (e.g., propynyl), (5) a C₆₋₁₀ aryl group (e.g., phenyl), or (6) a C₁₋₆ alkoxy group (e.g., ethoxy).

R⁴ is particularly preferably

(1) a C₁₋₆ alkyl group (e.g., ethyl), (2) a C₂₋₆ alkynyl group (e.g., propynyl), or (3) a C₁₋₆ alkoxy group (e.g., ethoxy).

Preferable compound is compound (I) wherein

R¹ is a C₁₋₆ alkyl group (e.g., methyl, ethyl), a mono- or di-C₁₋₆ alkylamino group (e.g., methylamino, dimethylamino), or a C₃₋₆ cycloalkyl group (e.g., cyclopropyl); R² is a hydrogen atom, a halogen atom (e.g., a fluorine atom, a bromine atom), a cyano group, a C₁₋₆ alkyl group (e.g., methyl), a C₃₋₆ cycloalkyl group (e.g., cyclopropyl), or a C₁₋₆ alkoxy group (e.g., methoxy); R³ is a hydrogen atom, a halogen atom (e.g., a fluorine atom, a bromine atom), a cyano group, a C₁₋₆ alkyl group optionally substituted by 1 to 3 hydroxy groups (e.g., methyl, ethyl, isopropyl), a C₃₋₆ cycloalkyl group (e.g., cyclopropyl), or a C₁₋₆ alkoxy group (e.g., methoxy); and

R⁴ is

(1) a C₁₋₆ alkyl group (e.g., methyl, ethyl, propyl, isopropyl, butyl, isobutyl, tert-butyl, pentyl) optionally substituted by 1 to 3 substituents selected from a halogen atom (e.g., a fluorine atom), a hydroxy group, a C₃₋₆ cycloalkyl group (e.g., cyclopropyl), a C₁₋₆ alkoxy group (e.g., methoxy, ethoxy, isopropoxy), a C₁₋₆ alkoxy-carbonyl group (e.g., ethoxycarbonyl), and a 3- to 14-membered non-aromatic heterocyclyloxy group (e.g., tetrahydropyranyloxy), (2) a C₃₋₆ cycloalkyl group (e.g., cyclopropyl, cyclobutyl) optionally substituted by 1 to 3 C₁₋₆ alkyl groups (e.g., methyl), (3) a C₂₋₆ alkenyl group (e.g., ethenyl, propenyl, butenyl), (4) a C₂₋₆ alkynyl group (e.g., ethynyl, propynyl, butynyl, pentynyl, isopentynyl) optionally substituted by 1 to 3 C₃₋₆ cycloalkyl groups (e.g., cyclopropyl), (5) a C₆₋₁₀ aryl group (e.g., phenyl) optionally substituted by 1 to 3 substituents selected from a halogen atom (e.g., a fluorine atom), an optionally halogenated C₁₋₆ alkyl group (e.g., methyl, trifluoromethyl) and a C₁₋₆ alkoxy group (e.g., methoxy), (6) a C₁₋₆ alkoxy group (e.g., methoxy, ethoxy, propoxy, isopropoxy) optionally substituted by 1 to 3 C₃₋₆ cycloalkoxy groups (e.g., cyclopropyl), (7) a C₃₋₆ cycloalkoxy group (e.g., cyclobutoxy), or (8) a 4- to 6-membered heterocyclic group (e.g., oxetanyl, furyl, pyrazolyl, pyridyl) optionally substituted by 1 to 3 C₁₋₆ alkyl groups (e.g., methyl).

More preferable compound is compound (I) wherein

R¹ is a C₁₋₆ alkyl group (e.g., methyl, ethyl), a mono- or di-C₁₋₆ alkylamino group (e.g., methylamino, dimethylamino), or a C₃₋₆ cycloalkyl group (e.g., cyclopropyl); R² is a hydrogen atom; R³ is a C₁₋₆ alkyl group (e.g., methyl); and

R⁴ is

(1) a C₁₋₆ alkyl group (e.g., methyl, ethyl, propyl), (2) a C₃₋₆ cycloalkyl group (e.g., cyclopropyl), (3) a C₂₋₆ alkenyl group (e.g., propenyl), (4) a C₂₋₆ alkynyl group (e.g., propynyl), (5) a C₆₋₁₀ aryl group (e.g., phenyl), or (6) a C₁₋₆ alkoxy group (e.g., ethoxy).

Particularly preferable compound is compound (I) wherein

R¹ is a C₁₋₆ alkyl group (e.g., methyl, ethyl); R² is a hydrogen atom; R³ is a C₁₋₆ alkyl group (e.g., methyl); and

R⁴ is

(1) a C₁₋₆ alkyl group (e.g., ethyl), (2) a C₂₋₆ alkynyl group (e.g., propynyl), or (3) a C₁₋₆ alkoxy group (e.g., ethoxy).

Specific examples of compound (I) include the compounds of Examples 1 to 149 mentioned below.

Among them, specific compound (I) is preferably

-   N-[7-methyl-4-({[(1s,4S)-4-methylcyclohexyl]oxy}methyl)-6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]methanesulfonamide     (Example 1) or a salt thereof, -   N-[7-methyl-6-oxo-4-({[(1s,4S)-4-phenylcyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]methanesulfonamide     (Example 6) or a salt thereof, -   N-[4-({[(1s,4S)-4-ethylcyclohexyl]oxy}methyl)-7-methyl-6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]methanesulfonamide     (Example 9) or a salt thereof, -   N-[7-methyl-6-oxo-4-({[(1r,4S)-4-propylcyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]cyclopropanesulfonamide     (Example 78) or a salt thereof, -   N-[7-methyl-6-oxo-4-({[(1s,4S)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]methanesulfonamide     (Example 109) or a salt thereof, -   N-{7-methyl-6-oxo-4-[({(1s,4S)-4-[(1Z)-prop-1-en-1-yl]cyclohexyl}oxy)methyl]-1,3,4,6-tetrahydro-2H-quinolizin-3-yl}methanesulfonamide     (Example 110) or a salt thereof, -   N-[4-({[(1s,4S)-4-cyclopropylcyclohexyl]oxy}methyl)-7-methyl-6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]methanesulfonamide     (Example 111) or a salt thereof, -   N-[4-({[(1s,4S)-4-cyclopropylcyclohexyl]oxy}methyl)-7-methyl-6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]ethanesulfonamide     (Example 112) or a salt thereof, -   N-[4-({[(1s,4S)-4-cyclopropylcyclohexyl]oxy}methyl)-7-methyl-6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]cyclopropanesulfonamide     (Example 113) or a salt thereof, -   N-[7-methyl-6-oxo-4-({[(1s,4S)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]ethanesulfonamide     (Example 121) or a salt thereof, -   N-[7-methyl-6-oxo-4-({[(1s,4S)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]cyclopropanesulfonamide     (Example 122) or a salt thereof, -   N-{7-methyl-6-oxo-4-[({(1s,4S)-4-[(1Z)-prop-1-en-1-yl]cyclohexyl}oxy)methyl]-1,3,4,6-tetrahydro-2H-quinolizin-3-yl}ethanesulfonamide     (Example 127) or a salt thereof, -   N-{7-methyl-6-oxo-4-[({(1s,4S)-4-[(1Z)-prop-1-en-1-yl]cyclohexyl}oxy)methyl]-1,3,4,6-tetrahydro-2H-quinolizin-3-yl}cyclopropanesulfonamide     (Example 128) or a salt thereof, -   N,N-dimethyl-N′-[7-methyl-6-oxo-4-({[(1s,4S)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]sulfuric     diamide (Example 144) or a salt thereof, -   N-[4-({[(1s,4S)-4-ethoxycyclohexyl]oxy}methyl)-7-methyl-6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]methanesulfonamide     (Examples 59, 145) or a salt thereof, -   N-[7-methyl-6-oxo-4-({[(1s,4S)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]methanesulfonamide     (Examples 109, 146) or a salt thereof, -   N-[7-methyl-6-oxo-4-({[(1s,4S)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]ethanesulfonamide     (Examples 121, 147) or a salt thereof, -   N-[-7-methyl-6-oxo-4-({[(1s,4S)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]cyclopropanesulfonamide     (Examples 122, 148) or a salt thereof, or -   N-methyl-N′-[7-methyl-6-oxo-4-({[(1s,4S)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]sulfuric     diamide (Example 149) or a salt thereof.

Specific compound (I) is particularly preferably

-   N-[4-({[(1s,4S)-4-ethylcyclohexyl]oxy}methyl)-7-methyl-6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]methanesulfonamide     (Example 9) or a salt thereof, -   N-[4-({[(1s,4S)-4-ethoxycyclohexyl]oxy}methyl)-7-methyl-6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]methanesulfonamide     (Examples 59, 145) or a salt thereof, or -   N-[7-methyl-6-oxo-4-({[(1s,4S)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]ethanesulfonamide     (Examples 121, 147) or a salt thereof.

As a salt of a compound represented by the formula (I), a pharmacologically acceptable salt is preferable, and examples of such salt include a salt with inorganic base, a salt with organic base, a salt with inorganic acid, a salt with organic acid, a salt with basic or acidic amino acid and the like.

Preferable examples of the salt with inorganic base include alkali metal salts such as sodium salt, potassium salt and the like, alkaline earth metal salts such as calcium salt, magnesium salt and the like, aluminum salt, ammonium salt and the like.

Is Preferable examples of the salt with organic base include salts with trimethylamine, triethylamine, pyridine, picoline, ethanolamine, diethanolamine, triethanolamine, tromethamine[tris(hydroxymethyl)methylamine], tert-butylamine, cyclohexylamine, benzylamine, dicyclohexylamine, N,N-dibenzylethylenediamine and the like.

Preferable examples of the salt with inorganic acid include salts with hydrochloric acid, hydrobromic acid, nitric acid, sulfuric acid, phosphoric acid and the like.

Preferable examples of the salt with organic acid include salts with formic acid, acetic acid, trifluoroacetic acid, phthalic acid, fumaric acid, oxalic acid, tartaric acid, maleic acid, citric acid, succinic acid, malic acid, methanesulfonic acid, benzenesulfonic acid, p-toluenesulfonic acid and the like.

Preferable examples of the salt with basic amino acid include salts with arginine, lysine, ornithine and the like. Preferable examples of the salt with acidic amino acid include salts with aspartic acid, glutamic acid and the like.

The production method of the compound of the present invention is explained below.

The raw material compound and reagent used and the compound obtained in each step in the following production method may be each in a form of a salt, and examples of such salt include those similar to the salts of the compound represented by the formula (I), and the like.

When the compound obtained in each step is a free form, it can be converted to the objective salt according to a method known per se. When the compound obtained in each step is a salt, it can be converted to the objective free form or the other salt according to a method known per se.

The compound obtained in each step can be used directly as the reaction mixture or as a crude product for the next reaction. Alternatively, the compound obtained in each step can be isolated and purified from a reaction mixture according to a method known per se, for example, a separation means such as concentration, crystallization, recrystallization, distillation, solvent extraction, fractional distillation, column chromatography and the like.

When the raw material compound and reagent used in each step are commercially available, the commercially available product can also be used directly.

In the reaction in each step, while the reaction time varies depending on the kind of the reagent and solvent to be used, it is generally 1 min-48 hr, preferably 10 min-8 hr, unless otherwise specified.

In the reaction in each step, while the reaction temperature varies depending on the kind of the reagent and solvent to be used, it is generally −78° C.-300° C., preferably −78° C.-150° C., unless otherwise specified.

In the reaction in each step, while the pressure varies depending on the kind of the reagent and solvent to be used, it is generally 1 atm-20 atm, preferably 1 atm-3 atm, unless otherwise specified.

Microwave synthesizer such as Initiator manufactured by Biotage and the like may be used for the reaction in each step. While the reaction temperature varies depending on the kind of the reagent and solvent to be used, it is generally room temperature-300° C., preferably 50° C.-250° C., unless otherwise specified. While the reaction time varies depending on the kind of the reagent and solvent to be used, it is generally 1 min-48 hr, preferably 1 min-8 hr, unless otherwise specified.

In the reaction in each step, the reagent is used in an amount of 0.5 equivalents-20 equivalents, preferably 0.8 equivalents-5 equivalents, relative to the substrate, unless otherwise specified. When the reagent is used as a catalyst, the reagent is used in an amount of 0.001 equivalent-1 equivalent, preferably 0.01 equivalent-0.2 equivalent, relative to the substrate. When the reagent is used as a reaction solvent, the reagent is used in a solvent amount.

Unless otherwise specified, the reaction in each step is carried out without solvent, or by dissolving or suspending the raw material compound in a suitable solvent. Examples of the solvent include those described in Examples and the following solvents.

alcohols: methanol, ethanol, tert-butyl alcohol, 2-methoxyethanol and the like; ethers: diethyl ether, diphenyl ether, tetrahydrofuran, 1,2-dimethoxyethane and the like; aromatic hydrocarbons: chlorobenzene, toluene, xylene and the like; saturated hydrocarbons: cyclohexane, hexane and the like; amides: N,N-dimethylformamide, N-methylpyrrolidone and the like; halogenated hydrocarbons: dichloromethane, carbon tetrachloride and the like; nitriles: acetonitrile and the like; sulfoxides: dimethyl sulfoxide and the like; aromatic organic bases: pyridine and the like; anhydrides: acetic anhydride and the like; organic acids: formic acid, acetic acid, trifluoroacetic acid and the like; inorganic acids: hydrochloric acid, sulfuric acid and the like; esters: ethyl acetate and the like; ketones: acetone, methyl ethyl ketone and the like; water.

The above-mentioned solvent can be used in a mixture of two or more kinds thereof in an appropriate ratio.

When a base is used for the reaction in each step, examples thereof include those described in Examples and the following bases.

inorganic bases: sodium hydroxide, magnesium hydroxide, sodium carbonate, calcium carbonate, sodium hydrogen carbonate and the like; organic bases: triethylamine, diethylamine, pyridine, 4-dimethylaminopyridine, N,N-dimethylaniline, 1,4-diazabicyclo[2.2.2]octane, 1,8-diazabicyclo[5.4.0]-7-undecene, imidazole, piperidine and the like; metal alkoxides: sodium ethoxide, potassium tert-butoxide and the like; alkali metal hydrides: sodium hydride and the like; metal amides: sodium amide, lithium diisopropylamide, lithium hexamethyldisilazide and the like; organic lithiums: n-butyllithium and the like.

When an acid or an acid catalyst is used for the reaction in each step, examples thereof include those described in Examples and the following acids and acid catalysts.

inorganic acids: hydrochloric acid, sulfuric acid, nitric acid, hydrobromic acid, phosphoric acid and the like; organic acids: acetic acid, trifluoroacetic acid, citric acid, p-toluenesulfonic acid, 10-camphorsulfonic acid and the like; Lewis acid: boron trifluoride diethyl ether complex, zinc iodide, anhydrous aluminum chloride, anhydrous zinc chloride, anhydrous iron chloride and the like.

Unless otherwise specified, the reaction in each step is carried out according to a method known per se, for example, the method described in Jikken Kagaku Kouza, 5th Edition, vol. 13-19 (the Chemical Society of Japan ed.); Shin Jikken Kagaku Kouza, vol. 14-15 (the Chemical Society of Japan ed.); Fine Organic Chemistry, Revised 2nd Edition (L. F. Tietze, Th. Eicher, Nankodo); Organic Name Reactions, the Reaction Mechanism and Essence, Revised Edition (Hideo Togo, Kodansha); ORGANIC SYNTHESES Collective Volume I-VII (John Wiley & Sons Inc.); Modern Organic Synthesis in the Laboratory A Collection of Standard Experimental Procedures (Jie Jack Li, OXFORD UNIVERSITY); Comprehensive Heterocyclic Chemistry III, Vol. 1-Vol. 14 (Elsevier Japan); Strategic Applications of Named Reactions in Organic Synthesis (translated by Kiyoshi Tomioka, Kagakudojin); Comprehensive Organic Transformations (VCH Publishers Inc.), 1989, or the like, or the method described in Examples.

In each step, the protection or deprotection reaction of a functional group is carried out according to a method known per se, for example, the method described in “Protective Groups in Organic Synthesis, 4th Ed”, Wiley-Interscience, Inc., 2007 (Theodora W. Greene, Peter G. M. Wuts); “Protecting Groups 3rd Ed.” Thieme, 2004 (P. J. Kocienski), or the like, or the method described in Examples.

Examples of the protecting group for a hydroxy group of an alcohol and the like and a phenolic hydroxy group include ether-type protecting groups such as methoxymethyl ether, benzyl ether, tert-butyldimethylsilyl ether, tetrahydropyranyl ether and the like; carboxylate ester-type protecting groups such as acetate ester and the like; sulfonate ester-type protecting groups such as methanesulfonate ester and the like; carbonate ester-type protecting groups such as tert-butylcarbonate and the like, and the like.

Examples of the protecting group for a carbonyl group of an aldehyde include acetal-type protecting groups such as dimethylacetal and the like; cyclic acetal-type protecting groups such as 1,3-dioxane and the like, and the like.

Examples of the protecting group for a carbonyl group of a ketone include ketal-type protecting groups such as dimethylketal and the like; cyclic ketal-type protecting groups such as 1,3-dioxane and the like; oxime-type protecting groups such as O-methyloxime and the like; hydrazone-type protecting groups such as N,N-dimethylhydrazone and the like, and the like.

Examples of the protecting group for a carboxyl group include ester-type protecting groups such as methyl ester and the like; amide-type protecting groups such as N,N-dimethylamide and the like, and the like.

Examples of the protecting group for a thiol include ether-type protecting groups such as benzyl thioether and the like; ester-type protecting groups such as thioacetate ester, thiocarbonate, thiocarbamate and the like, and the like.

Examples of the protecting group for an amino group and an aromatic heterocycle such as imidazole, pyrrole, indole and the like include carbamate-type protecting groups such as benzyl carbamate and the like; amide-type protecting groups such as acetamide and the like; alkyl amine-type protecting groups such as N-triphenylmethylamine and the like; sulfonamide-type protecting groups such as methanesulfonamide and the like, and the like.

The protecting groups can be removed according to a method known per se, for example, by employing a method using acid, base, ultraviolet rays, hydrazine, phenylhydrazine, sodium N-methyldithiocarbamate, tetrabutylammonium fluoride, palladium acetate, trialkylsilyl halide (e.g., trimethylsilyl iodide, trimethylsilyl bromide) and the like, a reduction method, and the like.

Is When reduction reaction is carried out in each step, examples of the reducing agent to be used include metal hydrides such as lithium aluminum hydride, sodium triacetoxyborohydride, sodium cyanoborohydride, diisobutylaluminum hydride (DIBAL-H), sodium borohydride, tetramethylammonium triacetoxyborohydride and the like; boranes such as borane tetrahydrofuran complex and the like; Raney nickel; Raney cobalt; hydrogen; formic acid; triethylsilane and the like. When carbon-carbon double bond or triple bond is reduced, a method using a catalyst such as palladium-carbon, Lindlar's catalyst and the like may be employed.

When oxidation reaction is carried out in each step, examples of the oxidizing agent to be used include peroxides such as m-chloroperbenzoic acid (mCPBA), hydrogen peroxide, tert-butylhydroperoxide and the like; perchlorates such as tetrabutylammonium perchlorate and the like; chlorates such as sodium chlorate and the like; chlorites such as sodium chlorite and the like; periodates such as sodium periodate and the like; hypervalent iodine reagents such as iodosylbenzene and the like; reagents containing manganese such as manganese dioxide, potassium permanganate and the like; leads such as lead tetraacetate and the like; reagents containing chromium such as pyridinium chlorochromate (PCC), pyridinium dichromate (PDC), Jones reagent and the like; halogen compounds such as N-bromosuccinimide (NBS) and the like; oxygen; ozone; sulfur trioxide-pyridine complex; osmium tetroxide; selenium dioxide; 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) and the like.

When radical cyclization reaction is carried out in each step, examples of the radical initiator to be used include azo compounds such as azobisisobutyronitrile (AIBN) and the like; water-soluble radical initiators such as 4-4′-azobis-4-cyanopentanoic acid (ACPA) and the like; triethylboron in the presence of air or oxygen; benzoyl peroxide and the like. Examples of the radical reagent to be used include tributylstannane, tristrimethylsilylsilane, 1,1,2,2-tetraphenyldisilane, diphenylsilane, samarium iodide and the like.

When Wittig reaction is carried out in each step, examples of the Wittig reagent to be used include alkylidene phosphoranes and the like. The alkylidene phosphoranes can be prepared according to a method known per se, for example, by reacting a phosphonium salt with a strong base.

When Horner-Emmons reaction is carried out in each step, examples of the reagent to be used include phosphonoacetates such as methyl dimethylphosphonoacetate, ethyl diethylphosphonoacetate and the like; and bases such as alkali metal hydrides, organic lithiums and the like.

When Friedel-Crafts reaction is carried out in each step, a combination of a Lewis acid and an acid chloride or a combination of a Lewis acid and an alkylating agent (e.g., an alkyl halide, an alcohol, an olefin etc.) is used as a reagent. Alternatively, an organic acid or an inorganic acid can also be used instead of a Lewis acid, and an anhydride such as acetic anhydride and the like can also be used instead of an acid chloride.

When aromatic nucleophilic substitution reaction is carried out in each step, a nucleophile (e.g., an amine, imidazole etc.) and a base (e.g., an organic base etc.) are used as a reagent.

When nucleophilic addition reaction by a carbo anion, nucleophilic 1,4-addition reaction (Michael addition reaction) by a carbo anion or nucleophilic substitution reaction by a carbo anion is carried out in each step, and examples of the base to be used for generation of the carbo anion include organic lithiums, metal alkoxides, inorganic bases, organic bases and the like.

When Grignard reaction is carried out in each step, examples of the Grignard reagent to be used include arylmagnesium halides such as phenylmagnesium bromide and the like; and alkylmagnesium halides such as methylmagnesium bromide and the like. The Grignard reagent can be prepared according to a method known per se, for example, by reacting an alkyl halide or an aryl halide with a metal magnesium in an ether or tetrahydrofuran as a solvent.

When Knoevenagel condensation reaction is carried out in each step, a compound having an activated methylene group with two electron withdrawing groups (e.g., malonic acid, diethyl malonate, malononitrile etc.) and a base (e.g., an organic base, a metal alkoxide, an inorganic base) are used as a reagent.

When Vilsmeier-Haack reaction is carried out in each step, phosphoryl chloride and an amide derivative (e.g., N,N-dimethylformamide etc.) are used as a reagent.

When azidation reaction of an alcohol, an alkyl halide or a sulfonate is carried out in each step, examples of the azidating agent to be used include diphenylphosphorylazide (DPPA), trimethylsilylazide, sodium azide and the like. For example, for the azidation reaction of an alcohol, a method using diphenylphosphorylazide and 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU), a method using trimethylsilylazide and a Lewis acid, and the like are employed.

When reductive amination reaction is carried out in each step, examples of the reducing agent to be used include sodium triacetoxyborohydride, sodium cyanoborohydride, hydrogen, formic acid and the like. When the substrate is an amine compound, examples of the carbonyl compound to be used include paraformaldehyde, aldehydes such as acetaldehyde and the like, and ketones such as cyclohexanone and the like. When the substrate is a carbonyl compound, examples of the amine to be used include ammonia, primary amines such as methylamine and the like; secondary amines such as dimethylamine and the like, and the like.

When Mitsunobu reaction is carried out in each step, an azodicarboxylate (e.g., diethyl azodicarboxylate (DEAD), diisopropyl azodicarboxylate (DIAD) etc.) and triphenylphosphine are used as a reagent.

When esterification reaction, amidation reaction or urea formation reaction is carried out in each step, examples of the reagent to be used include acyl halides such as acid chlorides, acid bromides and the like; activated carboxylic acids such as acid anhydrides, activated esters, sulfates and the like. Examples of the activating agent of the carboxylic acid include carbodiimide condensing agents such as 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (WSCD) and the like; triazine condensing agents such as 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride n-hydrate (DMT-MM) and the like; carbonate condensing agents such as 1,1-carbonyldiimidazole (CDI) and the like; diphenylphosphoryl azide (DPPA); benzotriazol-1-yloxy-trisdimethylaminophosphonium salt (BOP reagent); 2-chloro-1-methyl-pyridinium iodide (Mukaiyama reagent); thionyl chloride; lower alkyl haloformates such as ethyl chloroformate and the like; O-(7-azabenzotriazol-1-yl)-N,N,N′,N′-tetramethyluronium hexafluorophosphorate (HATU); sulfuric acid; combinations thereof and the like. When carbodiimide condensing agent is used, an additive such as 1-hydroxybenzotriazole (HOBt), N-hydroxysuccinimide (HOSu), dimethylaminopyridine (DMAP) and the like may be added to the reaction system.

When coupling reaction is carried out in each step, examples of the metal catalyst to be used include palladium compounds such as palladium(II) acetate, tetrakis(triphenylphosphine)palladium(0), dichlorobis(triphenylphosphine)palladium(II), dichlorobis(triethylphosphine)palladium(II), tris(dibenzylideneacetone)dipalladium(0), 1,1′-bis(diphenylphosphino)ferrocenepalladium(II) chloride and the like; nickel compounds such as tetrakis(triphenylphosphine)nickel(0) and the like; rhodium compounds such as tris(triphenylphosphine)rhodium(III) chloride and the like; cobalt compounds; copper compounds such as copper oxide, copper(I) iodide and the like; platinum compounds and the like. In addition, a base can be added to the reaction system, and examples thereof include inorganic bases and the like.

When thiocarbonylation reaction is carried out in each step, phosphorus pentasulfide is typically used as the thiocarbonylating agent. Alternatively, a reagent having a 1,3,2,4-dithiadiphosphetane-2,4-disulfide structure (e.g., 2,4-bis(4-methoxyphenyl)-1,3,2,4-dithiadiphosphetane-2,4-disulfide (Lawesson reagent) etc.) can also be used instead of phosphorus pentasulfide.

When Wohl-Ziegler reaction is carried out in each step, examples of the halogenating agent to be used include N-iodosuccinimide, N-bromosuccinimide (NBS), N-chlorosuccinimide (NCS), bromine, sulfuryl chloride and the like. In addition, the reaction can be accelerated by subjecting a radical initiator such as heat, light, benzoyl peroxide, azobisisobutyronitrile and the like to the reaction system reaction.

When halogenation reaction of a hydroxy group is carried out in each step, examples of the halogenating agent to be used include hydrohalic acids and acid halides of inorganic acids, specifically, hydrochloric acid, thionyl chloride, phosphorus oxychloride and the like for chlorination, 48% hydrobromic acid and the like for bromination. In addition, a method for producing an alkyl halide by reacting an alcohol with triphenylphosphine and carbon tetrachloride or carbon tetrabromide or the like can be employed. Alternatively, a method for producing an alkyl halide via two steps comprising converting an alcohol to the corresponding sulfonate, and then reacting the sulfonate with lithium bromide, lithium chloride or sodium iodide can also be employed.

When Arbuzov reaction is carried out in each step, examples of the reagent to be used include alkyl halides such as ethyl bromoacetate and the like; and phosphites such as triethyl phosphite, tri(isopropyl) phosphite and the like.

When sulfonate esterification reaction is carried out in each step, examples of the sulfonating agent to be used include methanesulfonyl chloride, p-toluenesulfonyl chloride, methanesulfonic anhydride, p-toluenesulfonic anhydride and the like.

When hydrolysis reaction is carried out in each step, an acid or a base is used as a reagent. For acid hydrolysis reaction of tert-butyl ester, formic acid, triethylsilane and the like may be added to reductively-trap tert-butyl cation which is by-produced.

When dehydration reaction is carried out in each step, examples of the dehydrating agent to be used include sulfuric acid, diphosphorus pentaoxide, phosphorus oxychloride, N,N′-dicyclohexylcarbodiimide, alumina, polyphosphoric acid and the like.

Compound (6) used in Scheme 2 mentioned below can be produced from compound (1) according to the method shown in the following Scheme 1. In the formulas, LG¹ and LG² are each independently a leaving group, R⁵ and R⁶ are each independently an optionally substituted C₁₋₆ alkyl group, and the other symbols are as defined above.

Examples of the “leaving group” for LG1 or LG² include a halogen atom, an optionally halogenated C₁₋₆ alkylsulfonyloxy group (e.g., methanesulfonyloxy, ethanesulfonyloxy, trifluoromethanesulfonyloxy), a C₆₋₁₄ arylsulfonyloxy group optionally substituted by a C₁₋₆ alkyl group (e.g., benzenesulfonyloxy, toluenesulfonyloxy), and the like.

Examples of the “optionally substituted C₁₋₆ alkyl group” for R⁵ or R⁶ include those in which the hydrocarbon group is a C₁₋₆ alkyl group, from among the “optionally substituted hydrocarbon group”.

Compound (1) may be used directly commercial product or can be produced according to a method known per se.

Compound (3) can be produced by subjecting compound (1) to a nucleophilic substitution reaction with compound (2). Compound (2) may be used directly commercial product or can be produced according to a method known per se. Examples of the base to be used include alkali metal hydrides, organic lithiums and the like.

Compound (5) can be produced by subjecting compound (3) to a coupling reaction with compound (4). Compound (4) may be used directly commercial product or can be produced according to a method known per se.

Compound (8) used in Scheme 3 mentioned below can be produced from compound (6) according to the method shown in the following Scheme 2. In the formulas, each symbol is as defined above.

Compound (7) can be produced by subjecting compound (6) to a condensation reaction in the presence of a base. Examples of the base to be used include inorganic bases, organic bases, alkali metal hydrides and the like.

Compound (8) can be produced by subjecting compound (7) to a decarboxylation reaction in the presence of an acid. Examples of the acid to be used include inorganic acids, organic acids and the like.

Compound (I) can be produced from compound (8) according to the method shown in the following Scheme 3. In the formulas, LG³ and LG⁴ are each independently a leaving group, and the other symbols are as defined above.

Examples of the “leaving group” for LG³ or LG⁴ include those exemplified in the “leaving group” for LG¹.

Compound (9) can be produced by subjecting compound (8) to an alkylation reaction with an aldehyde in the presence of a base. Examples of the aldehyde to be used include formaldehyde and the like. Examples of the base to be used include inorganic bases and the like.

Compound (12) can be produced by subjecting compound (10) to a nucleophilic substitution reaction with compound (11) in the presence of a base. Examples of the base to be used include inorganic bases and the like. An additive such as an organic boron compound and the like may be added to the reaction system. Examples of the organic boron compound to be used include 10H-phenoxaborinin-10-ol, 2,8-dimethyl-10H-phenoxaborinin-10-ol and the like. Compound (11) may be used directly commercial product or can be produced according to a method known per se.

Compound (14) can be produced by subjecting compound (13) to a reductive amination reaction. Examples of the reducing agent to be used include sodium triacetoxyborohydride, sodium cyanoborohydride, hydrogen, formic acid and the like. Examples of the amine to be used include ammonia and the like. A metal catalyst may be added. Examples of the catalyst to be used include iridium catalysts and the like. Alternatively, a method for producing compound (14) via two steps comprising converting compound (13) to the corresponding oxime, and then subjecting the oxime to a reduction reaction can also be employed. Examples of the amine to be used for the oxime formation include hydroxylamine, O-methylhydroxylamine and the like. Examples of the reducing agent to be used include boranes such as borane tetrahydrofuran complex and the like, sodium borohydride and the like. A metal catalyst may be added. Examples of the catalyst to be used include molybdenum(VI) trioxide and the like.

Compound (I) can be produced by subjecting compound (14) to a sulfonamidation reaction with compound (15) in the presence of a base. Examples of the base to be used include organic bases and the like. Compound (15) may be used directly commercial product or can be produced according to a method known per se.

In the thus-obtained compound (I), an intramolecular functional group can also be converted to an object functional group by a combination of chemical reactions known per se. Examples of the chemical reaction include oxidation reaction, reduction reaction, alkylation reaction, acylation reaction, ureation reaction, hydrolysis reaction, amination reaction, esterification reaction, aryl coupling reaction, deprotection reaction and the like.

In the above-mentioned production method, when a starting compound has an amino group, a carboxyl group, a hydroxy group, a carbonyl group or a mercapto group as a substituent, a protecting group generally used in the peptide chemistry may be introduced into these groups, and the object compound can be obtained by removing the protecting group as necessary after the reaction.

Compound (I) obtained by the above-mentioned production method can be isolated and purified by a known means, such as solvent extraction, liquid conversion, phase transfer, crystallization, recrystallization, chromatography and the like.

When compound (I) contains optical isomer, stereoisomer, regio isomer and rotamer, these compounds are also included in compound (I), and each can be obtained as a single product by a synthesis method or a separation method known per se. For example, when an optical isomer exists in compound (I), an optical isomer resolved from the compound is also encompassed in compound (I).

Here, an optical isomer can be produced by a method known per se.

Compound (I) may be a crystal.

A crystal of compound (I) (hereinafter sometimes to be abbreviated as the crystal of the present invention) can be produced by crystallizing compound (I), by applying a crystallization method known per se.

In the present specification, the melting point means a melting point measured, for example, by micro melting point apparatus (Yanako, MP-500D or Buchi, B-545), DSC (differential scanning calorimetry analysis) apparatus (METTLER TOLEDO, DSC1) and the like.

Generally, the melting point sometimes varies depending on the measurement device, measurement condition and the like. The crystal in the present specification may be a crystal showing a melting point different from the values described in the present specification as long as the difference is within a general error range.

The crystal of the present invention is superior in the physicochemical properties (e.g., melting point, solubility, stability) and biological properties (e.g., pharmacokinetics (absorbability, distribution, metabolism, excretion), efficacy expression), and is extremely useful as a medicament.

Compound (I) may be used as a prodrug. A prodrug of the compound (I) means a compound which is converted to the compound (I) of the present invention with a reaction due to an enzyme, a gastric acid, etc. under the physiological condition in the living body, that is, a compound which is converted to the compound (I) of the present invention with oxidation, reduction, hydrolysis, etc. according to an enzyme; a compound which is converted to the compound (I) of the present invention by hydrolysis etc. due to gastric acid, etc.

A prodrug of compound (I) may be a compound obtained by subjecting an amino group in compound (I) to an acylation, alkylation or phosphorylation (e.g., a compound obtained by subjecting an amino group in compound (I) to an eicosanoylation, alanylation, pentylaminocarbonylation, (5-methyl-2-oxo-1,3-dioxolen-4-yl)methoxycarbonylation, tetrahydrofuranylation, pyrrolidylmethylation, pivaloyloxymethylation and tert-butylation, etc.); a compound obtained by subjecting a hydroxy group in compound (I) to an acylation, alkylation, phosphorylation or boration (e.g., a compound obtained by subjecting a hydroxy group in compound (I) to an acetylation, palmitoylation, propanoylation, pivaloylation, succinylation, fumarylation, alanylation, dimethylaminomethylcarbonylation, etc.);

a compound obtained by subjecting a carboxyl group in compound (I) to an esterification or amidation (e.g., a compound obtained by subjecting a carboxyl group in compound (I) to an ethyl esterification, phenyl esterification, carboxymethyl esterification, dimethylaminomethyl esterification, pivaloyloxymethyl esterification, ethoxycarbonyloxyethyl esterification, phthalidyl esterification, (5-methyl-2-oxo-1,3-dioxolen-4-yl)methyl esterification, cyclohexyloxycarbonylethyl esterification and methylamidation, etc.) and the like. Any of these compounds can be produced from compound (I) by a method known per se.

A prodrug for compound (I) may also be one which is converted into compound (I) under a physiological condition, such as those described in IYAKUHIN no KAIHATSU (Development of Pharmaceuticals), Vol. 7, Design of Molecules, p. 163-198, Published by HIROKAWA SHOTEN (1990).

In the present specification, a prodrug may form a salt, and as such salt, those exemplified as a salt of the compound represented by the above-mentioned formula (I) can be mentioned.

Compound (I) may be labeled with an isotope (e.g., ³H, ¹³C, ¹⁴C, ¹⁸F, ³⁵S, ¹²⁵I) and the like.

Compound (I) labeled with or substituted by an isotope can be used, for example, as a tracer used for Positron Emission Tomography (PET) (PET tracer), and is useful in the field of medical diagnosis and the like.

Furthermore, compound (I) may be a hydrate or a non-hydrate, or a non-solvate (e.g., anhydride), or a solvate (e.g., hydrate).

Compound (I) also encompasses a deuterium conversion form wherein 1H is converted to ²H(D).

Furthermore, compound (I) may be a pharmaceutically acceptable cocrystal or cocrystal salt. The cocrystal or cocrystal salt means a crystalline substance constituted with two or more special solids at room temperature, each having different physical properties (e.g., structure, melting point, melting heat, hygroscopicity, solubility and stability). The cocrystal or cocrystal salt can be produced by a cocrystallization method known per se.

Compound (I) or a prodrug thereof (hereinafter sometimes to be simply abbreviated as the compound of the present invention) can be used as it is or in the form of a pharmaceutical composition (also referred to as a medicament) by mixing with a pharmacologically acceptable carrier etc. to mammals (e.g., human, mouse, rat, rabbit, dog, cat, bovine, horse, swine, monkey) as an agent for the prophylaxis or treatment of various diseases mentioned below.

As pharmacologically acceptable carriers, various organic or inorganic carrier substances conventionally used as preparation materials can be used. These are incorporated as excipient, lubricant, binder and disintegrant for solid preparations; or solvent, solubilizing agent, suspending agent, isotonicity agent, buffer and soothing agent for liquid preparations; and the like; and preparation additives such as preservative, antioxidant, colorant, sweetening agent and the like can be added as necessary.

Preferable examples of the excipient include lactose, sucrose, D-mannitol, D-sorbitol, starch, gelatinated starch, dextrin, crystalline cellulose, low-substituted hydroxypropylcellulose, sodium carboxymethylcellulose, gum arabic, pullulan, light anhydrous silicic acid, synthetic aluminum silicate and magnesium alumino metasilicate.

Preferable examples of the lubricant include magnesium stearate, calcium stearate, talc and colloidal silica.

Preferable examples of the binder include gelatinated starch, sucrose, gelatin, gum arabic, methylcellulose, carboxymethylcellulose, sodium carboxymethylcellulose, crystalline cellulose, sucrose, D-mannitol, trehalose, dextrin, pullulan, hydroxypropylcellulose, hydroxypropylmethylcellulose and polyvinylpyrrolidone.

Preferable examples of the disintegrant include lactose, sucrose, starch, carboxymethylcellulose, calcium carboxymethylcellulose, croscarmellose sodium, sodium carboxymethyl starch, light anhydrous silicic acid and low-substituted hydroxypropylcellulose.

Preferable examples of the solvent include water for injection, physiological brine, Ringer's solution, alcohol, propylene glycol, polyethylene glycol, sesame oil, corn oil, olive oil and cottonseed oil.

Preferable examples of the solubilizing agent include polyethylene glycol, propylene glycol, D-mannitol, trehalose, benzyl benzoate, ethanol, trisaminomethane, cholesterol, triethanolamine, sodium carbonate, sodium citrate, sodium salicylate and sodium acetate.

Preferable examples of the suspending agent include surfactants such as stearyltriethanolamine, sodium lauryl sulfate, lauryl aminopropionate, lecithin, benzalkonium chloride, benzethonium chloride, glycerol monostearate and the like; hydrophilic polymers such as poly(vinyl alcohol), polyvinylpyrrolidone, carboxymethylcellulose sodium, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose and the like, polysorbates; and polyoxyethylene hydrogenated castor oil.

Preferable examples of the isotonicity agent include sodium chloride, glycerol, D-mannitol, D-sorbitol and glucose.

Preferable examples of the buffer include buffers of phosphate, acetate, carbonate, citrate etc.

Preferable examples of the soothing agent include benzyl alcohol.

Preferable examples of the preservative include p-oxybenzoate esters, chlorobutanol, benzyl alcohol, phenethyl alcohol, dehydroacetic acid and sorbic acid.

Preferable examples of the antioxidant include sulfite salts and ascorbate salts.

Preferable examples of the colorant include aqueous food tar colors (e.g., food colors such as Food Color Red Nos. 2 and 3, Food Color Yellow Nos. 4 and 5, Food Color Blue Nos. 1 and 2 and the like food colors), water insoluble lake dyes (e.g., aluminum salt of the above-mentioned aqueous food tar color), natural dyes (e.g., β-carotene, chlorophyll, red iron oxide) and the like.

Preferable examples of the sweetening agent include saccharin sodium, dipotassium glycyrrhizinate, aspartame and stevia.

Examples of the dosage form of the above-mentioned pharmaceutical composition include oral preparations such as tablet (including sugar-coated tablet, film-coated tablet, sublingual tablet, orally disintegrating tablet, buccal tablet), capsule (including soft capsule, microcapsule), pill, granule, powder, troche, syrup, liquid, emulsion, suspension, aerosol, films (e.g., orally disintegrable films, oral mucosa-adhesive film) and the like; and parenteral agents such as injection (e.g., subcutaneous injection, intravenous injection, intramuscular injection, intraperitoneal injection, drip infusion), external preparation (e.g., transdermal absorption type preparation, ointment, lotion, adhesive preparation), suppository (e.g., rectal suppository, vaginal suppository), pellet, nasal preparation, pulmonary preparation (inhalant), eye drop and the like. The compound and medicament of the present invention can be respectively safely administered orally or parenterally (e.g., intrarectal, intravenous, intraarterial, intramuscular, subcutaneous, intraorgan, intranasal, intradermal, instillation, intracerebral, intravaginal, intraperitoneal, intratumoral, proximal tumor administrations, and administration to the lesion).

These preparations may be a release control preparation (e.g., sustained-release microcapsule) such as an immediate-release preparation, a sustained-release preparation and the like.

The pharmaceutical composition can be produced according to a method conventionally used in the field of pharmaceutical formulation, for example, the method described in the Japanese Pharmacopoeia, and the like.

While the content of the compound of the present invention in the pharmaceutical composition of the present invention varies depending on the dosage form, dose of the compound of the present invention and the like, it is, for example, about 0.1 to 100 wt %.

When an oral preparation is produced, coating may be applied where necessary for the purpose of taste masking, enteric solubility or sustainability.

Examples of the coating base used for coating include sugar coating base, water-soluble film coating base, enteric film coating base, and sustained-release film coating base.

As the sugar coating base, sucrose is used, and one or more kinds selected from talc, and the precipitated calcium carbonate, gelatin, gum arabic, pullulan, carnauba wax and the like may be further used in combination.

Examples of the water-soluble film coating base include cellulose polymers such as hydroxypropylcellulose, hydroxypropylmethylcellulose, hydroxyethylcellulose, methylhydroxyethylcellulose and the like; synthetic polymers such as polyvinyl acetal diethylaminoacetate, aminoalkylmethacrylate copolymer E [Eudragit E (trade name)], polyvinylpyrrolidone and the like; and polysaccharides such as pullulan and the like.

Examples of the enteric film coating base include cellulose polymers such as hydroxypropylmethylcellulose phthalate, hydroxypropylmethylcellulose acetate succinate, carboxymethylethylcellulose, cellulose acetate phthalate and the like; acrylic acid polymers such as methacrylic acid copolymer L [Eudragit L (trade name)], methacrylic acid copolymer LD [Eudragit L-30D-55 (trade name)], methacrylic acid copolymer S [Eudragit S (trade name)] and the like; and naturally-occurring substances such as shellac and the like.

Examples of the sustained-release film coating base include cellulose polymers such as ethylcellulose and the like; and acrylic acid polymers such as aminoalkylmethacrylate copolymer RS [Eudragit RS (trade name)], ethyl acrylate-methyl methacrylate copolymer suspension [Eudragit NE (trade name)] and the like.

Two or more kinds of the above-mentioned coating bases may be used in a mixture at an appropriate ratio. In addition, for example, light shielding agents such as titanium oxide, red ferric oxide and the like may also be used during coating.

Since the compound of the present invention shows low toxicity (e.g., acute toxicity, chronic toxicity, genetic toxicity, reproductive toxicity, cardiotoxicity, carcinogenicity) and less side effects, it can be used as a prophylactic or therapeutic agent, or diagnostic agent for various diseases in mammals (e.g., human, bovine, horse, dog, cat, monkey, mouse, rat).

The compound of the present invention has an excellent an orexin type 2 receptor agonist activity, and may treat, prevent or ameliorate the risk of various neurological and psychiatric diseases associated with an orexin type 2 receptor. The compound of the present invention is useful as an agent for the prophylaxis or treatment of various diseases such as narcolepsy, idiopathic hypersomnia, hypersomnia, sleep apnea syndrome, narcolepsy syndrome accompanied by narcolepsy-like symptoms, hypersomnia syndrome accompanied by daytime hypersomnia (e.g., Kleine Levin syndrome, major depression with hypersomnia, Lewy body dementia, Parkinson's disease, progressive supranuclear paralysis, Prader-Willi syndrome, Moebius syndrome, hypoventilation syndrome, Niemann-Pick disease type C, brain contusion, cerebral infarction, brain tumor, muscular dystrophy, multiple sclerosis, acute disseminated encephalomyelitis, Guillain-Barre syndrome, Rasmussen's encephalitis, Wernicke's encephalitis, limbic encephalitis, Hashimoto's encephalopathy), coma, loss of consciousness, obesity (e.g., malignant mastocytosis, exogenous obesity, hyperinsulinar obesity, hyperplasmic obesity, hypop hyseal adiposity, hypoplasmic obesity, hypothyroid obesity, hypothalamic obesity, symptomatic obesity, infantile obesity, upper body obesity, alimentary obesity, hypogonadal obesity, systemic mastocytosis, simple obesity, central obesity), insulin resistance syndrome, Alzheimer's disease, disturbance of consciousness such as coma and the like, side effects and complications due to anesthesia, sleep disturbance, sleep problem, insomnia, Intermittent sleep, nocturnal myoclonus, REM sleep interruption, jet lag, jet lag syndrome, sleep disorder of alternating worker, sleep disorder, night terror, depression, major depression, sleepwalking disease, enuresis, sleep disorder, Alzheimer's dusk, diseases associated with circadian rhythm, fibromyalgia, condition arising from decline in the quality of sleep, overeating, obsessive compulsive eating disorder, obesity-related disease, hypertension, diabetes, elevated plasma insulin concentration and insulin resistance, hyperlipidemia, hyperlipemia, endometrial cancer, breast cancer, prostate cancer, colorectal cancer, cancer, osteoarthritis, obstructive sleep apnea, cholelithiasis, gallstones, cardiac disease, abnormal heartbeat, arrhythmia, myocardial infarction, congestive cardiac failure, cardiac failure, coronary heart disease, cardiovascular disorder, sudden death, polycysticovarian disease, craniopharingioma, Froelich's syndrome, growth hormone deficient, normal mutant short stature, Turner's syndrome, children suffering from acute lymphoblastic leukemia, syndrome X, reproductive hormone abnormality, declining fertility, infertility, male gonadal function decline, sexual and reproductive dysfunction such as female male hirsutism, fetal defects associated with pregnant women obesity, gastrointestinal motility disorders such as obesity-related gastroesophageal reflux, obesity hypoventilation syndrome (Pickwick syndrome), respiratory diseases such as dyspnea, inflammation such as systemic inflammation of the vascular system, arteriosclerosis, hypercholesterolemia, hyperuricemia, lower back pain, gall bladder disease, gout, kidney cancer, risk of secondary outcomes of obesity such as lowering the risk of left ventricular hypertrophy, migraine pain, headache, neuropathic pain, Parkinson's disease, psychosis, schizophrenia, facial flushing, night sweats, diseases of the genital/urinary system, diseases related to sexual function or fertility, dysthymic disorder, bipolar disorder, bipolar I disorder, bipolar II disorder, cyclothymic disorder, acute stress disorder, agoraphobia, generalized anxiety disorder, obsessive disorder, panic attack, panic disorder, posttraumatic stress disorder, separation anxiety disorder, social phobia, anxiety disorder, acute neurological and psychiatric disorders such as cardiac bypass surgery and post-transplant cerebral deficit, stroke, ischemic stroke, cerebral ischemia, spinal cord trauma, head trauma, perinatal hypoxia, cardiac arrest, hypoglycemic nerve injury, Huntington's disease, amyotrophic lateral sclerosis, multiple sclerosis, eye damage, retinopathy, cognitive impairment, muscle spasm, tremor, epilepsy, disorders associated with muscle spasticity, delirium, amnestic disorder, age-related cognitive decline, schizoaffective disorder, delusional disorder, drug addiction, dyskinesia, chronic fatigue syndrome, fatigue, medication-induced Parkinsonism syndrome, Jill-do La Tourette's syndrome, chorea, myoclonus, tic, restless legs syndrome, dystonia, dyskinesia, attention deficit hyperactivity disorder (ADHD), behavior disorder, urinary incontinence, withdrawal symptoms, trigeminal neuralgia, hearing loss, tinnitus, nerve damage, retinopathy, macular degeneration, vomiting, cerebral edema, pain, bone pain, arthralgia, toothache, cataplexy, and traumatic brain injury.

Particularly, the compound of the present invention is useful as an agent for the prophylaxis or treatment of narcolepsy, idiopathic hypersomnia, hypersomnia, sleep apnea syndrome, narcolepsy syndrome accompanied by narcolepsy-like symptoms, hypersomnia syndrome accompanied by daytime hypersomnia (e.g., Parkinson's disease, Guillain-Barre syndrome and Kleine Levin syndrome), Alzheimer's disease, obesity, insulin resistance syndrome, cardiac failure, diseases related to bone loss, sepsis, disturbance of consciousness such as coma and the like, side effects and complications due to anesthesia, and the like, or anesthetic antagonist.

While the dose of the compound of the present invention varies depending on the subject of administration, administration route, target disease, symptom and the like, for example, when the compound of the present invention is administered orally or parenterally to an adult patient, its dose is for example, about 0.01 to 100 mg/kg body weight per dose, preferably 0.1 to 50 mg/kg body weight per dose and more preferably 0.5 to 20 mg/kg body weight per dose. This amount is desirably administered in one to 3 portions daily.

The compound of the present invention can be used in combination with other drugs (hereinafter to be abbreviated as concomitant drug).

By combining the compound of the present invention and a concomitant drug, a superior effect, for example,

(1) the dose can be reduced as compared to single administration of the compound of the present invention or a concomitant drug, (2) the drug to be combined with the compound of the present invention can be selected according to the condition of patients (mild case, severe case and the like), (3) the period of treatment can be set longer by selecting a concomitant drug having different action and mechanism from the compound of the present invention, (4) a sustained treatment effect can be designed by selecting a concomitant drug having different action and mechanism from the compound of the present invention, (5) a synergistic effect can be afforded by a combined use of the compound of the present invention and a concomitant drug, and the like, can be achieved.

In the present specification, the compound of the present invention and a concomitant drug used in combination are referred to as the “combination agent of the present invention”.

When using the combination agent of the present invention, the administration time of the compound of the present invention and the concomitant drug is not restricted, and the compound of the present invention or a pharmaceutical composition thereof, or the concomitant drug or a pharmaceutical composition thereof can be administered to an administration subject simultaneously, or may be administered at different times. The dosage of the concomitant drug may be determined according to the dose clinically used, and can be appropriately selected depending on an administration subject, administration route, disease, combination and the like.

The administration mode of the combination agent of the present invention and the concomitant drug is not particularly limited, and the compound of the present invention and the concomitant drug only need to be combined on administration. Examples of such administration mode include the following: (1) administration of a single preparation obtained by simultaneously processing the compound of the present invention and the concomitant drug, (2) simultaneous administration of two kinds of preparations of the compound of the present invention and the concomitant drug, which have been separately produced, by the same administration route, (3) administration of two kinds of preparations of the compound of the present invention and the concomitant drug, which have been separately produced, by the same administration route in a staggered manner, (4) simultaneous administration of two kinds of preparations of the compound of the present invention and the concomitant drug, which have been separately produced, by different administration routes, (5) administration of two kinds of preparations of the compound of the present invention and the concomitant drug, which have been separately produced, by different administration routes in a staggered manner (e.g., administration in the order of the compound of the present invention and the concomitant drug, or in the reverse order) and the like.

The dose of the concomitant drug can be appropriately determined based on the dose employed in clinical situations. The mixing ratio of the compound of the present invention and a concomitant drug can be appropriately determined depending on the administration subject, administration route, target disease, symptom, combination and the like.

For example, the content of the compound of the present invention in the combination agent of the present invention differs depending on the form of a preparation, and usually from about 0.01 to about 100 wt %, preferably from about 0.1 to about 50 wt %, further preferably from about 0.5 to about 20 wt %, based on the whole preparation.

The content of the concomitant drug in the combination agent of the present invention differs depending on the form of a preparation, and usually from about 0.01 to about 100 wt %, preferably from about 0.1 to about 50 wt %, further preferably from about 0.5 to about 20 wt %, based on the whole preparation.

The content of additives such as a carrier and the like in the combination agent of the present invention differs depending on the form of a preparation, and usually from about 1 to about 99.99 wt %, preferably from about 10 to about 90 wt %, based on the preparation.

Similar contents may be employed even when the compound of the present invention and a concomitant drug are separately formulated into preparations.

Examples of the concomitant drug include the followings. A therapeutic drug for narcolepsy (e.g., methylphenidate, amphetamine, pemoline, phenelzine, protriptyline, sodium oxybate, modafinil, caffeine), antiobesity drug (amphetamine, benzfetamine, bromocriptine, bupropion, diethylpropion, exenatide, fenfluramine, liothyronine, liraglutide, mazindol, methamphetamine, octreotide, octreotide, orlistat, phendimetrazine, phendimetrazine, phenmetrazine, phentermine, Qnexa (registered trade mark), phenylpropanolamine, pramlintide, propylhexedrine, recombinant leptin, sibutramine, topiramate, zimelidine, zonisamide, Lorcaserin, metformin), acetylcholine esterase inhibitor (e.g., donepezil, rivastigmine, galanthamine, zanapezil, idebenone, tacrine), antidementia agent (e.g., memantine), inhibitor of β amyloid protein production, secretion, accumulation, aggregation and/or deposition, β secretase inhibitor (e.g., 6-(4-biphenylyl)methoxy-2-[2-(N,N-dimethylamino)ethyl]tetralin, 6-(4-biphenylyl)methoxy-2-(N,N-dimethylamino)methyltetralin, 6-(4-biphenylyl)methoxy-2-(N,N-dipropylamino)methyltetralin, 2-(N,N-dimethylamino)methyl-6-(4′-methoxybiphenyl-4-yl)methoxytetralin, 6-(4-biphenylyl)methoxy-2-[2-(N,N-diethylamino)ethyl]tetralin, 2-[2-(N,N-dimethylamino)ethyl]-6-(4′-methylbiphenyl-4-yl)methoxytetralin, 2-[2-(N,N-dimethylamino)ethyl]-6-(4′-methoxybiphenyl-4-yl)methoxytetralin, 6-(2′,4′-dimethoxybiphenyl-4-yl)methoxy-2-[2-(N,N-dimethylamino)ethyl]tetralin, 6-[4-(1,3-benzodioxol-5-yl)phenyl]methoxy-2-[2-(N,N-dimethylamino)ethyl]tetralin, 6-(3′,4′-dimethoxybiphenyl-4-yl)methoxy-2-[2-(N,N-dimethylamino)ethyl]tetralin, an optically active form thereof, a salt thereof and a hydrate thereof, OM99-2 (WO01/00663)), y secretase inhibitor, β amyloid protein aggregation inhibitor (e.g., PTI-00703, ALZHEMED (NC-531), PPI-368 (National Publication of International Patent Application No. 11-514333), PPI-558 (National Publication of International Patent Application No. 2001-500852), SKF-74652 (Biochem. J. (1999), 340(1), 283-289)), β amyloid vaccine, β amyloid-degrading enzyme and the like, brain function enhancer (e.g., aniracetam, 25 nicergoline), therapeutic drug for Parkinson's disease [(e.g., dopamine receptor agonist (e.g., L-DOPA, bromocriptine, pergolide, talipexole, pramipexole, cabergoline, amantadine), monoamine oxidase enzyme (MAO) inhibitor (e.g., deprenyl, selegiline, remacemide, riluzole), anticholinergic agent (e.g., trihexyphenidyl, biperiden), COMT inhibitor (e.g., entacapone)], therapeutic drug for amyotrophic lateral sclerosis (e.g., riluzole etc., neurotrophic factor), therapeutic drug for abnormal behavior accompanying progress of dementia, wandering and the like (e.g., sedative, anti-anxiety drug), apoptosis inhibitor (e.g., CPI-1189, IDN-6556, CEP-1347), neuronal differentiation-regenerate promoter (e.g., leteprinim, xaliproden; SR-57746-A), SB-216763, Y-128, VX-853, prosaptide, 5,6-dimethoxy-2-[2,2,4,6,7-pentamethyl-3-(4-methylphenyl)-2,3-dihydro-1-benzofuran-5-yl]isoindoline, 5,6-dimethoxy-2-[3-(4-isopropylphenyl)-2,2,4,6,7-pentamethyl-2,3-dihydro-1-benzofuran-5-yl]isoindoline, 6-[3-(4-isopropylphenyl)-2,2,4,6,7-pentamethyl-2,3-dihydro-1-benzofuran-5-yl]-6,7-dihydro-5H-[1,3]dioxolo[4,5-f]isoindole and an optically active form, salt or hydrate thereof), non-steroidal antiinflammatory agents (meloxicam, tenoxicam, indomethacin, ibuprofen, celecoxib, rofecoxib, aspirin etc.), steroid drug (dexamethasone, hexestrol, cortisone acetate etc.), disease-modifying anti-rheumatic drug (DMARDs), anti-cytokine drug (e.g., TNF inhibitor, MAP kinase inhibitor), therapeutic agent for incontinence, frequent urination (e.g., flavoxate hydrochloride, oxybutynin hydrochloride, propiverine hydrochloride), phosphodiesterase inhibitor (e.g., sildenafil(citrate)), dopamine agonist (e.g., apomorphine), antiarrhythmic drugs (e.g., mexiletine), sex hormone or a derivative thereof (e.g., progesterone, estradiol, estradiol benzoate), therapeutic agent for osteoporosis (e.g., alfacalcidol, calcitriol, elcatonin, calcitonin salmon, estriol, ipriflavone, pamidronate disodium, alendronate sodium hydrate, incadronate disodium), parathyroid hormone (PTH), calcium receptor antagonists, therapeutic drug for insomnia (e.g., benzodiazepines medicament, non-benzodiazepines medicament, melatonin agonist, orexin receptor antagonists), therapeutic drug for schizophrenia (e.g., typical antipsychotic agents such as haloperidol and the like; atypical antipsychotic agents such as clozapine, olanzapine, risperidone, aripiprazole and the like; medicament acting on metabotropic glutamate receptor or ion channel conjugated-type glutamate receptor; phosphodiesterase inhibitor), benzodiazepines medicament (chlordiazepoxide, diazepam, potassium clorazepate, lorazepam, clonazepam, alprazolam etc.), L-type calcium channel inhibitor (pregabalin etc.), tricyclic or tetracyclic antidepressant (imipramine hydrochloride, amitriptyline hydrochloride, desipramine hydrochloride, clomipramine hydrochloride etc.), selective serotonin reuptake inhibitor (fluvoxamine maleate, fluoxetine hydrochloride, citalopram hydrobromide, sertraline hydrochloride, paroxetine hydrochloride, escitalopram oxalate etc.), serotonin-noradrenaline reuptake inhibitor (venlafaxine hydrochloride, duloxetine hydrochloride, desvenlafaxine hydrochloride etc.), noradrenaline reuptake inhibitor (reboxetine mesylate etc.), mirtazapine, trazodone hydrochloride, nefazodone hydrochloride, bupropion hydrochloride, setiptiline maleate, 5-HT_(1A) agonist, (buspirone hydrochloride, tandospirone citrate, osemozotan hydrochloride etc.), 5-HT_(2A) antagonist, 5-HT_(2A) inverse agonist, 5-HT₃ antagonist (cyamemazine etc.), heart non-selective β inhibitor (propranolol hydrochloride, oxprenolol hydrochloride etc.), histamine H₁ antagonist (hydroxyzine hydrochloride etc.), CRF antagonist, other antianxiety drug (meprobamate etc.), tachykinin antagonist (MK-869, saredutant etc.), medicament that acts on metabotropic glutamate receptor, CCK antagonist, β3 adrenaline antagonist (amibegron hydrochloride etc.), GAT-1 inhibitor (tiagabine hydrochloride etc.), N-type calcium channel inhibitor, carbonic anhydrase II inhibitor, NMDA glycine moiety agonist, NMDA antagonist (memantine etc.), peripheral benzodiazepine receptor agonist, vasopressin antagonist, vasopressin V1b antagonist, vasopressin V1a antagonist, phosphodiesterase inhibitor, opioid antagonist, opioid agonist, uridine, nicotinic acid receptor agonist, thyroid hormone (T3, T4), TSH, TRH, MAO inhibitor (phenelzine sulfate, tranylcypromine sulfate, moclobemide etc.), therapeutic drug for bipolar disorder (lithium carbonate, sodium valproate, lamotrigine, riluzole, felbamate etc.), cannabinoid CB1 antagonist (rimonabant etc.), FAAH inhibitor, sodium channel inhibitor, anti-ADHD drug (methylphenidate hydrochloride, methamphetamine hydrochloride etc.), therapeutic drug for alcoholism, therapeutic drug for autism, therapeutic drug for chronic fatigue syndrome, therapeutic drug for spasm, therapeutic drug for fibromyalgia syndrome, therapeutic drug for headache, therapeutic drug for quitting smoking, therapeutic drug for myasthenia gravis, therapeutic drug for cerebral infarction, therapeutic drug for mania, therapeutic drug for hypersomnia, therapeutic drug for pain, therapeutic drug for dysthymia, therapeutic drug for autonomic ataxia, therapeutic drug for male and female sexual dysfunction, therapeutic drug for migraine, therapeutic drug for pathological gambler, therapeutic drug for restless legs syndrome, therapeutic drug for substance addiction, therapeutic drug for alcohol-related syndrome, therapeutic drug for irritable bowel syndrome, therapeutic drug for lipid abnormality such as cholesterol-lowering drug (statin series (pravastatin sodium, atorvastatin, simvastatin, rosuvastatin etc.), fibrate (clofibrate etc.), squalene synthetase inhibitor), therapeutic drug for abnormal behavior or suppressant of dromomania due to dementia (sedatives, antianxiety drug etc.), therapeutic drug for diabetes, therapeutic agent for diabetic complications, therapeutic drug for hypertension, therapeutic drug for hypotension, diuretic, chemotherapeutic agent, immunotherapeutic agent, antithrombotic agent, anti-cancer agent and the like.

Two or more kinds of the above-mentioned concomitant drug may be used in a mixture at an appropriate ratio.

When the compound of the present invention is applied to each of the above-mentioned diseases, it can also be used in combination with biologics (e.g., antibody drug, nucleic acid or nucleic acid derivative, aptamer drug, vaccine preparation), or can be used in combination with a gene therapy method and the like, or can also be used in combination with a treatment in psychiatric field without using drugs.

Examples of the antibody drug and vaccine preparation include vaccine preparation against angiotensin II, vaccine preparation against CETP, CETP antibody, antibody against TNFα antibody and other cytokines, amyloid β vaccine preparation, vaccine for type 1 diabetes (e.g., DIAPEP-277 of Peptor), anti-HIV antibody and HIV vaccine preparation, as well as antibodies or vaccine preparations against cytokines, renin-angiotensin type enzymes and products thereof, antibodies or vaccine preparations against enzymes or proteins involved in blood lipid metabolism, antibodies or vaccines relating to enzymes and proteins involved in blood coagulation or fibrinolysis system, antibodies or vaccine preparations against proteins involved in sugar metabolism and insulin resistance, and the like. In addition, it can be used in combination with biologics relating to growth factors such as GH, IGF and the like.

Examples of the gene therapy method include a treatment method using gene relating to cytokine, renin-angiotensin type enzyme and product thereof, G protein, G protein conjugated receptor and phosphorylating enzyme thereof, a treatment method using a DNA decoy such as NFκB decoy and the like, a treatment method using antisense, a treatment method using a gene relating to an enzyme or protein involved in blood lipid metabolism (e.g., a gene relating to metabolism, excretion and absorption of cholesterol or triglyceride or HDL-cholesterol or blood phospholipid), a treatment method using a gene relating to an enzyme or protein involved in angiogenesis therapy for peripheral vascular obstruction and the like (e.g., growth factors such as HGF, VEGF etc.), a treatment method using a gene relating to a protein involved in glucose metabolism and insulin resistance, antisense against cytokines such as TNF etc., and the like.

Examples of the treatment method in the psychiatric field without using drug include modified electroconvulsive therapy, deep brain stimulation therapy, repetitive transcranial magnetic stimulation therapy, psychotherapy including cognitive behavioral therapy and the like.

The compound of the present invention can also be used in combination with various organ regeneration methods such as cardiac regeneration, renal regeneration, pancreatic regeneration, revascularization and the like, cell transplantation therapy utilizing bone marrow cells (bone marrow-derived mononuclear cell, myelogenic stem cell), or artificial organ utilizing tissue engineering (e.g., artificial blood vessel, cardiomyocyte sheet).

EXAMPLES

The present invention is explained in detail in the following by referring to Examples, Experimental Examples and Formulation Examples. However, the examples do not limit the present invention and the examples can be modified within the scope of the present invention.

The “room temperature” in the following Examples is generally about 10° C. to about 35° C. The ratio for mixed solvent is, unless otherwise specified, a volume mixing ratio and % means wt % unless otherwise specified.

The elution by column chromatography in the Examples was performed under the observation by TLC (Thin Layer Chromatography) unless otherwise specified. In the observation by TLC, 60 F₂₅₄ manufactured by Merck was used as a TLC plate, the solvent used as an elution solvent in column chromatography was used as a developing solvent, and UV detector was used for the detection. In silica gel column chromatography, the indication of NH means use of aminopropylsilane-bonded silica gel and the indication of DIOL means use of 3-(2,3-dihydroxypropoxy)propylsilane-bonded silica gel. In preparative HPLC (high performance liquid chromatography), the indication of C18 means use of octadecyl-bonded silica gel. The ratio for elution solvent is, unless otherwise specified, a volume mixing ratio.

For the analysis of ¹H NMR, ACD/SpecManager (trade name) software and the like were used. Peaks of a hydroxyl group, an amino group and the like, having very mild proton peak, are not sometimes described.

MS was measured by LC/MS. As the ionization method, ESI method, or APCI method was used. The data indicates actual measured value (found). While molecular ion peak is generally observed, a fragment ion is sometimes observed. In the case of a salt, a molecular ion peak or fragment ion peak of free form is generally observed.

In Example, 6-bromopyridin-2-ol may be present as a tautomer, 6-bromopyridin-2 (1H)-one.

In the following Examples, the following abbreviations are used.

MS: mass spectrum M: mol concentration N: normality CDCl₃: deuterochloroform DMSO-d₆: deuterodimethyl sulfoxide ¹H NMR: proton nuclear magnetic resonance LC/MS: liquid chromatograph mass spectrometer ESI: electron spray ionization APCI: atmospheric pressure chemical ionization TMSCl: trimethylsilyl chloride n-BuLi: n-butyllithium Dess-Martin Periodinane: 1,1,1-tris(acetyloxy)-1lambda˜5˜,2-benziodoxol-3 (1H)-one MeOH: methanol

NBS: N-bromosuccinimide

EtOH: ethanol TEA: triethylamine DIPEA: diisopropylethylamine THF: tetrahydrofuran DMSO: dimethyl sulfoxide TFA: trifluoroacetic acid

DMF: N,N-dimethylformamide

NH₃·H₂O: ammonium hydroxide SFC: supercritical fluid chromatography EtOAc: ethyl acetate DMAP: 4-dimethylaminopyridine

Example 1 rel-N-[(3S,4R)-7-methyl-4-({[(1s,4S)-4-methylcyclohexyl]oxy}methyl)-6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]methanesulfonamide with a Longer Retention Time by SFC (Column: CHIRALPAK AD-H, Mobile Phase: Carbon Dioxide/MeOH) A) 6-bromopyridin-2-ol

To a mixture of 2-bromo-6-methoxypyridine (4.64 g), sodium iodide (18.5 g) and acetonitrile (120 mL) was added TMSCl (13.4 g) at 0° C. The mixture was stirred at 50° C. for 5 hr. The mixture was poured into saturated aqueous sodium hydrogencarbonate solution at 0° C., and extracted with ethyl acetate. The organic layer was separated, washed with water and saturated brine, dried over anhydrous magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/hexane) to give the title compound (3.58 g).

MS: [M+H]⁺ 173.8.

B) methyl (6-bromo-2-oxopyridin-1(2H)-yl)acetate

To a mixture of 6-bromopyridin-2-ol (3.58 g) and THF (40 mL) was added lithium 2-methylpropan-2-olate (1.73 g) at room temperature. After 15 min, methyl bromoacetate (15.7 g) was added thereto, and the mixture was refluxed for 2 hr. The mixture was concentrated under reduced pressure, and the residue was diluted with ethyl acetate. The mixture was washed with water, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/hexane) to give the title compound (4.57 g).

MS: [M+H]⁺ 245.8.

C) ethyl (2E)-3-[1-(2-methoxy-2-oxoethyl)-6-oxo-1,6-dihydropyridin-2-yl]prop-2-enoate

A mixture of methyl (6-bromo-2-oxopyridin-1(2H)-yl)acetate (2.95 g), ethyl acrylate (6.00 g), DIPEA (4.65 g), palladium(II) acetate (0.215 g), tris(2-methylphenyl)phosphine (0.438 g) and acetonitrile (30 mL) was divided into two containers, and subjected to microwave irradiation at 150° C. for 1 hr. The mixture was concentrated under reduced pressure, and the residue was purified by silica gel column chromatography (ethyl acetate/hexane) to give the title compound (2.32 g).

MS: [M+H]⁺ 266.0.

D) ethyl (2E)-3-[5-bromo-1-(2-methoxy-2-oxoethyl)-6-oxo-1,6-dihydropyridin-2-yl]prop-2-enoate

To a mixture of ethyl (2E)-3-[1-(2-methoxy-2-oxoethyl)-6-oxo-1,6-dihydropyridin-2-yl]prop-2-enoate (1.42 g) and acetonitrile (5.5 mL) was added NBS (1.00 g) at room temperature. The mixture was stirred at room temperature for 3 hr, and concentrated under reduced pressure. The obtained solid was triturated in water, and the solid was collected by filtration. The obtained solid was washed with hexane to give the title compound (1.64 g).

MS: [M+H]⁺ 344.0.

E) ethyl (2E)-3-[1-(2-methoxy-2-oxoethyl)-5-methyl-6-oxo-1,6-dihydropyridin-2-yl]prop-2-enoate

To a mixture of trimethylboroxin (0.740 g) and toluene (8.0 mL) were added ethyl (2E)-3-[5-bromo-1-(2-methoxy-2-oxoethyl)-6-oxo-1,6-dihydropyridin-2-yl]prop-2-enoate (0.803 g), potassium carbonate (0.807 g), bis[4-(di-tert-butylphosphino)-N,N-dimethylaniline]dichloropalladium(II) (0.0431 g) and water (2.4 mL) at room temperature. The mixture was stirred under nitrogen atmosphere at 80° C. for 30 min. The mixture was cooled in ice bath, and the aqueous layer was separated, and extracted with ethyl acetate. The previous organic layer and the extracted organic layer were combined, dried over anhydrous sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/hexane) to give the title compound (0.578 g).

MS: [M+H]⁺ 280.1.

F) ethyl 3-[l-(2-methoxy-2-oxoethyl)-5-methyl-6-oxo-1,6-dihydropyridin-2-yl]propanoate

A mixture of ethyl (2E)-3-[1-(2-methoxy-2-oxoethyl)-5-methyl-6-oxo-1,6-dihydropyridin-2-yl]prop-2-enoate (26.1 g), 10% palladium on carbon (2.61 g), ethyl acetate (170 mL) and MeOH (85 mL) was stirred overnight under normal pressure of hydrogen atmosphere at room temperature, and then stirred for 1 day. The catalyst was removed by filtration, and the filtrate was concentrated under reduced pressure to give the title compound (26.2 g).

MS: [M+H]⁺ 282.2.

G) 4-(hydroxymethyl)-7-methyl-2H-quinolizine-3,6(1H,4H)-dione

To a mixture of ethyl 3-[1-(2-methoxy-2-oxoethyl)-5-25 methyl-6-oxo-1,6-dihydropyridin-2-yl]propanoate (11.1 g) and THF (200 mL) was added with stirring 28% sodium methanolate methanol solution (8.68 mL) at 0° C. After 30 min, the mixture was concentrated under reduced pressure. To the residue was added 6 M hydrochloric acid, and the mixture was refluxed for 60 min, and concentrated under reduced pressure. To the residue was added saturated brine, and the mixture was extracted with THF-ethyl acetate. The organic layer was separated, dried over anhydrous sodium sulfate, and concentrated under reduced pressure. To a mixture of the residue, THF (150 mL) and water (40 mL) was added sodium carbonate (3.35 g) at room temperature, and then, 37% aqueous formaldehyde solution (3.52 g) was added thereto. After 30 min, to the mixture was added dietary salt, and the mixture was extracted with ethyl acetate. The organic layer was separated, dried over anhydrous sodium sulfate, and concentrated under reduced pressure to give the title compound (8.88 g).

MS: [M+H]⁺ 208.1.

H) rac-7-hydroxy-6-(hydroxymethyl)-3-methyl-6,7,8,9-tetrahydro-4H-quinolizin-4-one

To a stirred MeOH (160 mL) was added sodium borohydride (1.85 g) at 0° C. After 5 min, to the mixture was added 4-(hydroxymethyl)-7-methyl-2H-quinolizine-3,6(1H,4H)-dione (6.77 g), and the mixture was warmed to room temperature. After 60 min, 2 M hydrochloric acid (24.5 mL) was added thereto, and the mixture was concentrated under reduced pressure. To the mixture was added methanol, and the mixture was concentrated under reduced pressure. The residue was purified by silica gel column chromatography (methanol/ethyl acetate) to give the title compound (5.31 g).

MS: [M+H]⁺ 210.1.

I) 10H-phenoxaborinin-10-ol

To a mixture of 1,1′-oxydibenzene (25.0 g) and THF (300 mL) was added 1.6 M n-BuLi hexane solution (190 mL) at −78° C. The mixture was stirred at −78° C. for 2 hr, and stirred overnight at room temperature. To the mixture was added trimethyl borate (22.9 g) at room temperature, and the mixture was stirred under nitrogen atmosphere at 70° C. for 2 hr. To the mixture was added 2M hydrochloric acid at 0° C., and the mixture was extracted with ethyl acetate. The organic layer was separated, washed with water and saturated brine, dried over anhydrous sodium sulfate, and concentrated under reduced pressure. The residue was crystallized from ethyl acetate/heptane, and the obtained solid was purified by silica gel column chromatography (ethyl acetate/hexane) to give the title compound (5.14 g).

¹H NMR (400 MHz, DMSO-d₆) δ 7.28 (2H, t, J=7.3 Hz), 7.43 (2H, d, J=8.3 Hz), 7.67 (2H, t, J=7.7 Hz), 8.14 (2H, d, J=7.5 Hz), 9.84 (1H, s).

J) (1r,4r)-4-methylcyclohexyl methanesulfonate

A mixture of (1r,4r)-4-methylcyclohexanol (1.38 g), TEA (3.03 g), methanesulfonic anhydride (5.22 g) and THF (40 mL) was stirred overnight at room temperature. The mixture was concentrated under reduced pressure, and the residue was purified by silica gel column chromatography (ethyl acetate/hexane) to give the title compound (2.16 g).

¹H NMR (400 MHz, CDCl₃) δ 0.90 (3H, d, J=6.5 Hz), 1.01-1.16 (2H, m), 1.31-1.45 (1H, m), 1.50-1.64 (2H, m), 1.73-1.84 (2H, m), 2.10-2.22 (2H, m), 2.99 (3H, s), 4.59 (1H, tt, J=11.2, 4.6 Hz).

K) 7-hydroxy-3-methyl-6-({[(1s,4s)-4-methylcyclohexyl]oxy}methyl)-6,7,8,9-tetrahydro-4H-quinolizin-4-one

A mixture of 7-hydroxy-6-(hydroxymethyl)-3-methyl-6,7,8,9-tetrahydro-4H-quinolizin-4-one (0.483 g), (1r,4r)-4-methylcyclohexyl methanesulfonate (2.16 g), 10H-phenoxaborinin-10-ol (0.181 g), cesium carbonate (3.76 g) and acetonitrile (15 mL) was stirred in a sealed tube container at 95° C. for 3 days. The mixture was filtered through Celite, and the filtrate was concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/hexane) to give the title compound (0.278 g).

MS: [M+H]⁺ 306.1.

L) 7-methyl-4-({[(1s,4s)-4-methylcyclohexyl]oxy}methyl)-2H-quinolizine-3,6(1H,4H)-dione

To a mixture of 7-hydroxy-3-methyl-6-({[(1s,4s)-4-methylcyclohexyl]oxy}methyl)-6,7,8,9-tetrahydro-4H-quinolizin-4-one (0.395 g) and acetonitrile (8 mL) was added Dess-Martin Periodinane (1.10 g) at 0° C. The mixture was stirred at room temperature for 1 hr. The mixture was filtered, and the so filtrate was concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/hexane) to give the title compound (0.198 g).

MS: [M+H]⁺ 304.1.

M) rac-(6R,7S)-7-amino-3-methyl-6-({[(1s,4S)-4-methylcyclohexyl]oxy}methyl)-6,7,8,9-tetrahydro-4H-quinolizin-4-one

A mixture of 7-methyl-4-({[(1s,4s)-4-methylcyclohexyl]oxy}methyl)-2H-quinolizine-3,6(1H,4H)-dione (0.198 g), hydroxylamine hydrochloride (0.181 g), sodium acetate (0.321 g) and EtOH (5 mL) was stirred under argon atmosphere at 90° C. for 1 hr. The mixture was concentrated under reduced pressure, and the residue was diluted with THF and saturated brine, and the mixture was extracted with ethyl acetate. The organic layer was separated, washed with saturated brine, dried over anhydrous magnesium sulfate, and concentrated under reduced pressure. To a mixture of the residue, molybdenum(VI) trioxide (0.125 g) and MeOH (5.8 mL) was added sodium borohydride (0.110 g) at room temperature. The mixture was stirred at room temperature for 1 hr, and sodium borohydride (0.110 g) was added thereto. The mixture was stirred at room temperature for additional 1 hr, and sodium borohydride (0.110 g) was added thereto. The mixture was neutralized with saturated aqueous sodium hydrogencarbonate solution at 0° C., and extracted with ethyl acetate. The organic layer was separated, washed with water and saturated brine, dried over anhydrous magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (NH, ethyl acetate/hexane) to give the title compound (0.0977 g).

MS: [M+H]⁺ 305.2.

N) rac-N-[(3S,4R)-7-methyl-4-({[(1s,4S)-4-methylcyclohexyl]oxy}methyl)-6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]methanesulfonamide

To a mixture of rac-(6R,7S)-7-amino-3-methyl-6-({[(1s,4S)-4-methylcyclohexyl]oxy}methyl)-6,7,8,9-tetrahydro-4H-quinolizin-4-one (74 mg), TEA (40 mg) and dichloromethane (4.0 mL) was added methanesulfonyl chloride (34 mg) at 0° C. The mixture was stirred under nitrogen atmosphere at room temperature for 2 hr, and concentrated under reduced pressure. The residue was purified by HPLC (C18, mobile phase: water/acetonitrile (containing 0.05% NH₃·H₂O)). The obtained fraction was concentrated under reduced pressure, and the residue was freeze-dried to give the title compound (44 mg).

MS: [M+H]⁺ 383.3.

O) rel-N-[(3S,4R)-7-methyl-4-({[(1s,4S)-4-methylcyclohexyl]oxy}methyl)-6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]methanesulfonamide with a Longer Retention Time by SFC (Column: CHIRALPAK AD-H, Mobile Phase: Carbon Dioxide/MeOH)

rac-N-[(3S,4R)-7-Methyl-4-({[(1s,4S)-4-methylcyclohexyl]oxy}methyl)-6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]methanesulfonamide (34 mg) was resolved by SFC (CHIRALPAK AD-H, 20 mmID×250 mmL, mobile phase: carbon dioxide/methanol=800/200 (v/v)) to give the title compound (13 mg).

¹H NMR (400 MHz, DMSO-d₆) δ 0.68-0.80 (4H, m), 0.97-1.34 (7H, m), 1.45-1.55 (1H, m), 1.63-1.75 (1H, m), 1.82-1.93 (1H, m), 1.96 (3H, s), 2.09-2.25 (1H, m), 2.90-3.05 (5H, m), 3.58-3.71 (2H, m), 3.75-3.84 (1H, m), 4.86-4.97 (1H, m), 5.97 (1H, d, J=7.0 Hz), 7.19 (1H, d, J=7.6 Hz), 7.49 (1H, d, J=6.2 Hz).

Example 6 rel-N-[(3S,4R)-7-methyl-6-oxo-4-({[(1s,4S)-4-phenylcyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]methanesulfonamide with a Shorter Retention Time by SFC (Column: CHIRALCEL OJ-H, Mobile Phase: Carbon Dioxide/MeOH) A) (1r,4r)-4-phenylcyclohexan-1-ol

To a mixture of 4-phenylcyclohexanone (50.0 g), THF (300 mL) and MeOH (30 mL) was added sodium borohydride (13.0 g) at 0° C. The mixture was stirred under nitrogen atmosphere at 15° C. for 16 hr. The mixture was poured into water, and extracted with ethyl acetate. The organic layer was separated, washed with saturated brine, dried over anhydrous sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/petroleum ether) to give the title compound (42.5 g).

¹H NMR (400 MHz, CDCl₃) δ 1.45-1.59 (4H, m), 1.92-1.98 (2H, m), 2.09-2.15 (2H, m), 2.47-2.57 (1H, m), 3.67-3.75 (1H, m), 7.18-7.34 (5H, m).

B) (1r,4r)-4-phenylcyclohexyl methanesulfonate

To a mixture of (1r,4r)-4-phenylcyclohexan-1-ol (1.0 g) and THF (20 mL) were added methanesulfonyl chloride (0.812 g) and TEA (0.861 g) at 0° C. The mixture was stirred overnight at room temperature. To the mixture was added saturated aqueous ammonium chloride solution, and the mixture was extracted with ethyl acetate. The organic layer was separated, washed with saturated aqueous ammonium chloride solution and saturated brine, dried over anhydrous sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/hexane) to give the title compound (1.35 g).

¹H NMR (300 MHz, CDCl₃) δ 1.51-1.83 (4H, m), 1.94-2.07 (2H, m), 2.22-2.35 (2H, m), 2.54 (1H, tt, J=11.8, 3.3 Hz), 3.04 (3H, s), 4.65-4.78 (1H, m), 7.12-7.36 (5H, m).

C) 7-hydroxy-3-methyl-6-({[(1s,4s)-4-phenylcyclohexyl]oxy}methyl)-6,7,8,9-tetrahydro-4H-quinolizin-4-one

A mixture of 7-hydroxy-6-(hydroxymethyl)-3-methyl-6,7,8,9-tetrahydro-4H-quinolizin-4-one (1.00 g), (1r,4r)-4-phenylcyclohexyl methanesulfonate (4.86 g), 10H-phenoxaborinin-10-ol (0.562 g), cesium carbonate (7.79 g) and acetonitrile (34 mL) was divided into two sealed tube containers, and stirred at 120° C. for 44 hr. The mixture was filtered through Celite, and the filtrate was concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/hexane) to give the title compound (0.995 g).

MS: [M+H]⁺ 368.2.

D) 7-methyl-4-({[(1s,4s)-4-phenylcyclohexyl]oxy}methyl)-2H-quinolizine-3,6(1H,4H)-dione

To a mixture of 7-hydroxy-3-methyl-6-({[(1s,4s)-4-phenylcyclohexyl]oxy}methyl)-6,7,8,9-tetrahydro-4H-quinolizin-4-one (0.995 g) and acetonitrile (10 mL) was added Dess-Martin Periodinane (2.30 g) at room temperature. The mixture was stirred at room temperature for 1 hr, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/hexane) to give the title compound (0.600 g).

MS: [M+H]⁺ 366.2.

E) rac-(6R,7S)-7-amino-3-methyl-6-({[(1s,4S)-4-phenylcyclohexyl]oxy}methyl)-6,7,8,9-tetrahydro-4H-quinolizin-4-one

A mixture of 7-methyl-4-({[(1s,4s)-4-phenylcyclohexyl]oxy}methyl)-2H-quinolizine-3,6(1H,4H)-dione (0.600 g), hydroxylamine hydrochloride (0.171 g), sodium acetate (0.269 g) and EtOH (16.3 mL) was stirred under nitrogen atmosphere at 70° C. for 1 hr. To the mixture were added saturated aqueous sodium hydrogencarbonate solution and dietary salt at room temperature, and the mixture was extracted with ethyl acetate. The organic layer was separated, dried over anhydrous sodium sulfate, and concentrated under reduced pressure. To a mixture of the residue, molybdenum(VI) trioxide (0.355 g) and MeOH (8.2 mL) was added sodium borohydride (0.186 g) at room temperature, and the mixture was stirred at room temperature for 30 min. To the mixture was added saturated aqueous sodium hydrogencarbonate solution under ice-cooling, and the mixture was warmed to room temperature. Dietary salt was added thereto, and the mixture was extracted with ethyl acetate-THF. The organic layer was separated, dried over anhydrous sodium sulfate, and concentrated under reduced pressure to give the title compound (0.530 g).

MS: [M+H]⁺ 367.2.

F) rac-N-[(3S,4R)-7-methyl-6-oxo-4-({[(1s,4S)-4-phenylcyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]methanesulfonamide

To a mixture of rac-(6R,7S)-7-amino-3-methyl-6-({[(1s,4S)-4-phenylcyclohexyl]oxy}methyl)-6,7,8,9-tetrahydro-4H-quinolizin-4-one (0.242 g), TEA (0.200 g) and THF (5.0 mL) was added methanesulfonic anhydride (0.262 g) at room temperature. The mixture was stirred at room temperature for 4 hr. To the mixture was added water, and the mixture was extracted with ethyl acetate. The organic layer was separated, washed with water and saturated brine, dried over anhydrous sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/hexane) to give the title compound (0.180 g).

MS: [M+H]⁺ 445.2.

G) rel-N-[(3S,4R)-7-methyl-6-oxo-4-({[(1s,4S)-4-phenylcyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]methanesulfonamide with a Shorter Retention Time by SFC (Column: CHIRALCEL OJ-H, Mobile Phase: Carbon Dioxide/MeOH)

rac-N-[(3S,4R)-7-Methyl-6-oxo-4-({[(1s,4S)-4-phenylcyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]methanesulfonamide (0.180 g) was resolved by SFC (CHIRALCEL OJ-H, 20 mmID×250 mmL, mobile phase: carbon dioxide/methanol=800/200 (v/v)) to give the title compound (0.082 g).

¹H NMR (400 MHz, CDCl₃) δ 1.55-1.80 (5H, m), 1.94-2.21 (6H, m), 2.24-2.35 (1H, m), 2.47-2.59 (1H, m), 2.90-3.13 (5H, m), 3.67-3.88 (5H, m), 5.30-5.37 (1H, m), 5.96 (1H, d, J=7.0 Hz), 6.27 (1H, d, J=8.7 Hz), 7.11-7.15 (1H, m), 7.16-7.34 (5H, m).

Example 9 rel-N-[(3S,4R)-4-({[(1s,4S)-4-ethylcyclohexyl]oxy}methyl)-7-methyl-6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]methanesulfonamide with a Shorter Retention Time by SFC (Column: CHIRALCEL OJ-H, Mobile Phase: Carbon Dioxide/MeOH) A) (1r,4r)-4-ethylcyclohexyl methanesulfonate

To a mixture of (1r,4r)-4-ethylcyclohexan-1-ol (1.79 g), TEA (2.83 g) and THF (70 mL) was added methanesulfonic anhydride (2.63 g) at 0° C. The mixture was stirred at room temperature for 1 hr. To the mixture was added saturated aqueous ammonium chloride solution at room temperature, and the mixture was extracted with ethyl acetate. The organic layer was separated, washed with saturated brine, dried over anhydrous sodium sulfate, and concentrated under reduced pressure to give the title compound (2.86 g).

¹H NMR (400 MHz, CDCl₃) δ 0.88 (3H, t, J=7.3 Hz), 0.94-1.29 (5H, m), 1.49-1.60 (2H, m), 1.78-1.93 (2H, m), 2.10-2.20 (2H, m), 3.00 (3H, s), 4.59 (1H, tt, J=11.2, 4.6 Hz).

B) 6-({[(1s,4s)-4-ethylcyclohexyl]oxy}methyl)-7-hydroxy-3-methyl-6,7,8,9-tetrahydro-4H-quinolizin-4-one

A mixture of 7-hydroxy-6-(hydroxymethyl)-3-methyl-6,7,8,9-tetrahydro-4H-quinolizin-4-one (0.500 g), (1r,4r)-4-ethylcyclohexyl methanesulfonate (2.96 g), cesium carbonate (4.67 g), 10H-phenoxaborinin-10-ol (0.375 g) and acetonitrile (20 mL) was stirred at 120° C. for 50 hr. The mixture was filtered through Celite, and the filtrate was concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/hexane) to give the title compound (0.760 g).

MS: [M+H]⁺ 320.2.

C) 4-({[(1s,4s)-4-ethylcyclohexyl]oxy}methyl)-7-methyl-2H-quinolizine-3,6(1H,4H)-dione

To a mixture of 6-({[(1s,4s)-4-ethylcyclohexyl]oxy}methyl)-7-hydroxy-3-methyl-6,7,8,9-tetrahydro-4H-quinolizin-4-one (0.500 g) and acetonitrile (15 mL) was added Dess-Martin Periodinane (1.33 g) at room temperature. The mixture was stirred at room temperature for 1 hr, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/hexane) to give the title compound (0.334 g).

MS: [M+H]⁺ 318.2.

D) rac-(6R,7S)-7-amino-6-({[(1s,4S)-4-25 ethylcyclohexyl]oxy}methyl)-3-methyl-6,7,8,9-tetrahydro-4H-quinolizin-4-one

A mixture of 4-({[(1s,4s)-4-ethylcyclohexyl]oxy}methyl)-7-methyl-2H-quinolizine-3,6(1H,4H)-dione (0.334 g), hydroxylamine hydrochloride (0.110 g), sodium acetate (0.173 g) and EtOH (10 mL) was stirred under nitrogen atmosphere at 70° C. for 1 hr. Saturated aqueous sodium hydrogencarbonate solution and dietary salt were added thereto at room temperature, and the mixture was extracted with ethyl acetate. The organic layer was separated, dried over anhydrous sodium sulfate, and concentrated under reduced pressure. To a mixture of the residue, molybdenum(VI) trioxide (0.227 g) and MeOH (10 mL) was added sodium borohydride (0.159 g) at room temperature. The mixture was stirred at room temperature for 30 min, and to the mixture was added sodium borohydride (0.119 g). The mixture was stirred at room temperature for 30 min, and cooled in ice bath, and saturated aqueous sodium hydrogencarbonate solution was added to the mixture. The mixture was allowed to warmed to room temperature, to the mixture was added dietary salt, and the mixture was extracted with ethyl acetate. The organic layer was separated, dried over anhydrous sodium sulfate, and concentrated under reduced pressure to give the title compound (0.263 g).

MS: [M+H]⁺ 319.2.

E) rel-N-[(3S,4R)-4-({[(1s,4S)-4-ethylcyclohexyl]oxy}methyl)-7-methyl-6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]methanesulfonamide with a Shorter Retention Time by SFC (Column: CHIRALCEL OJ-H, Mobile Phase: Carbon Dioxide/MeOH)

To a mixture of rac-(6R,7S)-7-amino-6-({[(1s,4S)-4-ethylcyclohexyl]oxy}methyl)-3-methyl-6,7,8,9-tetrahydro-4H-quinolizin-4-one (0.263 g), TEA (0.251 g) and THF (10 mL) was added methanesulfonic anhydride (0.288 mg) at room temperature. The mixture was stirred at room temperature for 2 hr, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/hexane), and resolved by SFC (CHIRALCEL OJ-H, 20 mmID×250 mmL, mobile phase: carbon dioxide/methanol=900/100 (v/v)) to give the title compound (0.062 g).

¹H NMR (400 MHz, CDCl₃) δ 0.87 (3H, t, J=7.2 Hz), 1.04-1.30 (5H, m), 1.33-1.61 (4H, m), 1.75-1.96 (2H, m), 2.03-2.17 (4H, m), 2.22-2.33 (1H, m), 2.89-3.10 (5H, m), 3.59-3.83 (4H, m), 5.30 (1H, dt, J=8.6, 4.3 Hz), 5.94 (1H, d, J=7.0 Hz), 6.32 (1H, d, J=8.7 Hz), 7.12 (1H, d, J=6.2 Hz).

Example 78 rel-N-[(3S,4R)-7-methyl-6-oxo-4-({[(1r,4S)-4-propylcyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]cyclopropanesulfonamide with a Shorter Retention Time by SFC (Column: CHIRALCEL OJ-H, Mobile Phase: Carbon Dioxide/MeOH) A) 2,8-dimethyl-10H-phenoxaborinin-10-ol

To a mixture of 1,1′-oxybis(4-methylbenzene) (47.5 g), N,N,N′,N′-tetramethylethane-1,2-diamine (69.6 g) and THF (480 mL) was added dropwise 1.6 M n-BuLi hexane solution (374 mL) at −78° C. The mixture was stirred at room temperature for 3 hr, and cooled to −78° C., and trimethylborate (62.2 g) was added dropwise to the mixture. The mixture was stirred overnight at room temperature. To the mixture was added saturated aqueous ammonium chloride solution (500 mL) at 0° C., and the mixture was stirred at room temperature for 5 hr. The aqueous layer was separated, and extracted with ethyl acetate. The organic layers were combined, dried over anhydrous magnesium sulfate, and concentrated under reduced pressure. To the residue were added ethyl acetate (20 mL) and hexane (200 mL), and the solid was collected by filtration. The obtained solid was washed with hexane, and suspended in ethyl acetate. The solid was collected by filtration, and washed with ethyl acetate to give the title compound (16.8 g).

MS: [M−H]⁻223.1.

B) 7-hydroxy-3-methyl-6-({[(1r,4s)-4-propylcyclohexyl]oxy}methyl)-6,7,8,9-tetrahydro-4H-quinolizin-4-one

To a stirred mixture of 4-propylcyclohexanone (32.0 g), THF (150 mL) and MeOH (20 mL) was added sodium borohydride (4.32 g) at 0° C. The mixture was stirred for 15 min, to the mixture was added water, and the mixture was concentrated under reduced pressure. The residue was diluted with ethyl acetate, and the mixture was washed with water and saturated brine, dried over anhydrous sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/hexane) to give (1s,4r)-4-propylcyclohexan-1-ol (19.0 g). To a stirred mixture of (1s,4r)-4-propylcyclohexan-1-ol (19.0 g), TEA (22 g) and THF (200 mL) was added methanesulfonic anhydride (29.1 g) at 0° C. The mixture was stirred for 30 min, to the mixture was added saturated aqueous sodium hydrogencarbonate solution, and the mixture was concentrated under reduced pressure. To the residue were added water and ethyl acetate. The organic layer was separated, washed with saturated brine, dried over anhydrous sodium sulfate, and concentrated under reduced pressure. To the residue were added hexane and seed crystals of (1s,4r)-4-propylcyclohexyl methanesulfonate, and the solid was collected by filtration. The solid was washed with hexane to give (1s,4r)-4-propylcyclohexyl methanesulfonate. A mixture of the obtained (1s,4r)-4-propylcyclohexyl methanesulfonate, 7-hydroxy-6-(hydroxymethyl)-3-methyl-6,7,8,9-tetrahydro-4H-quinolizin-4-one (4.65 g), 2,8-dimethyl-10H-phenoxaborinin-10-ol (5.98 g), cesium carbonate (43.5 g) and acetonitrile (150 mL) was stirred at 80° C. for 72 hr, and the mixture was concentrated under reduced pressure. The residue was diluted with ethyl acetate, and the mixture was washed with water, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/hexane) to give the title compound (2.30 g).

MS: [M+H]⁺ 334.3.

C) 7-methyl-4-({[(1r,4s)-4-propylcyclohexyl]oxy}methyl)-2H-quinolizine-3,6(1H,4H)-dione

To a mixture of 7-hydroxy-3-methyl-6-({[(1r,4s)-4-propylcyclohexyl]oxy}methyl)-6,7,8,9-tetrahydro-4H-quinolizin-4-one (2.30 g) and acetonitrile (69 mL) was added Dess-Martin Periodinane (3.51 g) at room temperature. The mixture was stirred at room temperature for 1 hr, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/hexane) to give the title compound (2.11 g).

MS: [M+H]⁺ 332.2.

D) rac-N-[(3S,4R)-7-methyl-6-oxo-4-({[(1r,4S)-4-propylcyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]cyclopropanesulfonamide

A mixture of 7-methyl-4-({[(1r,4s)-4-propylcyclohexyl]oxy}methyl)-2H-quinolizine-3,6(1H,4H)-dione (69.9 mg), ammonium formate (133 mg), chloro[4-(dimethylamino)-2-pyridinecarboxamidato] (pentamethylcyclopentadienyl)iridium(III) (1.1 mg) and MeOH (1 mL) was stirred at 70° C. for 1 hr, and concentrated under reduced pressure. The residue was diluted with ethyl acetate, and the mixture was washed twice with saturated aqueous sodium hydrogencarbonate solution, and concentrated under reduced pressure. The residue was diluted with THF (1 mL), the mixture was stirred, and TEA (64 mg) and cyclopropanesulfonyl chloride (53 mg) were added thereto at 0° C. The mixture was stirred for 15 min, to the mixture was added saturated aqueous sodium hydrogencarbonate solution, and the mixture was concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/hexane) to give the title compound (53.4 mg).

MS: [M+H]⁺ 437.3.

E) rel-N-[(3S,4R)-7-methyl-6-oxo-4-({[(1r,4S)-4-propylcyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]cyclopropanesulfonamide with a Shorter Retention Time by SFC (Column: CHIRALCEL OJ-H, Mobile Phase: Carbon Dioxide/MeOH)

rac-N-[(3S,4R)-7-Methyl-6-oxo-4-({[(1r,4S)-4-propylcyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]cyclopropanesulfonamide (52.6 mg) was resolved by SFC (column: CHIRALCEL OJ-H, 20 mmID×250 mmL, mobile phase: carbon dioxide/methanol=900/100) to give the title compound (22.4 mg).

¹H NMR (300 MHz, CDCl₃) δ 0.88 (3H, t, J=7.1 Hz), 0.99-1.59 (15H, m), 1.74-1.95 (2H, m), 2.06-2.18 (4H, m), 2.20-2.34 (1H, m), 2.42-2.60 (1H, m), 2.84-3.17 (2H, m), 3.56-3.90 (4H, m), 5.37-5.48 (1H, m), 5.91-5.98 (1H, m), 6.27 (1H, d, J=8.7 Hz), 7.12 (1H, dd, J=7.0, 1.0 Hz).

Example 109 rac-N-[(3S,4R)-7-methyl-6-oxo-4-({[(1s,4S)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]methanesulfonamide A) ethyl (1r,4r)-4-{[tert-butyl(diphenyl)silyl]oxy}cyclohexane-1-carboxylate

A mixture of ethyl trans-4-hydroxycyclohexanecarboxylate (25 g), tert-butyl(chloro)diphenylsilane (41.9 g), 1H-imidazole (10.9 g) and DMF (300 mL) was stirred overnight at room temperature. To the mixture was added 0.1 M hydrochloric acid at 0° C., and the mixture was extracted with ethyl acetate. The organic layer was separated, washed with water and saturated brine, dried over anhydrous magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/hexane) to give the title compound (54.8 g).

¹H NMR (400 MHz, CDCl₃) δ 1.05 (9H, s), 1.17-1.23 (3H, m), 1.25-1.45 (4H, m), 1.25-1.45 (4H, m), 1.80-1.95 (4H, m), 2.14-2.26 (1H, m), 3.53-3.69 (1H, m), 3.97-4.18 (2H, m), 7.33-7.45 (6H, m), 7.62-7.69 (4H, m).

B) [(1r,4r)-4-{[tert-butyl(diphenyl)silyl]oxy}cyclohexyl]methanol

To a stirred mixture of lithium aluminium hydride (9.98 g) and THF (100 mL) was added a mixture of ethyl (1r,4r)-4-{[tert-butyl(diphenyl)silyl]oxy}cyclohexane-1-carboxylate (108 g) and THF (100 mL) under ice bath. The mixture was stirred at 0° C. for 30 min, and water (5 mL) and 1 M aqueous sodium hydroxide solution (1 mL) were successively added thereto, and so the mixture was stirred for 30 min. Water (3 mL) was added again thereto, and the mixture was stirred at room temperature for 30 min. The mixture was filtered through Celite, and the filtrate was concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/hexane) to give the title compound (90 g).

¹H NMR (400 MHz, CDCl₃) δ 0.69-0.92 (2H, m), 1.05 (9H, s), 1.22-1.28 (1H, m), 1.31-1.47 (3H, m), 1.61-1.77 (2H, m), 1.80-1.91 (2H, m), 3.28-3.40 (2H, m), 3.51-3.62 (1H, m), 7.30-7.48 (6H, m), 7.58-7.76 (4H, m).

C) (1r,4r)-4-{[tert-butyl(diphenyl)silyl]oxy}cyclohexane-1-carbaldehyde

To a stirred mixture of [(1r,4r)-4-{[tert-butyl(diphenyl)silyl]oxy}cyclohexyl]methanol (10 g), TEA (8.24 g) and DMSO (70 mL) was added sulfur trioxide pyridine complex (13.0 g) little by little. The mixture was stirred at room temperature for 3 hr, and the mixture was poured into water, extracted with diethyl ether. The organic layer was separated, washed with 1M hydrochloric acid and saturated brine, dried over anhydrous sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/hexane) to give the title compound (8.79 g).

¹H NMR (300 MHz, CDCl₃) δ 1.00-1.11 (9H, m), 1.14-1.31 (2H, m), 1.34-1.53 (2H, m), 1.78-1.99 (4H, m), 2.12-2.25 (1H, m), 3.53-3.69 (1H, m), 7.32-7.49 (6H, m), 7.63-7.73 (4H, m), 9.54-9.61 (1H, m).

D) (1r,4r)-4-(prop-1-yn-1-yl)cyclohexyl methanesulfonate

A mixture of (1r,4r)-4-{[tert-butyl(diphenyl)silyl]oxy}cyclohexane-1-carbaldehyde (1 g), dimethyl (1-diazo-2-oxopropyl)phosphonate (0.495 mL), potassium carbonate (0.754 g) and MeOH (10 mL) was stirred at room temperature for 2 hr. Dimethyl (1-diazo-2-oxopropyl)phosphonate (0.1 mL) was added to the mixture, and the mixture was stirred for 30 min. The mixture was poured into water, and extracted with diethyl ether. The organic layer was separated, washed with saturated brine, dried over anhydrous magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/hexane) to give tert-butyl{[(1r,4r)-4-ethynylcyclohexyl]oxy}diphenylsilane (798 mg). To a stirred mixture of tert-butyl{[(1r,4r)-4-ethynylcyclohexyl]oxy}diphenylsilane (1.27 g) and THF (15 ml) was added 1.6 M n-BuLi hexane solution (2.85 mL) at −78° C. The mixture was stirred for 15 min, and to the mixture was added iodomethane (795 mg). The mixture was warmed to room temperature, diluted with ethyl acetate, washed with water and saturated brine, dried over anhydrous sodium sulfate, and concentrated under reduced pressure. The residue was diluted with THF (7 mL), and 1 M tetrabutylammonium fluoride THF solution (7.0 mL) was added thereto, and the mixture was stirred overnight at room temperature. The mixture was concentrated under reduced pressure, the residue was diluted with water, and the mixture was extracted with ethyl acetate. The organic layer was washed with saturated brine, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/hexane) to give (1r,4r)-4-(prop-1-yn-1-yl)cyclohexan-1-ol (305 mg). To a stirred mixture of (1r,4r)-4-(prop-1-yn-1-yl)cyclohexan-1-ol (305 mg), TEA (357 mg) and THF (6.6 mL) was added methanesulfonic anhydride (481 mg) at 0° C. The mixture was stirred for 30 min, to the mixture was added water, and the mixture was concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/hexane) to give the title compound (437 mg).

¹H NMR (300 MHz, CDCl₃) δ1.43-1.54 (2H, m), 1.58-1.73 (2H, m), 1.78 (3H, d, J=2.3 Hz), 1.90-2.02 (2H, m), 2.07-2.18 (2H, m), 2.30-2.43 (1H, m), 3.00 (3H, s), 4.64-4.77 (1H, m).

E) 7-hydroxy-3-methyl-6-({[(1s,4s)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-6,7,8,9-tetrahydro-4H-quinolizin-4-one

A mixture of 7-hydroxy-6-(hydroxymethyl)-3-methyl-6,7,8,9-tetrahydro-4H-quinolizin-4-one (168 mg), (1r,4r)-4-(prop-1-yn-1-yl)cyclohexyl methanesulfonate (435 mg), 2,8-dimethyl-10H-phenoxaborinin-10-ol (216 mg), cesium carbonate (655 mg) and acetonitrile (3.7 mL) was stirred at 80° C. for 72 hr. The mixture was concentrated under reduced pressure, and the residue was diluted with ethyl acetate. The organic layer was washed with water, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/hexane) to give the title compound (105 mg).

MS: [M+H]⁺ 330.2.

F) 7-methyl-4-({[(1s,4s)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-2H-quinolizine-3,6(1H,4H)-dione

To a mixture of 7-hydroxy-3-methyl-6-({[(1s,4s)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-6,7,8,9-tetrahydro-4H-25 quinolizin-4-one (105 mg) and acetonitrile (3.2 mL) was added Dess-Martin Periodinane (162 mg) at room temperature. The mixture was stirred at room temperature for 1 hr, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/hexane) to give the title compound (98.8 mg).

MS: [M+H]⁺ 328.2.

G) 7-(hydroxyimino)-3-methyl-6-({[(1s,4s)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-6,7,8,9-tetrahydro-4H-quinolizin-4-one

A mixture of 7-methyl-4-({[(1s,4s)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-2H-quinolizine-3,6(1H,4H)-dione (98.8 mg), sodium acetate (49.5 mg), hydroxylamine hydrochloride (31.5 mg) and EtOH (1.5 mL) was stirred at room temperature for 1 hr. The mixture was concentrated under reduced pressure, and the residue was purified by silica gel column chromatography (ethyl acetate/hexane) to give the title compound (103 mg).

MS: [M+H]⁺ 343.2.

H) rac-(6R,7S)-7-amino-3-methyl-6-({[(1s,4S)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-6,7,8,9-tetrahydro-4H-quinolizin-4-one

To a stirred mixture of 7-(hydroxyimino)-3-methyl-6-({[(1s,4s)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-6,7,8,9-tetrahydro-4H-quinolizin-4-one (103 mg) and MeOH (3 ml) were added molybdenum(VI) trioxide (87 mg) and sodium borohydride (68.3 mg) at room temperature. The mixture was stirred for 15 min, and cooled to 0° C., saturated aqueous sodium hydrogencarbonate solution was added thereto, and the mixture was extracted with ethyl acetate. The organic layer was separated, washed with saturated brine, and concentrated under reduced pressure to give the title compound (84.5 mg).

MS: [M+H]⁺ 329.3.

I) rac-N-[(3S,4R)-7-methyl-6-oxo-4-({[(1s,4S)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]methanesulfonamide

To a stirred mixture of rac-(6R,7S)-7-amino-3-methyl-6-({[(1s,4S)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-6,7,8,9-tetrahydro-4H-quinolizin-4-one (42.0 mg) and THF (1 mL) were added TEA (26 mg) and methanesulfonic anhydride (29.0 mg) at room temperature. The mixture was stirred for 15 min, to the mixture was added water, and the mixture was concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/hexane) to give the title compound (20.5 mg).

¹H NMR (300 MHz, CDCl₃) δ 1.57-1.88 (11H, m), 2.05-2.18 (4H, m), 2.22-2.35 (1H, m), 2.42-2.53 (1H, m), 2.85-3.12 (5H, m), 3.38-3.50 (1H, m), 3.66-3.88 (3H, m), 5.23-5.37 (1H, m), 5.91-6.01 (1H, m), 6.14-6.27 (1H, m), 7.13 (1H, dd, J=7.0, 1.1 Hz).

Example 110 rac-N-{(3S,4R)-7-methyl-6-oxo-4-[({(1s,4S)-4-[(1Z)-prop-1-en-1-yl]cyclohexyl}oxy)methyl]-1,3,4,6-tetrahydro-2H-quinolizin-3-yl}methanesulfonamide

A mixture of rac-N-[(3S,4R)-7-methyl-6-oxo-4-({[(1s,4S)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]methanesulfonamide (16.1 mg), palladium-polyethylene imine (trade name) (1.6 mg) and MeOH (0.4 mL) was stirred under normal pressure of hydrogen atmosphere at room temperature for 1 day. The mixture was concentrated under reduced pressure, and the residue was purified by silica gel column chromatography (ethyl acetate/hexane) to give the title compound (5.0 mg).

¹H NMR (300 MHz, CDCl₃) δ 1.31-1.64 (9H, m), 1.76-1.98 (2H, m), 2.06-2.19 (4H, m), 2.21-2.43 (2H, m), 2.85-3.16 (5H, m), 3.57-3.65 (1H, m), 3.67-3.88 (3H, m), 5.20-5.47 (3H, m), 5.95 (1H, d, J=7.0 Hz), 6.30 (1H, d, J=8.5 Hz), 7.07-7.17 (1H, m).

Example 111 rel-N-[(3S,4R)-4-({[(1s,4S)-4-cyclopropylcyclohexyl]oxy}methyl)-7-methyl-6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]methanesulfonamide with a Shorter Retention Time by HPLC (Column: CHIRALCEL OJ-H, Mobile Phase: Hexane/EtOH) A) [(1r,4r)-4-{[tert-butyl(dimethyl)silyl]oxy}cyclohexyl]methanol

To a mixture of ethyl trans-4-hydroxycyclohexanecarboxylate (18.8 g), 1H-imidazole (8.35 g) and DMF (150 mL) was added a mixture of tert-butyl(chloro)dimethylsilane (20 g) and DMF (80 mL) at room temperature, and the mixture was stirred overnight at room temperature. To the mixture was added water, and the mixture was extracted with diethyl ether. The organic layer was separated, washed with water and saturated brine, dried over anhydrous sodium sulfate, and concentrated under reduced pressure. The residue was diluted with THF (250 mL), the mixture was added to a mixture of 2.5 M aqueous lithium aluminium hydride THF solution (66 mL) and THF (130 mL) at 0° C. The mixture was stirred under nitrogen atmosphere at 0° C. for 1 hr. To the mixture was added successively water (6.6 mL), 4 M aqueous sodium hydroxide solution (6.6 mL) and water (17 mL) at 0° C. The mixture was stirred at room temperature for 15 min, and anhydrous magnesium sulfate was added thereto. The mixture was stirred at room temperature for 5 min, the insoluble substance was removed by filtration, and the filtrate was concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/hexane) to give the title compound (26.5 g).

¹H NMR (300 MHz, CDCl₃) δ 0.03-0.08 (6H, m), 0.82-1.10 (11H, m), 1.21-1.53 (4H, m), 1.74-1.95 (4H, m), 3.40-3.59 (3H, m).

B) (1r,4r)-4-{[tert-butyl(dimethyl)silyl]oxy}cyclohexane-1-carbaldehyde

To a mixture of sulfur trioxide pyridine complex (51.8 g) and DMSO (260 mL) was added a mixture of [(1r,4r)-4-{[tert-butyl(dimethyl)silyl]oxy}cyclohexyl]methanol (26.5 g), TEA (109 g) and EtOAc (100 ml) at 0° C. (internal temperature: 15° C. to 25° C.). The mixture was stirred under nitrogen atmosphere at room temperature for 30 min. To the mixture was added water at 0° C. (internal temperature: 15° C. to 30° C.), and the mixture was extracted with ethyl acetate. The organic layer was separated, washed successively with 0.1 M hydrochloric acid, water and saturated brine, dried over anhydrous magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/hexane) to give the title compound (21.8 g).

¹H NMR (300 MHz, CDCl₃) δ 0.05 (6H, s), 0.86-0.91 (9H, m), 1.29-1.46 (4H, m), 1.78-2.07 (4H, m), 2.11-2.28 (1H, m), 3.46-3.70 (1H, m), 9.64 (1H, d, J=1.1 Hz).

C) tert-butyl{[(1r,4r)-4-ethenylcyclohexyl]oxy}dimethylsilane

To a mixture of methyl(triphenyl)phosphonium bromide (79.2 g) and THF (450 mL) was added 1.6 M n-BuLi hexane solution (140 mL) at 0° C. The mixture was stirred under nitrogen atmosphere at 0° C. for 20 min, and to the mixture was added a mixture of (1r,4r)-4-{[tert-butyl(dimethyl)silyl]oxy}cyclohexane-1-carbaldehyde (26.9 g) and THF (50 mL) at 0° C. The mixture was stirred under nitrogen atmosphere at 0° C. for 1 hr. To the mixture was added saturated aqueous ammonium chloride solution at 0° C., and the mixture was extracted with a mixture of ethyl acetate-hexane (1:1). The organic layer was separated, washed with water and saturated brine, dried over anhydrous sodium sulfate, and concentrated under reduced pressure. The residue was diluted with a mixture of ethyl acetate-hexane (1:9), and the solid was triturated. The insoluble substance was removed by filtration from the mixture, and the filtrate was concentrated. The residue was purified by silica gel column chromatography (ethyl acetate/hexane) to give the title compound (18.7 g).

¹H NMR (400 MHz, CDCl₃) δ 0.05 (6H, s), 0.89 (9H, s), 1.01-1.51 (4H, m), 1.69-1.98 (5H, m), 3.46-3.58 (1H, m), 4.84-5.02 (2H, m), 5.68-5.81 (1H, m).

D) tert-butyl{[(1r,4r)-4-cyclopropylcyclohexyl]oxy}dimethylsilane

To dichloromethane (53 mL) was added a mixture of 1 M diethylzinc hexane solution (85 mL), TFA (9.48 g) and dichloromethane (60 mL) at 0° C. The mixture was stirred under nitrogen atmosphere at 0° C. for 10 min, and to the mixture was added a mixture of diiodomethane (22.3 g) and dichloromethane (30 mL) at 0° C. The mixture was stirred under nitrogen atmosphere at 0° C. for 10 min, and to the mixture was added a mixture of tert-butyl{[(1r,4r)-4-ethenylcyclohexyl]oxy}dimethylsilane (10.1 g) and dichloromethane (45 mL). The mixture was stirred under nitrogen atmosphere at room temperature for 1 hr. The mixture was diluted with saturated aqueous ammonium chloride solution, and concentrated under reduced pressure. To the residue was added saturated aqueous ammonium chloride solution, and the mixture was extracted with ethyl acetate. The organic layer was separated, washed with water and saturated brine, dried over anhydrous sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/hexane) to give the title compound (10.3 g).

¹H NMR (300 MHz, CDCl₃) δ 0.01-0.09 (8H, m), 0.31-0.50 (4H, m), 0.86-0.90 (9H, m), 1.00-1.33 (4H, m), 1.73-1.91 (4H, m), 3.45-3.60 (1H, m).

E) (1r,4r)-4-cyclopropylcyclohexyl methanesulfonate

To a mixture of tert-butyl{[(1r,4r)-4-cyclopropylcyclohexyl]oxy}dimethylsilane (10.3 g) and EtOH (100 mL) was added 6 M hydrochloric acid (10 mL). The mixture was stirred under nitrogen atmosphere at 40° C. for 30 min. To the mixture was added water at 0° C., and the mixture was extracted with diethyl ether. The organic layer was separated, washed with water and saturated brine, dried over anhydrous magnesium sulfate, and concentrated under reduced pressure. The residue was diluted with THF (100 mL), and methanesulfonic anhydride (10.2 g) and TEA (8.0 g) were successively added thereto at 0° C. The mixture was stirred under nitrogen atmosphere at room temperature for 1 hr, and concentrated under reduced pressure, and the residue was purified by silica gel column chromatography (ethyl acetate/hexane) to give the title compound (8.04 g).

¹H NMR (300 MHz, CDCl₃) δ 0.01-0.09 (2H, m), 0.35-0.55 (4H, m), 1.12-1.30 (2H, m), 1.41-1.59 (2H, m), 1.85-1.97 (2H, m), 2.10-2.22 (2H, m), 3.00 (3H, s), 4.54-4.69 (1H, m).

F) 6-({[(1s,4s)-4-cyclopropylcyclohexyl]oxy}methyl)-7-hydroxy-3-methyl-6,7,8,9-tetrahydro-4H-quinolizin-4-one

A mixture of 7-hydroxy-6-(hydroxymethyl)-3-methyl-6,7,8,9-tetrahydro-4H-quinolizin-4-one (3.10 g), (1r,4r)-4-cyclopropylcyclohexyl methanesulfonate (8.04 g), 2,8-dimethyl-10H-phenoxaborinin-10-ol (3.50 g), cesium carbonate (12.0 g) and acetonitrile (75 mL) was stirred at 80° C. (internal temperature) for 4 days. The mixture was concentrated under reduced pressure, water was added thereto, and the mixture was extracted with ethyl acetate. The organic layer was separated, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/hexane) to give the title compound (1.38 g).

MS: [M+H]⁺ 332.2.

G) 4-({[(1s,4s)-4-cyclopropylcyclohexyl]oxy}methyl)-7-methyl-2H-quinolizine-3,6(1H,4H)-dione

To a mixture of 6-({[(1s,4s)-4-cyclopropylcyclohexyl]oxy}methyl)-7-hydroxy-3-methyl-6,7,8,9-tetrahydro-4H-quinolizin-4-one (1.38 g) and acetonitrile (42 mL) was added Dess-Martin Periodinane (2.12 g) at room temperature, and the mixture was stirred for 1 hr. The mixture was concentrated under reduced pressure, and the residue was purified by silica gel column chromatography (ethyl acetate/hexane) to give the title compound (1.16 g).

MS: [M+H]⁺ 330.2.

H) rac-(6R,7S)-7-amino-6-({[(1s,4S)-4-cyclopropylcyclohexyl]oxy}methyl)-3-methyl-6,7,8,9-tetrahydro-4H-quinolizin-4-one

A mixture of 4-({[(1s,4s)-4-cyclopropylcyclohexyl]oxy}methyl)-7-methyl-2H-quinolizine-3,6(1H,4H)-dione (1.04 g), ammonium formate (1.99 g), AcOH (0.190 g), chloro[4-(dimethylamino)-2-pyridinecarboxamidato] (pentamethylcyclopentadienyl)iridium(III) (0.017 g) and MeOH (16 mL) was stirred at 60° C. for 1 hr. The mixture was concentrated under reduced pressure, and the residue was diluted with ethyl acetate. The mixture was washed with saturated aqueous sodium hydrogencarbonate solution and saturated brine, dried over anhydrous sodium sulfate, and concentrated under reduced pressure to give the title compound (1.01 g).

MS: [M+H]⁺ 331.3.

I) rac-N-[(3S,4R)-4-({[(1s,4S)-4-cyclopropylcyclohexyl]oxy}methyl)-7-methyl-6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]methanesulfonamide

To a mixture of rac-(6R,7S)-7-amino-6-({[(1s,4S)-4-cyclopropylcyclohexyl]oxy}methyl)-3-methyl-6,7,8,9-tetrahydro-4H-quinolizin-4-one (111 mg) and THF (1.7 mL) were added TEA (54 mg) and methanesulfonic anhydride (70.2 mg) at 0° C. The mixture was stirred for 15 min, to the mixture was added water, and the mixture was concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/hexane) to give the title compound (114 mg).

MS: [M+H]⁺ 409.2.

J) rel-N-[(3S,4R)-4-({[(1s,4S)-4-cyclopropylcyclohexyl]oxy}methyl)-7-methyl-6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]methanesulfonamide with a Shorter Retention Time by HPLC (Column: CHIRALCEL OJ-H, Mobile Phase: Hexane/EtOH)

rac-N-[(3S,4R)-4-({[(1s,4S)-4-Cyclopropylcyclohexyl]oxy}methyl)-7-methyl-6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]methanesulfonamide (114 mg) was resolved by HPLC (column: CHIRALCEL OJ, 50 mmID×500 mmL, mobile phase: hexane/ethanol=800/200) to give the title compound (41.9 mg).

¹H NMR (300 MHz, CDCl₃) δ −0.03-0.06 (2H, m), 0.32-0.42 (2H, m), 0.43-0.68 (2H, m), 1.25-1.46 (4H, m), 1.57-1.67 (2H, m), 1.76-2.00 (2H, m), 2.05-2.20 (4H, m), 2.21-2.37 (1H, m), 2.87-3.14 (5H, m), 3.55-3.63 (1H, m), 3.66-3.86 (3H, m), 5.25-5.38 (1H, m), 5.95 (1H, d, J=7.0 Hz), 6.36 (1H, d, J=8.5 Hz), 7.13 (1H, dd, J=6.9, 1.0 Hz).

Example 112 rel-N-[(3S,4R)-4-({[(1s,4S)-4-cyclopropylcyclohexyl]oxy}methyl)-7-methyl-6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]ethanesulfonamide with a Shorter Retention Time by HPLC (Column: CHIRALCEL OJ-H, Mobile Phase: Hexane/EtOH) A) rac-N-[(3S,4R)-4-({[(1s,4S)-4-cyclopropylcyclohexyl]oxy}methyl)-7-methyl-6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]ethanesulfonamide

A mixture of rac-(6R,7S)-7-amino-6-({[(1s,4S)-4-cyclopropylcyclohexyl]oxy}methyl)-3-methyl-6,7,8,9-tetrahydro-4H-quinolizin-4-one (111 mg) and THF (1.7 mL) was stirred, and TEA (102 mg) and ethanesulfonyl chloride (64.8 mg) were added thereto at 0° C. The mixture was stirred for 15 min, to the mixture was added water, and the mixture was concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/hexane) to give the title compound (100 mg).

MS: [M+H]⁺ 423.3.

B) rel-N-[(3S,4R)-4-({[(1s,4S)-4-cyclopropylcyclohexyl]oxy}methyl)-7-methyl-6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]ethanesulfonamide with a Shorter Retention Time by HPLC (Column: CHIRALCEL OJ-H, Mobile Phase: Hexane/EtOH)

rac-N-[(3S,4R)-4-({[(1s,4S)-4-Cyclopropylcyclohexyl]oxy}methyl)-7-methyl-6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]ethanesulfonamide (88 mg) was resolved by HPLC (column: CHIRALCEL OJ, 50 mmID×500 mmL, mobile phase: hexane/ethanol=850/150) to give the title compound (38.5 mg).

¹H NMR (300 MHz, CDCl₃) δ −0.03-0.07 (2H, m), 0.32-0.42 (2H, m), 0.44-0.66 (2H, m), 1.25-1.50 (7H, m), 1.51-1.67 (2H, m), 1.76-1.99 (2H, m), 2.03-2.20 (4H, m), 2.22-2.37 (1H, m), 2.86-3.14 (4H, m), 3.54-3.62 (1H, m), 3.64-3.82 (3H, m), 5.23-5.36 (1H, m), 5.94 (1H, d, J=7.0 Hz), 6.28 (1H, d, J=8.6 Hz), 7.12 (1H, dd, J=7.0, 0.9 Hz).

Example 113 rel-N-[(3S,4R)-4-({[(1s,4S)-4-cyclopropylcyclohexyl]oxy}methyl)-7-methyl-6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]cyclopropanesulfonamide with a Shorter Retention Time by HPLC (Column: CHIRALCEL OJ-H, Mobile Phase: Hexane/EtOH) A) rac-N-[(3S,4R)-4-({[(1s,4S)-4-cyclopropylcyclohexyl]oxy}methyl)-7-methyl-6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]cyclopropanesulfonamide

To a stirred mixture of rac-(6R,7S)-7-amino-6-({[(1s,4S)-4-cyclopropylcyclohexyl]oxy}methyl)-3-methyl-6,7,8,9-tetrahydro-4H-quinolizin-4-one (111 mg) and THF (1.7 mL) were added TEA (204 mg), cyclopropanesulfonyl chloride (142 mg) and DMAP (41.0 mg) at room temperature. The mixture was stirred at 60° C. for 1 hr. To the mixture was added water, and the mixture was concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/hexane) to give the title compound (109 mg).

MS: [M+H]⁺ 435.2.

B) rel-N-[(3S,4R)-4-({[(1s,4S)-4-cyclopropylcyclohexyl]oxy}methyl)-7-methyl-6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]cyclopropanesulfonamide with a Shorter Retention Time by HPLC (Column: CHIRALCEL OJ-H, Mobile Phase: Hexane/EtOH)

rac-N-[(3S,4R)-4-({[(1s,4S)-4-Cyclopropylcyclohexyl]oxy}methyl)-7-methyl-6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]cyclopropanesulfonamide (96.7 mg) was resolved by HPLC (column: CHIRALCEL OJ, 50 mmID×500 mmL, mobile phase: hexane/ethanol=850/150) to give the title compound (43.2 mg).

¹H NMR (300 MHz, CDCl₃) δ −0.03-0.07 (2H, m), 0.33-0.44 (2H, m), 0.45-0.66 (2H, m), 0.98-1.50 (8H, m), 1.51-1.70 (2H, m), 1.75-1.98 (2H, m), 2.02-2.19 (4H, m), 2.21-2.36 (1H, m), 2.43-2.56 (1H, m), 2.86-3.15 (2H, m), 3.51-3.64 (1H, m), 3.65-3.89 (3H, m), 5.39-5.48 (1H, m), 5.95 (1H, d, J=7.0 Hz), 6.31 (1H, d, J=8.6 Hz), 7.13 (1H, dd, J=7.0, 1.0 Hz).

Example 121 rac-N-[(3S,4R)-7-methyl-6-oxo-4-({[(1s,4S)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]ethanesulfonamide

To a mixture of rac-(6R,7S)-7-amino-3-methyl-6-({[(1s,4S)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-6,7,8,9-tetrahydro-4H-quinolizin-4-one (1.40 g) and dichloromethane (25 mL) were added TEA (1.29 g) and ethanesulfonyl chloride (1.10 g) at 0° C. The mixture was stirred under nitrogen atmosphere at 25° C. for 14 hr. To the mixture was added water, and the mixture was extracted with dichloromethane. The organic layer was separated, washed with saturated brine, dried over anhydrous sodium sulfate, and concentrated under reduced pressure. The residue was purified by HPLC (C18, mobile phase: water/acetonitrile (containing 0.05% NH₃·H₂O)), and freeze-dried to give the title compound (760 mg).

MS: [M+H]⁺ 421.3.

Example 122 rac-N-[(3S,4R)-7-methyl-6-oxo-4-({[(1s,4S)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]cyclopropanesulfonamide

To a mixture of rac-(6R,7S)-7-amino-3-methyl-6-({[(1s,4S)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-6,7,8,9-tetrahydro-4H-quinolizin-4-one (500 mg) and dichloromethane (10 mL) were added TEA (462 mg) and cyclopropanesulfonyl chloride (321 mg) at 0° C. The mixture was stirred under nitrogen atmosphere at 20° C. for 14 hr. To the mixture was added water, and the mixture was extracted with ethyl acetate. The organic layer was separated, washed with saturated brine, dried over anhydrous sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/petroleum ether) to give the title compound (350 mg).

MS: [M+H]⁺ 433.4.

Example 144 rel-N,N-dimethyl-N′-[(3S,4R)-7-methyl-6-oxo-4-({[(1s,4S)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]sulfuric diamide with a Shorter Retention Time by SFC (Column: CHIRALPAK AS-3, Mobile Phase: Carbon Dioxide/0.05% Diethylamine in EtOH) A) rac-N,N-dimethyl-N′-[(3S,4R)-7-methyl-6-oxo-4-({[(1s,4S)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]sulfuric diamide

To a mixture of rac-(6R,7S)-7-amino-3-methyl-6-({[(1s,4S)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-6,7,8,9-tetrahydro-4H-quinolizin-4-one (200 mg) and dichloromethane (10 mL) were added TEA (246 mg) and dimethylsulfamoyl chloride (262 mg) at 25° C., and the mixture was stirred under nitrogen atmosphere at room temperature for 14 hr. To the mixture was added water, and the mixture was extracted with ethyl acetate. The organic layer was washed with saturated brine, dried over anhydrous sodium sulfate, and concentrated under reduced pressure. The residue was purified by HPLC (C18, mobile phase: water/acetonitrile (containing 0.05% NH₃·H₂O)), and the obtained fraction was concentrated. The residue was freeze-dried to give the title compound (20.1 mg).

¹H NMR (400 MHz, CDCl₃) δ 1.44-1.55 (2H, m), 1.58-1.74 (5H, m), 1.76-1.86 (4H, m), 2.00-2.15 (4H, m), 2.23-2.36 (1H, m), 2.39-2.51 (1H, m), 2.83 (6H, s), 2.87-3.10 (2H, m), 3.36-3.47 (1H, m), 3.63-3.75 (2H, m), 3.76-3.84 (1H, m), 5.23-5.33 (1H, m), 5.93 (1H, d, J=6.8 Hz), 5.99 (1H, d, J=8.8 Hz), 7.11 (1H, d, J=7.2 Hz).

B) rel-N,N-dimethyl-N′-[(3S,4R)-7-methyl-6-oxo-4-({[(1s,4S)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]sulfuric diamide with a Shorter Retention Time by SFC (Column: CHIRALPAK AS-3, Mobile Phase: Carbon Dioxide/0.05% Diethylamine in EtOH)

rac-N,N-Dimethyl-N′-[(3S,4R)-7-methyl-6-oxo-4-({[(1s,4S)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]sulfuric diamide (15.0 mg) was resolved by SFC (column: CHIRALPAK AS, 30 mmID×250 mmL, mobile phase: carbon dioxide/EtOH (containing 0.1% NH₃·H₂O)=850/150) and HPLC (C18, mobile phase: water/acetonitrile (containing 0.05% NH₃·H₂O)). The obtained fraction was concentrated, and the residue was freeze-dried to give the title compound (2.2 mg).

¹H NMR (400 MHz, CDCl₃) δ 1.44-1.56 (2H, m), 1.59-1.74 (5H, m), 1.75-1.86 (4H, m), 2.00-2.15 (4H, m), 2.23-2.36 (1H, m), 2.39-2.50 (1H, m), 2.82 (6H, s), 2.87-3.10 (2H, m), 3.36-3.47 (1H, m), 3.63-3.75 (2H, m), 3.76-3.84 (1H, m), 5.23-5.33 (1H, m), 5.93 (1H, d, J=6.8 Hz), 5.98 (1H, d, J=8.4 Hz), 7.12 (1H, d, J=7.2 Hz).

Example 145 rel-N-[(3S,4R)-4-({[(1s,4S)-4-ethoxycyclohexyl]oxy}methyl)-7-methyl-6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]methanesulfonamide with a Longer Retention Time by SFC (Column: CHIRALPAK AD-3, Mobile Phase: Carbon Dioxide/0.05% Diethylamine in EtOH) A) (1r,4r)-4-{[tert-butyl(dimethyl)silyl]oxy}cyclohexan-1-ol

To a mixture of trans-cyclohexane-1,4-diol (20.0 g) and DMF (200 mL) were added 1H-imidazole (17.6 g) and tert-butyl(chloro)dimethylsilane (28.6 g) at 0° C. The mixture was stirred at 0° C. for 1 hr. To the mixture was added water, and the mixture was extracted with ethyl acetate. The organic layer was separated, dried over anhydrous sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/petroleum ether) to give the title compound (32.1 g).

¹H NMR (400 MHz, CDCl₃) δ 0.05 (6H, s), 0.88 (9H, s), 1.22-1.48 (4H, m), 1.76-2.01 (4H, m), 3.52-3.83 (2H, m).

B) tert-butyl{[(1r,4r)-4-ethoxycyclohexyl]oxy}dimethylsilane

To a mixture of (1r,4r)-4-{[tert-butyl(dimethyl)silyl]oxy}cyclohexan-1-ol (9 g) and DMF (50 mL) was added 60% sodium hydride (7.81 g) at 0° C. The reaction mixture was stirred at the same temperature for 10 min, and iodoethane (15.6 mL) was added thereto at 0° C. The mixture was stirred at room temperature for 16 hr. The mixture was poured into water, and extracted with ethyl acetate. The organic layer was separated, washed with water and saturated brine, dried over anhydrous magnesium sulfate, and concentrated under reduced pressure to give the title compound (8.15 g).

¹H NMR (300 MHz, CDCl₃) δ 0.00 (6H, s), 0.83 (9H, s), 1.08-1.18 (3H, m), 1.21-1.36 (4H, m), 1.72-1.85 (2H, m), 1.85-1.98 (2H, m), 3.12-3.29 (1H, m), 3.37-3.48 (2H, m), 3.51-3.67 (1H, m).

C) (1r,4r)-4-ethoxycyclohexan-1-ol

To a mixture of tert-butyl{[(1r,4r)-4-ethoxycyclohexyl]oxy}dimethylsilane (7.25 g) and THF (1 mL) was added 1M tetrabutylammonium fluoride THF solution (56 mL) at room temperature, and the mixture was stirred at room temperature for 12 hr. The mixture was concentrated under reduced pressure, and the residue was purified by silica gel column chromatography (ethyl acetate/hexane) to give the title compound (2.43 g).

¹H NMR (300 MHz, CDCl₃) δ 1.15-1.23 (3H, m), 1.28-1.37 (4H, m), 1.42-1.46 (1H, m), 1.89-2.09 (4H, m), 3.19-3.31 (1H, m), 3.49 (2H, s), 3.60-3.75 (1H, m).

D) (1r,4r)-4-ethoxycyclohexyl methanesulfonate

To a mixture of (1r,4r)-4-ethoxycyclohexan-1-ol (20.1 g) and dichloromethane (150 mL) was added TEA (42.3 g), and methanesulfonyl chloride (31.9 g) was added thereto at 0° C. little by little. The mixture was stirred at 20° C. for 2 hr. The mixture was diluted with water, and extracted with dichloromethane. The organic layer was separated, washed with saturated brine, dried over anhydrous sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/petroleum ether) to give the title compound (20.2 g).

¹H NMR (400 MHz, CDCl₃) δ 1.17 (3H, t, J=6.8 Hz), 1.42-1.53 (2H, m), 1.60-1.68 (2H, m), 1.91-2.01 (2H, m), 2.04-2.14 (2H, m), 2.99 (3H, s), 3.30-3.40 (1H, m), 3.47 (2H, q, J=6.4 Hz), 4.65-4.80 (1H, m).

E) 6-{[(4-ethoxycyclohexyl)oxy]methyl}-7-hydroxy-3-methyl-6,7,8,9-tetrahydro-4H-quinolizin-4-one

To a mixture of 7-hydroxy-6-(hydroxymethyl)-3-methyl-6,7,8,9-tetrahydro-4H-quinolizin-4-one (2.50 g) and acetonitrile (30 mL) were added (1r,4r)-4-ethoxycyclohexyl methanesulfonate (7.97 g), cesium carbonate (19.5 g) and 2,8-dimethyl-10H-phenoxaborinin-10-ol (3.21 g). The mixture was stirred at 120° C. for 48 hr in a sealed tube. The mixture was diluted with water, and extracted with ethyl acetate. The organic layer was separated, washed with saturated brine, dried over anhydrous sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/petroleum ether) to give the title compound (2.65 g).

MS: [M+H]⁺ 336.2.

F) 4-{[(4-ethoxycyclohexyl)oxy]methyl}-7-methyl-2H-quinolizine-3,6(1H,4H)-dione

To a mixture of 6-{[(4-ethoxycyclohexyl)oxy]methyl}-7-hydroxy-3-methyl-6,7,8,9-tetrahydro-4H-quinolizin-4-one (5.30 g) and dichloromethane (50 mL) was added Dess-Martin Periodinane (13.4 g). The mixture was stirred at 25° C. for 2 hr. To the mixture was added saturated aqueous sodium hydrogencarbonate solution, and the mixture was extracted with dichloromethane. The organic layer was separated, washed with saturated brine, dried over anhydrous sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/petroleum ether) to give the title compound (4.92 g).

MS: [M+H]⁺ 334.0.

G) (6RS,7SR)-7-amino-6-{[(4-ethoxycyclohexyl)oxy]methyl}-3-methyl-6,7,8,9-tetrahydro-4H-quinolizin-4-one

To a mixture of 4-{[(4-ethoxycyclohexyl)oxy]methyl}-7-methyl-2H-quinolizine-3,6(1H,4H)-dione (4.92 g) and EtOH (50 mL) were added hydroxylamine hydrochloride (2.05 g) and sodium acetate (2.42 g). The mixture was stirred at 80° C. for 30 min, and concentrated under reduced pressure. The residue was diluted with water, and the mixture was extracted with ethyl acetate. The organic layer was separated, washed with saturated brine, dried over anhydrous sodium sulfate, and concentrated under reduced pressure. To a mixture of the residue and MeOH (50 mL) were added molybdenum(VI) oxide (3.30 g) and sodium borohydride (2.89 g) at 0° C. little by little. The mixture was stirred under nitrogen atmosphere at 25° C. for 1 hr. To the mixture was added water, and the mixture was extracted with ethyl acetate. The organic layer was separated, washed with saturated brine, dried over anhydrous sodium sulfate, and concentrated under reduced pressure to give the title compound (3.84 g).

MS: [M+H]⁺ 335.0.

H) N-[(3SR,4RS)-4-({[4-ethoxycyclohexyl]oxy}methyl)-7-methyl-6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]methanesulfonamide

To a stirred mixture of (6RS,7SR)-7-amino-6-{[(4-25 ethoxycyclohexyl)oxy]methyl}-3-methyl-6,7,8,9-tetrahydro-4H-quinolizin-4-one (1.84 g), TEA (2.23 g) and dichloromethane (20 mL) was added methanesulfonyl chloride at 0° C. little by little. The mixture was stirred under nitrogen atmosphere at 25° C. for 1 hr. To the mixture was added water, and the mixture was extracted with dichloromethane. The organic layer was separated, dried over anhydrous sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/petroleum ether) to give the title compound (1.70 g).

MS: [M+H]⁺ 413.4.

I) rel-N-[(3S,4R)-4-({[(1s,4S)-4-ethoxycyclohexyl]oxy}methyl)-7-methyl-6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]methanesulfonamide with a Longer Retention Time by SFC (Column: CHIRALPAK AD-3, Mobile Phase: Carbon Dioxide/0.05% Diethylamine in EtOH)

Each batch of N-[(3SR,4RS)-4-({[4-ethoxycyclohexyl]oxy}methyl)-7-methyl-6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]methanesulfonamide (900 mg and 700 mg) was resolved by SFC (column: CHIRALPAK AD, 30 mmID×250 mmL, mobile phase: carbon dioxide/EtOH (containing 0.1% NH₃·H₂O)=750/250) Separately, as another batch, N-[(3SR,4RS)-4-({[4-ethoxycyclohexyl]oxy}methyl)-7-methyl-6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]methanesulfonamide (1.70 g) was resolved by SFC (column: CHIRALPAK AD, 30 mmID×250 mmL, mobile phase: carbon dioxide/EtOH (containing 0.1% NH₃·H₂O)=750/250). In each batch, the fractions having the peak with a longest retention time among the major four peaks were collected. These fractions were combined, and concentrated under reduced pressure. The residue was purified by HPLC (C18, mobile phase: water/acetonitrile (containing 0.05% NH₃·H₂O)), and the obtained fraction was concentrated. The residue was freeze-dried to give the title compound (255 mg).

¹H NMR (400 MHz, CDCl₃) δ 1.18 (3H, t, J=7.2 Hz), 1.58-1.66 (5H, m), 1.68-1.76 (2H, m), 1.78-1.91 (1H, m), 2.04-2.16 (4H, m), 2.18-2.32 (1H, m), 2.88-3.10 (5H, m), 3.28-3.37 (1H, m), 3.41-3.52 (3H, m), 3.66-3.87 (3H, m), 5.23-5.31 (1H, m), 5.94 (1H, d, J=7.2 Hz), 6.12 (1H, d, J=8.8 Hz), 7.12 (1H, d, J=7.2 Hz).

Example 146 rel-N-[(3S,4R)-7-methyl-6-oxo-4-({[(1s,4S)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]methanesulfonamide with a Longer Retention Time by SFC (Column: CHIRALPAK AD-3, Mobile Phase: Carbon Dioxide/0.05% Diethylamine in EtOH)

rac-N-[(3S,4R)-7-Methyl-6-oxo-4-({[(1s,4S)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]methanesulfonamide (550 mg) was resolved by SFC (column: CHIRALPAK AD, 30 mmID×250 mmL, mobile phase: carbon dioxide/EtOH (containing 0.1% NH₃·H₂O)=750/250). The obtained fraction was concentrated under reduced pressure, and freeze-dried to give the title compound (193 mg).

¹H NMR (400 MHz, CDCl₃) δ 1.46-1.58 (2H, m), 1.60-1.76 (5H, m), 1.77-1.88 (4H, m), 2.03-2.16 (4H, m), 2.21-2.33 (1H, m), 2.40-2.52 (1H, m), 2.87-3.11 (5H, m), 3.37-3.48 (1H, m), 3.67-3.85 (3H, m), 5.22-5.32 (1H, m), 5.95 (1H, d, J=6.8 Hz), 6.20 (1H, d, J=8.8 Hz), 7.13 (1H, d, J=6.8 Hz).

Example 147 rel-N-[(3S,4R)-7-methyl-6-oxo-4-({[(1s,4S)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]ethanesulfonamide with a Shorter Retention Time by SFC (Column: CHIRALCEL OJ-3, Mobile Phase: Carbon Dioxide/0.05% Diethylamine in EtOH)

rac-N-[(3S,4R)-7-Methyl-6-oxo-4-({[(1s,4S)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]ethanesulfonamide (760 mg) was resolved by SFC (column: CHIRALCEL OJ-H, 30 mmID×250 mmL, mobile phase: carbon dioxide/EtOH (containing 0.1% NH₃·H₂O)=800/200). The obtained fraction was concentrated, and the residue was freeze-dried to give the title compound (293 mg).

¹H NMR (400 MHz, CDCl₃) δ 1.43 (3H, t, J=7.2 Hz), 1.48-1.58 (2H, m), 1.60-1.75 (5H, m), 1.76-1.87 (4H, m), 2.03-2.17 (4H, m), 2.22-2.34 (1H, m), 2.40-2.51 (1H, m), 2.88-3.14 (4H, m), 3.36-3.48 (1H, m), 3.66-3.84 (3H, m), 5.20-5.31 (1H, m), 5.94 (1H, d, J=6.8 Hz), 6.11 (1H, d, J=8.4 Hz), 7.12 (1H, d, J=6.8 Hz).

Example 148 rel-N-[(3S,4R)-7-methyl-6-oxo-4-({[(1s,4S)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]cyclopropanesulfonamide with a Longer Retention Time by SFC (Column: CHIRALPAK AD-3, Mobile Phase: Carbon Dioxide/0.05% Diethylamine in EtOH)

rac-N-[(3S,4R)-7-Methyl-6-oxo-4-({[(1s,4S)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]cyclopropanesulfonamide (770 mg) was resolved by SFC (column: CHIRALPAK AD, 30 mmID×250 mmL, mobile phase: carbon dioxide/EtOH (containing 0.1% NH₃·H₂O)=650/350) and HPLC (C18, mobile phase: water/acetonitrile (containing 0.05% NH₃·H₂O)). The obtained fraction was concentrated, and the residue was freeze-dried to give the title compound (173 mg).

¹H NMR (400 MHz, CDCl₃) δ 0.98-1.28 (4H, m), 1.47-1.58 (2H, m), 1.62-1.75 (5H, m), 1.76-1.86 (4H, m), 2.02-2.17 (4H, m), 2.21-2.32 (1H, m), 2.40-2.56 (2H, m), 2.87-3.11 (2H, m), 3.37-3.47 (1H, m), 3.67-3.87 (3H, m), 5.34-5.44 (1H, m), 5.94 (1H, d, J=6.8 Hz), 6.15 (1H, d, J=8.8 Hz), 7.12 (1H, d, J=7.2 Hz).

Example 149 rel-N-methyl-N′-[(3S,4R)-7-methyl-6-oxo-4-({[(1s,4S)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]sulfuric diamide with a Longer Retention Time by HPLC (Column: CHIRALPAK IC, Mobile Phase: Hexane/EtOH) A) rac-N-methyl-N′-[(3S,4R)-7-methyl-6-oxo-4-({[(1s,4S)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]sulfuric diamide

To a mixture of rac-(6R,7S)-7-amino-3-methyl-6-({[(1s,4S)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-6,7,8,9-tetrahydro-4H-quinolizin-4-one (102 mg), TEA (291 mg), N,N-dimethylpyridin-4-amine (11.3 mg) and THF (2.0 mL) was added a mixture of methylsulfamoyl chloride (150 mg) and THF (2.0 mL) at 0° C. The mixture was stirred at room temperature for 1 hr. To the mixture was added water, and the mixture was extracted with ethyl acetate. The organic layer was separated, washed with saturated brine, dried over anhydrous sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/hexane) to give the title compound (47.1 mg).

MS: [M+H]⁺ 422.1.

B) rel-N-methyl-N′-[(3S,4R)-7-methyl-6-oxo-4-({[(1s,4S)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]sulfuric diamide with a Longer Retention Time by HPLC (Column: CHIRALPAK IC, Mobile Phase: Hexane/EtOH)

rac-N-Methyl-N′-[(3S,4R)-7-methyl-6-oxo-4-({[(1s,4S)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]sulfuric diamide (40.0 mg) was resolved by HPLC (column: CHIRALPAK IC, 20 mmID×250 mmL, mobile phase: hexane/EtOH=600/400) to give the title compound (14.7 mg).

¹H NMR (300 MHz, CDCl₃) δ 1.47-1.88 (12H, m), 2.06-2.17 (3H, m), 2.22-2.36 (1H, m), 2.39-2.53 (1H, m), 2.80 (3H, d, J=5.3 Hz), 2.84-3.13 (2H, m), 3.35-3.47 (1H, m), 3.61-3.88 (3H, m), 4.16-4.34 (1H, m), 5.25-5.36 (1H, m), 5.89-6.13 (2H, m), 7.08-7.16 (1H, m).

The compounds of Examples are shown in the following 25 tables. MS in the tables means actual measured value. The compounds of Examples 2 to 5, 7, 8, 10 to 77, 79 to 108, 114 to 120 and 123 to 143 in the following tables were produced according to the methods described in the above-mentioned Examples, or methods analogous thereto.

TABLE 1-1 Ex. No. IUPAC Name Structure MS 1 rel-N-[(3S,4R)-7-methyl-4-({[(1s,4S)-4- methylcyclohexyl]oxy}methyl)-6-oxo-1,3,4,6- tetrahydro-2H-quinolizin-3-yl]methanesulfonamide with a longer retention time by SFC (column: CHIRALPAK AD-H, mobile phase: carbon dioxide/MeOH)

383.1 2 rac-N-[(3S,4R)-7-ethyl-4-({[(1s,4S)-4- methylcyclohexyl]oxy}methyl)-6-oxo-1,3,4,6- tetrahydro-2H-quinolizin-3-yl]methanesulfonamide

397.3 3 rac-N-[(3S,4R)-4-({[(1s,4S)-4- methylcyclohexyl]oxy}methyl)-6-oxo-1,3,4,6- tetrahydro-2H-quinolizin-3-yl]methanesulfonamide

369.2 4 N-[(3SR,4RS)-4-{[(4- methoxycyclohexyl)oxy]methyl}-7-methyl-6-oxo- 1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide

399.2 5 rel-N-[(3S,4R)-7-methyl-4-({[(1r,4R)-4- methylcyclohexyl]oxy}methyl)-6-oxo-1,3,4,6- tetrahydro-2H-quinolizin-3-yl]methanesulfonamide with a shorter retention time by HPLC (column: CHIRALCEL OD-H, mobile phase: hexane/EtOH)

383.2 6 rel-N-[(3S,4R)-7-methyl-6-oxo-4-({[(1s,4S)-4- phenylcyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro- 2H-quinolizin-3-yl]methanesulfonamide with a shorter retention time by SFC (column: CHIRALCEL OJ-H, mobile phase: carbon dioxide/MeOH)

445.1 7 rac-N-[(3S,4R)-7-methyl-4-({[(1s,4S)-4- methylcyclohexyl]oxy}methyl)-6-oxo-1,3,4,6- tetrahydro-2H-quinolizin-3-yl]ethanesulfonamide

397.1

TABLE 1-2 Ex. No. IUPAC Name Structure MS  8 rac-N-[(3S,4R)-7-methyl-4-({[(1s,4S)-4- methylcyclohexyl]oxy}methyl)-6-oxo-1,3,4,6- tetrahydro-2H-quinolizin-3- yl]cyclopropanesulfonamide

409.1  9 rel-N-[(3S,4R)-4-({[(1s,4S)-4- ethylcyclohexyl]oxy}methyl)-7-methyl-6-oxo- 1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide with a shorter retention time by SFC (column: CHIRALCEL OJ-H, mobile phase: carbon dioxide/MeOH)

397.1 10 rac-N-[(3S,4R)-4-({[(1s,4S)-4- ethylcyclohexyl]oxy}methyl)-7-methyl -6-oxo- 1,3,4,6-tetrahydro-2H-quinolizin-3- yl]cyclopropanesulfonamide

423.3 11 rac-N-[(3S,4R)-4-({[(1s,4S)-4- ethylcyclohexyl]oxy}methyl)-7-methyl-6-oxo- 1,3,4,6-tetrahydro-2H-quinolizin-3- yl]ethanesulfonamide

411.3 12 rac-N-[(3S,4R)-7-methyl-6-oxo-4-({[(1s,4S)-4- (propan-2-yl)cyclohexyl]oxy}methyl)-1,3,4,6- tetrahydro-2H-quinolizin-3-yl] methanesulfonamide

411.2 13 rac-N-[(3S,4R)-7-methyl-6-oxo-4-({[(1s,4S)-4- (propan-2-yl)cyclohexyl]oxy}methyl)-1,3,4,6- tetrahydro-2H-quinolizin-3-yl]ethanesulfonamide

425.3 14 rac-N-[(3S,4R)-7-methyl-6-oxo-4-({[(1s,4S)-4- (propan-2-yl)cyclohexyl]oxy}methyl)-1,3,4,6- tetrahydro-2H-quinolizin-3- yl]cyclopropanesulfonamide

437.2

TABLE 1-3 Ex. No. IUPAC Name Structure MS 15 rac-N-[(3S,4R)-7-methyl-6-oxo-4-({[(1s,4S)-4- phenylcyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro- 2H-quinolizin-3-yl]ethanesulfonamide

459.2 16 rac-N-[(3S,4R)-7-methyl-6-oxo-4-({[(1s,4S)-4- phenylcyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro- 2H-quinolizin-3-yl]cyclopropanesulfonamide

471.2 17 rac-N-[(3S,4R)-4-({[(1s,4S)-4-tert- butylcyclohexyl]oxy}methyl)-7-methyl-6-oxo- 1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide

425.3 18 rac-N-[(3S,4R)-4-({[(1s,4S)-4-tert- butylcyclohexyl]oxy}methyl)-7-methyl-6-oxo- 1,3,4,6-tetrahydro-2H-quinolizin-3- yl]ethanesulfonamide

439.3 19 rac-N-[(3S,4R)-7-methyl-4-({[(1s,4S)-4-(2- methylphenyl)cyclohexyl]oxy}methyl)-6-oxo-1,3,4,6- tetrahydro-2H-quinolizin-3-yl]methanesulfonamide

459.0 20 rac-N-[(3S,4R)-7-methyl-4-({[(1s,4S)-4-(2- methylphenyl)cyclohexyl]oxy}methyl)-6-oxo-1,3,4,6- tetrahydro-2H-quinolizin-3- yl]cyclopropanesulfonamide

485.0 21 rac-N-[(3S,4R)-7-methyl-4-({[(1s,4S)-4-(3- methylphenyl)cyclohexyl]oxy}methyl)-6-oxo-1,3,4,6- tetrahydro-2H-quinolizin-3-yl]methanesulfonamide

459.1

TABLE 1-4 Ex. No. IUPAC Name Structure MS 22 rac-N-[(3S,4R)-7-methyl-4-({[(1s,4S)-4-(3- methylphenyl)cyclohexyl]oxy}methyl)-6-oxo-1,3,4,6- tetrahydro-2H-quinolizin-3-yl]ethanesulfonamide

473.1 23 rac-N-[(3S,4R)-7-methyl-4-({[(1s,4S)-4-(2- methylphenyl)cyclohexyl]oxy}methyl)-6-oxo-1,3,4,6- tetrahydro-2H-quinolizin-3-yl]ethanesulfonamide

473.0 24 rac-N-[(3S,4R)-4-({[(1s,4S)-4-tert- butylcyclohexyl]oxy}methyl)-7-methyl-6-oxo- 1,3,4,6-tetrahydro-2H-quinolizin-3- yl]cyclopropanesulfonamide

451.1 25 rac-N-[(3S,4R)-7-methyl-4-({[(1s,4S)-4-(3- methylphenyl)cyclohexyl]oxy}methyl)-6-oxo-1,3,4,6- tetrahydro-2H-quinolizin-3- yl]cyclopropanesulfonamide

485.0 26 rac-N,N-dimethyl-N′-[(3S,4R)-7-methyl-4- ({[(1s,4S)-4-methylcyclohexyl]oxy}methyl)-6-oxo- 1,3,4,6-tetrahydro-2H-quinolizin-3-yl]sulfuric diamide

412.1 27 rac-N′-[(3S,4R)-4-({[(1s,4S)-4- ethylcyclohexyl]oxy}methyl)-7-methyl-6-oxo- 1,3,4,6-tetrahydro-2H-quinolizin-3-yl]-N,N- dimethylsulfuric diamide

426.3 28 rac-N,N-dimethyl-N′-[(3S,4R)-7-methyl-6-oxo-4- ({[(1s,4S)-4-(propan-2-yl)cyclohexyl]oxy}methyl)- 1,3,4,6-tetrahydro-2H-quinolizin-3-yl]sulfuric diamide

440.2

TABLE 1-5 Ex. No. IUPAC Name Structure MS 29 rac-N,N-dimethyl-N′-[(3S,4R)-7-methyl-6-oxo-4- ({[(1s,4S)-4-phenylcyclohexyl]oxy}methyl)-1,3,4,6- tetrahydro-2H-quinolizin-3-yl]sulfuric diamide

474.2 30 rac-N′-[(3S,4R)-4-({[(1s,4S)-4-tert- butylcyclohexyl]oxy}methyl)-7-methyl-6-oxo- 1,3,4,6-tetrahydro-2H-quinolizin-3-yl]-N,N- dimethylsulfuric diamide

454.3 31 rac-N,N-dimethyl-N′-[(3S,4R)-7-methyl-4- ({[(1s,4S)-4-(2- methylphenyl)cyclohexyl]oxy}methyl)-6-oxo-1,3,4,6- tetrahydro-2H-quinolizin-3-yl]sulfuric diamide

488.0 32 rac-N,N-dimethyl-N′-[(3S,4R)-7-methyl-4- ({[(1s,4S)-4-(3- methylphenyl)cyclohexyl]oxy}methyl)-6-oxo-1,3,4,6- tetrahydro-2H-quinolizin-3-yl]sulfuric diamide

488.3 33 rac-N-[(3S,4R)-7-methyl-4-({[(1s,4S)-4-(4- methylphenyl)cyclohexyl]oxy}methyl)-6-oxo-1,3,4,6- tetrahydro-2H-quinolizin-3-yl]methanesulfonamide

459.2 34 rac-N-[(3S,4R)-7-methyl-4-({[(1s,4S)-4-(4- methylphenyl)cyclohexyl]oxy}methyl)-6-oxo-1,3,4,6- tetrahydro-2H-quinolizin-3-yl]ethanesulfonamide

473.2 35 rac-N,N-dimethyl-N′-[(3S,4R)-7-methyl-4- ({[(1s,4S)-4-(4- methylphenyl)cyclohexyl]oxy}methyl)-6-oxo-1,3,4,6- tetrahydro-2H-quinolizin-3-yl]sulfuric diamide

488.3

TABLE 1-6 Ex. No. IUPAC Name Structure MS 36 rac-N-[(3S,4R)-7-methyl-4-({[(1s,4S)-4-(4- methylphenyl)cyclohexyl]oxy}methyl)-6-oxo-1,3,4,6- tetrahydro-2H-quinolizin-3- yl]cyclopropanesulfonamide

485.2 37 rac-N-[(3S,4R)-7-methyl-6-oxo-4-({[(1s,4S)-4- (trifluoromethyl)cyclohexyl]oxy}methyl)-1,3,4,6- tetrahydro-2H-quinolizin-3-yl]methanesulfonamide

437.2 38 rac-N-[(3S,4R)-7-methyl-6-oxo-4-({[(1s,4S)-4- (trifluoromethyl)cyclohexyl]oxy}methyl)-1,3,4,6- tetrahydro-2H-quinolizin-3-yl]ethanesulfonamide

451.3 39 rac-N-[(3S,4R)-4-({[(1s,4S)-4- (methoxymethyl)cyclohexyl]oxy}methyl)-7-methyl- 6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide

413.3 40 rac-N-[(3S,4R)-4-({[(1s,4S)-4-(4- fluorophenyl)cyclohexyl]oxy}methyl)-7-methyl-6- oxo-1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide

463.3 41 rac-N-[(3S,4R)-4-({[(1s,4S)-4-(4- fluorophenyl)cyclohexyl]oxy}methyl)-7-methyl-6- oxo-1,3,4,6-tetrahydro-2H-quinolizin-3- yl]ethanesulfonamide

477.3 42 rac-N-[(3S,4R)-4-({[(1s,4S)-4-(2- fluorophenyl)cyclohexyl]oxy}methyl)-7-methyl-6- oxo-1,3,4,6-tetrahydro-2H-quinolizin-3- yl]ethanesulfonamide

477.3

TABLE 1-7 Ex. No. IUPAC Name Structure MS 43 rac-N-[(3S,4R)-4-({[(1s,4S)-4-(2- fluorophenyl)cyclohexyl]oxy}methyl)- 7-methyl-6-oxo-1,3,4,6-tetrahydro-2H- quinolizin-3-yl]methanesulfonamide

463.3 44 rac-N-[(3S,4R)-4-({[(1s,4S)-4-(4- fluorophenyl)cyclohexyl]oxy}methyl)- 7-methyl-6-oxo-1,3,4,6-tetrahydro-2H- quinolizin-3-yl]cyclopropanesulfonamide

489.2 45 rac-N-[(3S,4R)-4-({[(1s,4S)-4-(2- fluorophenyl)cyclohexyl]oxy}methyl)- 7-methyl-6-oxo-1,3,4,6-tetrahydro-2H- quinolizin-3-yl]cyclopropanesulfonamide

489.3 46 rac-N′-[(3S,4R)-4-({[(1s,4S)-4-(4- fluorophenyl)cyclohexyl]oxy}methyl)- 7-methyl-6-oxo-1,3,4,6-tetrahydro-2H- quinolizin-3-yl]-N,N- dimethylsulfuric diamide

492.3 47 rac-N′-[(3S,4R)-4-({[(1s,4S)-4-(2- fluorophenyl)cyclohexyl]oxy}methyl)- 7-methyl-6-oxo-1,3,4,6-tetrahyd ro-2H- quinolizin-3-yl]-N,N- dimethylsulfuric diamide

492.2 48 rac-N-[(3S,4R)-4-({[(1s,4S)-4- ethylcyclohexyl]oxy}methyl)-7,9- dimethyl-6-oxo-1,3,4,6-tetrahydro-2H- quinolizin-3-yl]methanesulfonamide

411.3 49 rac-N-[(3S,4R)-4-({[(1s,4S)-4- (hydroxymethyl)cyclohexyl]oxy} methyl)-7-methyl-6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide

399.2

TABLE 1-8 Ex. No. IUPAC Name Structure MS 50 rac-N-[(3S,4R)-4-({[(1s,4S)-4-(3- fluorophenyl)cyclohexyl]oxy}methyl)-7-methyl-6- oxo-1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide

463.2 51 rac-N′-[(3S,4R)-4-({[(1s,4S)-4-(3- fluorophenyl)cyclohexyl]oxy}methyl)-7-methyl-6- oxo-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]-N,N- dimethylsulfuric diamide

492.2 52 rac-N-[(3S,4R)-4-({[(1s,4S)-4-(3- fluorophenyl)cyclohexyl]oxy}methyl)-7-methyl-6- oxo-1,3,4,6-tetrahydro-2H-quinolizin-3- yl]ethanesulfonamide

477.2 53 rac-N-[(3S,4R)-4-({[(1s,4S)-4-(3- fluorophenyl)cyclohexyl]oxy}methyl)-7-methyl-6- oxo-1,3,4,6-tetrahydro-2H-quinolizin-3- yl]cyclopropanesulfonamide

489.2 54 rac-N,N-dimethyl-N′-[(3S,4R)-7-methyl-6-oxo-4- ({[(1r,4S)-4-propylcyclohexyl]oxy}methyl)-1,3,4,6- tetrahydro-2H-quinolizin-3-yl]sulfuric diamide

440.3 55 N-[(3SR,4RS)-4-({[4-(difluoromethyl)cyclohexyl] oxy}methyl)-7-methyl-6-oxo-1,3,4,6-tetrahydro- 2H-quinolizin-3-yl]methanesulfonamide

419.3 56 N-[(3SR,4RS)-4-({[(1s,4S)-4-(1- methoxyethyl)cyclohexyl]oxy}methyl)-7-methyl-6- oxo-1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide

427.3

TABLE 1-9 Ex. No. IUPAC Name Structure MS 57 N-[(3SR,4RS)-4-({[4-(1- hydroxyethyl)cyclohexyl]oxy}methyl)-7-methyl-6- oxo-1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide

413.3 58 N-[(3SR,4RS)-4-({[4-(1-hydroxypropyl)cyclohexyl] oxy}methyl)-7-methyl-6-oxo-1,3,4,6-tetrahydro-2H- quinolizin-3-yl]methanesulfonamide

427.4 59 rac-N-[(3S,4R)-4-({[(1s,4S)-4- ethoxycyclohexyl]oxy}methyl)-7-methyl-6-oxo- 1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide

413.4 60 rac-N-[(3S,4R)-4-({[(1s,4S)-4-(2-hydroxypropan-2- yl)cyclohexyl]oxy}methyl)-7-methyl-6-oxo-1,3,4,6- tetrahydro-2H-quinolizin-3-yl]methanesulfonamide

427.3 61 rac-N-{(3S,4R)-7-methyl-6-oxo-4-[({(1s,4S)-4- [(propan-2-yl)oxy]cyclohexyl}oxy)methyl]-1,3,4,6- tetrahydro-2H-quinolizin-3-yl)methanesulfonamide

427.3 62 rel-N-[(3S,4R)-7-ethyl-6-oxo-4-({[(1s,4S)-4- phenylcyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro- 2H-quinolizin-3-yl]methanesulfonamide with a shorter retention time by SFC (column: CHIRALCEL OJ-3, mobile phase: carbon dioxide/0.05% diethylamine in EtOH)

459.3 63 rel-N-[(3S,4R)-7-ethyl-6-oxo-4-({[(1s,4S)-4- phenylcyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro- 2H-quinolizin-3-yl]ethanesulfonamide with a shorter retention time by SFC (column: CHIRALCEL OJ-3, mobile phase: carbon dioxide/0.05% diethylamine in EtOH)

473.3

TABLE 1-10 Ex. No. IUPAC Name Structure MS 64 rel-N-[(3S,4R)-7-ethyl-4-({[(1s,4S)-4- ethylcyclohexyl]oxy)methyl)-6-oxo-1,3,4,6- tetrahydro-2H-quinolizin-3-yl]methanesulfonamide with a longer retention time by SFC (column: CHIRALPAK IC, mobile phase: carbon dioxide/0.1% hydroxide ammonium in EtOH)

411.3 65 rel-N-[(3S,4R)-7-ethyl-4-({[(1s,4S)-4- ethylcyclohexyl]oxy}methyl)-6-oxo-1,3,4,6- tetrahydro-2H-quinolizin-3-yl]ethanesulfonamide with a longer retention time by SFC (column: CHIRALPAK AD-3, mobile phase: carbon dioxide/0.05% diethylamine in MeOH)

425.3 66 rel-N-[(3S,4R)-7-cyclopropyl-4-({[(1s,4S)-4- ethylcyclohexyl]oxy}methyl)-6-oxo-1,3,4,6- tetrahydro-2H-quinolizin-3-yl]methanesulfonamide with a shorter retention time by SFC (column: CHIRALCEL OJ-3, mobile phase: carbon dioxide/0.05% diethylamine in EtOH)

423.3 67 rel-N-[(3S,4R)-4-({[(1s,4S)-4- ethylcyclohexyl]oxy}methyl)-6-oxo-7-(propan-2-yl)- 1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide with a shorter retention time by SFC (column: CHIRALCEL OJ-3, mobile phase: carbon dioxide/0.05% diethylamine in EtOH)

425.3 68 rel-N-[(3S,4R)-7-cyclopropyl-4-({[(1s,4S)-4- ethylcyclohexyl]oxy}methyl)-6-oxo-1,3,4,6- tetrahydro-2H-quinolizin-3-yl]ethanesulfonamide with a longer retention time by SFC (column: CHIRALCEL OD-3, mobile phase: carbon dioxide/0.05% diethylamine in EtOH)

437.3 69 rel-N-[(3S,4R)-4-({[(1s,4S)-4- ethylcyclohexyl]oxy}methyl)-6-oxo-7-(propan-2-yl)- 1,3,4,6-tetrahydro-2H-quinolizin-3- yl]ethanesulfonamide with a longer retention time by SFC (column: CHIRALPAK AD-3, mobile phase: carbon dioxide/0.05% diethylamine in EtOH)

439.3 70 rel-N-[(3S,4R)-7-ethyl-4-({[(1s,4S)-4- methylcyclohexyl]oxy)methyl)-6-oxo-1,3,4,6- tetrahydro-2H-quinolizin-3-yl]methanesulfonamide with a shorter retention time by SFC (column: CHIRALCEL OJ-3, mobile phase: carbon dioxide/0.05% diethylamine in EtOH)

397.2

TABLE 1-11 Ex. No. IUPAC Name Structure MS 71 rel-N-[(3S,4R)-7-ethyl-4-(([(1s,4S)-4- methylcyclohexyl]oxy}methyl)-6-oxo-1,3,4,6- tetrahydro-2H-quinolizin-3-yl)ethanesulfonamide with a longer retentior time by SFC (column: CHIRALPAK AD-3, mobile phase: carbon dioxide/0.05% diethylamine in EtOH)

411.3 72 rel-N-{(3S,4R)-7-methyl-6-oxo-4-[({(1s,4S)-4-[2- (trifluoromethyl)phenyl]cyclohexyl}oxy)methyl]- 1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide with a shorter retention time by SFC (column: CHIRALPAK AD, mobile phase: carbon dioxide/0.1% hydroxide ammonium in 2-propanol)

513.3 73 rel-N-((3S,4R)-7-methyl-6-oxo-4-({[(1r,4S)-4- propylcyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro- 2H-quinolizin-3-yl)methanesulfonamide with a shorter retention time by SFC (column: CHIRALCEL OD-H, mobile phase: carbon dioxide/MeOH)

411.2 74 rac-N- [(3S,4R)-4-(([(1s,4S)-4-(2-methoxypropan- 2-yl)cyclohexyl]oxy) methyl)-7-methyl-6-oxo- 1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide

441.3 75 rac-N-[(3S,4R)-7-methyl-6-oxo-4-({[(1r,4S)-4- pentylcyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro- 2H-quinolizin-3-yl]methanesulfonamide

439.3 76 rac-N-[(3S,4R)-7-methyl-6-oxo-4-({[(1r,4S)-4- pentylcyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro- 2H-quinolizin-3-yl]cyclopropanesulfonamide

465.3 77 rel-N-((3S,4R)-7-methyl-6-oxo-4-({[(1r,4S)-4- propylcyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro- 2H-quinolizin-3-yl)ethanesulfonamide with a shorter retention time by SFC (column: CHIRALCEL OJ-H, mobile phase: carbon dioxide/MeOH)

425.3

TABLE 12 Ex. No. IUPAC Name Structure MS 78 rel-N-[(3S,4R)-7-methyl-6-oxo-4-({[(1r,4S)-4- propylcyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro- 2H-quinolizin-3-yl]cyclopropanesulfonamide with a shorter retention time by SFC (column: CHIRALCEL OJ-H, mobile phase: carbon dioxide/MeOH)

437.2 79 rac-N-[(3S,4R)-7-methyl-6-oxo-4-({[(1r,4S)-4- pentylcyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro- 2H-quinolizin-3-yl]ethanesulfonamide

453.3 80 rac-N-[(3S,4R)-4-({[(1r,4S)-4- butylcyclohexyl]oxy}methyl)-7-methyl-6-oxo- 1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide

425.3 81 rac-N-[(3S,4R)-4-({[(1s,4S)-4- (ethoxymethyl)cyclohexyl]oxy}methyl)-7-methyl-6- oxo-1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide

427.3 82 N-[(3SR,4RS)-7-methyl-4-(([(1s,4S)-4-{[(oxay-2- yl)oxy]methyl}cyclohexyl]oxy}methyl)-6-oxo-1,3,4,6- tetrahydro-2H-quinolizin-3-yl]methanesulfonamide

481.3 83 rac-N-[(3S,4R)-4-({[(1s,4S)-4-(2- methoxyethyl)cyclohexyl]oxy}methyl)-7-methyl-6- oxo-1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide

427.3 84 rac-ethyl [(1S,4s)-4-({(3S,4R)-3- [(methanesulfonyl)amino]-7-methyl-6-oxo-1,3,4,6- tetrahydro-2H-quinolizin-4- yl}methoxy)cyclohexyl]acetate

455.2

TABLE 1-13 Ex. No. IUPAC Name Structure MS 85 rac-N-[(3S,4R)-4-({[(1s,4S)-4-(2-hydroxy-2- methylpropyl)cyclohexyl]oxy}methyl)-7-methyl-6- oxo-1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide

441.3 86 rac-N-[(3S,4R)-4-({[(1s,4S)-4- (fluoromethyl)cyclohexyl]oxy}methyl)-7-methyl-6- oxo-1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide

401.2 87 rac-N-[(3S,4R)-4-({[(1s,4S)-4- (cyclopropylmethyl)cyclohexyl]oxy}methyl)-7- methyl-6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide

423.3 88 rac-N-[(3S,4R)-7-methyl-4-({[(1a,4S)-4-(2- methylpropyl)cyclohexyl]oxy}methyl)-6-oxo-1,3,4,6- tetrahydro-2H-quinolizin-3-yl]methanesulfonamide

425.3 89 rel-N-[(3S,4R)-4-({[(1s,4S)-4-(2,6- difluorophenyl)cyclohexyl]oxy)methyl)-7-methyl-6- oxo-1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide with a shorter retention time by SFC (column: CHIRALCEL OD-3, mobile phase: carbon dioxide/0.05% diethylamine in EtOH)

481.2 90 rel-N-[(3S,4R)-4-({[(1s,4S)-4-(2- methoxyphenyl)cyclohexyl]oxy}methyl)-7-methyl-6- oxo-1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide with a shorter retention time by SFC (column: CHIRALCEL OD-3, mobile phase: carbon dioxide/0.05% diethylamine in EtOH)

475.3 91 rel-N-[(3S,4R)-4-({[(1s,4S)-4- methylcyclohexyl]oxy}methyl)-6-oxo-7-(propan-2- yl)-1,3,4,6-tetrahydro-2H-quinolizin-3- yl]ethanesulfonamide with a longer retention time by SFC (column: CHIRALPAK AD-H, mobile phase: carbon dioxide/0.1% hydroxide ammonium in EtOH)

425.3

TABLE 1-14 Ex. No. IUPAC Name Structure MS 92 rac-N-[(3S,4R)-7-methyl-6-oxo-4-({[(1s,4S)-4- ([(propan-2-yl)oxy]methyl}cyclohexyl]oxy}methyl)- 1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide

441.2 93 rac-N-[(3S,4R)-4-({[(1s,4S)-4- cyclobutylcyclohexyl]oxy}methyl)-7-methyl-6-oxo- 1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide

423.2 94 rel-N-[(3S,4R)-7-cyclopropyl-6-oxo-4-({[(1s,4S)-4- phenylcyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro- 2H-quinolizin-3-yl]ethanesulfonamide with a shorter retention time by SFC (column: CHIRALPAK AD-3, mobile phase: carbon dioxide/0.05% diethylamine in 2-propanol)

485.3 95 rel-N-[(3S,4R)-4-({[(1s,4S)-4- methylcyclohexyl]oxy}methyl)-6-oxo-7-{propan-2- yl)-1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide with a longer retention time by SFC (column: CHIRALPAK AD-3, mobile phase: carbon dioxide/0.05% diethylamine in EtOH)

411.3 96 rel-N-[(3S,4R)-7-cyclopropyl-4-({[(1s,4S)-4- methylcyclohexyl]oxy)methyl)-6-oxo-1,3,4,6- tetrahydro-2H-quinolizin-3-yl]ethanesulfonamide with a longer retention time by SFC (column: CHIRALPAK AD-3, mobile phase: carbon dioxide/0.05% diethylamine in EtOH)

423.2 97 N-{7-methyl-4-[({(1S,4S)-4-[(1RS,2SR)-2- methylcyclopropyl]cyclohexyl)oxy)methyl]-6-oxo- 1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide

423.2 98 N-{7-methyl-4-[({(1S,4S)-4-[(1RS,2RS)-2- methylcyclopropyl]cyclohexyl)oxy)methyl]-6-oxo- 1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide

423.2

TABLE 1-15 Ex. No. IUPAC Name Structure MS 99 rac-N-[(3S,4R)-4-({[(1s,4S)-4- ethenylcyclohexyl]oxy}methyl)-7-methyl-6-oxo- 1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide

395.3 100 rel-N-[(3S,4R)-6-oxo-4-({[(1s,4S)-4- phenylcyclohexyl]oxy}methyl)-7-(propan-2-yl)- 1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide with a shorter retention time by SFC (column: CHIRALCEL OJ-3, mobile phase: carbon dioxide/0.05% diethylamine in EtOH)

473.3 101 rel-N-[(3S,4R)-7-cyclopropyl-6-oxo-4-({[(1s,4S)-4- phenylcyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro- 2H-quinolizin-3-yl]methanesulfonamide with a shorter retention time by SFC (column: CHIRALPAK AD-3, mobile phase: carbon dioxide/0.05% diethylamine in 2-propanol)

471.2 102 rel-N-[(3S,4R)-6-oxo-4-({[(1s,4S)-4- phenylcyclohexyl]oxy}methyl)-7-(propan-2-yl)- 1,3,4,6-tetrahydro-2H-quinolizin-3- yl]ethanesulfonamide with a shorter retention time by SFC (column: CHIRALCEL OJ-3, mobile phase: carbon dioxide/0.05% diethylamine in EtOH)

487.2 103 N-{7-methyl-4-[({(1S,4S)-4-[(1RS,2SR)-2- methylcyclopropyl)cyclohexyl)oxy)methyl]-6-oxo- 1,3,4,6-tetrahydro-2H-quinolizin-3- yl]ethanesulfonamide

437.3 104 N-{7-methyl-4-[({(1S,4S)-4-[(1RS,2SR)-2- methylcyclopropyl]cyclohexyl)oxy)methyl]-6-oxo- 1,3,4,6-tetrahydro-2H-quinolizin-3- yl]cyclopropanesulfonamide

449.2 105 rel-N-[(3S,4R)-7-cyclopropyl-4-({[(1s,4S)-4- methylcyclohexyl]oxy)methyl)-6-oxo-1,3,4,6- tetrahydro-2H-quinolizin-3-yl]methanesulfonamide with a shorter retention time by SFC (column: CHIRALCEL OJ-3, mobile phase: carbon dioxide/0.05% diethylamine in EtOH)

409.3

TABLE 1-16 Ex. No. IUPAC Name Structure MS 106 rel-N-[(3S,4R)-9-bromo-4-({[(1s,4S)-4- ethylcyclohexyl]oxy}methyl)-7-methyl-6-oxo- 1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide with a shorter retention time by SFC (column: CHIRALCEL OD-3, mobile phase: carbon dioxide/0.05% diethylamine in EtOH)

475.1 107 rel-N-[(3S,4R)-7-methyl-6-oxo-4-({[(1s,4S)-4- propoxycyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro- 2H-quinolizin-3-yl]methanesulfonamide with a shorter retention time by SFC (column: CHIRALCEL OJ-3, mobile phase: carbon dioxide/0.05% diethylamine in EtOH}

427.3 108 rac-N-[(3S,4R)-4-({[(1s,4S)-4- ethynylcyclohexyl]oxy}methyl)-7-methyl-6-oxo- 1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide

393.2 109 rac-N-[(3S,4R)-7-methyl-6-oxo-4-({[(1s,4S)-4- (prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-1,3,4,6- tetrahydro-2H-quinolizin-3-yl]methanesulfonamide

407.3 110 rac-N-{(3S,4R)-7-methyl-6-oxo-4-[({(1s,4S)-4- [(1Z)-prop-1-en-1-yl]cyclohexyl)oxy)methyl]- 1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide

409.3 111 rel-N-[(3S,4R)-4-({[(1s,4S)-4- cyclopropylcyclohexyl]oxy}methyl)-7-methyl-6-oxo- 1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide with a shorter retention time by HPLC (column: CHIRALCEL OJ-H, mobile phase: hexane/EtOH)

409.3 112 rel-N-[(3S,4R)-4-({[(1s,4S)-4- cyclopropylcyclohexyl]oxy}methyl)-7-methyl-6-oxo- 1,3,4,6-tetrahydro-2H-quinolizin-3- yl]ethanesulfonamide with a shorter retention time by HPLC (column: CHIRALCEL OJ-H, mobile phase: hexane/EtOH)

423.3

TABLE 1-17 Ex. No. IUPAC Name Structure MS 113 rel-N-[(3S,4R)-4-({[(1s,4S)-4- cyclopropylcyclohexyl]oxy)methyl)-7-methyl-6-oxo- 1,3,4,6-tetrahydro-2H-quinolizin-3- yl]cyclopropanesulfonamide with a shorter retention time by HPLC (column: CHIRALCEL OJ-H, mobile phase: hexane/EtOH)

435.3 114 rel-N-[(3S,4R)-4-(([(1s,4S)-4- (cyclopropylmethoxy)cyclohexyl]oxy}methyl)-7- methyl-6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide with a shorter retention time by SFC (column: CHIRALCEL OJ-3, mobile phase: carbon dioxide/0.05% diethylamine in EtOH)

439.3 115 rel-N-[(3S,4R)-9-cyclopropyl-4-({[(1s,4S)-4- ethylcyclohexyl]oxy) methyl)-7-methyl-6-oxo- 1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide with a shorter retention time by SFC (column: CHIRALCEL OJ-3, mobile phase: carbon dioxide/0.05% diethylamine in EtOH)

437.3 116 rac-N-[(3S,4R)-7-methyl-6-oxo-4-({[(1s,4S)-4- (pyridin-2-yl)cyclohexyl]oxy)methyl)-1,3,4,6- tetrahydro-2H-quinolizin-3-yl]methanesulfonamide

446.2 117 rac-N-[(3S,4R)-4-({[(1s,4S)-4- ethylcyclohexyl]oxy}methyl)-9-fluoro-7-methyl-6- oxo-1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide

415.1 118 rac-N-[(3S,4R)-9-cyano-4-({[(1s,4S)-4- ethylcyclohexyl]oxy}methyl)-7-methyl-6-oxo- 1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide

422.2 119 rac-N-[(3S,4R)-4-({[(1s,4S)-4- ethylcyclohexyl]oxy)methyl)-6-oxo-1,3,4,6- tetrahydro-2H-quinolizin-3-yl]methanesulfonamide

383.1 120 rac-N-[(3S,4R)-7-bromo-4-({[(1s,4S)-4- ethylcyclohexyl]oxy)methyl)-6-oxo-1,3,4,6- tetrahydro-2H-quinolizin-3-yl]methanesulfonamide

462.8

TABLE 1-18 Ex. No. IUPAC Name Structure MS 121 rac-N-[(3S,4R)-7-methyl-6-oxo-4-({[(1s,4S)-4- (prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-1,3,4,6- tetrahydro-2H-quinolizin-3-yl]ethanesulfonamide

421.3 122 rac-N-[(3S,4R)-7-methyl-6-oxo-4-({[(1s,4S)-4- (prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-1,3,4,6- tetrahydro-2H-quinolizin-3- yl]cyclopropanesulfonamide

433.4 123 rac-N-[(3S,4R)-4-({[(1s,4S)-4-(but-1-yn-1- yl)cyclohexyl]oxy}methyl)-7-methyl-6-oxo-1,3,4,6- tetrahydro-2H-quinolizin-3-yl]methanesulfonamide

421.0 124 rac-N-[(3S,4R)-7-methyl-4-({[(1s,4S)-4- (3- methylbut-1-yn-1-yl)cyclohexyl]oxy}methyl)-6-oxo- 1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide

435.3 125 rac-N-[(3S,4R)-4-({[(1s,4S)-4- (cyclopropylethynyl)cyclohexyl]oxy}methyl)-7- methyl-6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide

433.2 126 rac-N-[(3S,4R)-7-methyl-6-oxo-4-({[(1s,4S)-4- (pent-1-yn-1-yl)cyclohexyl]oxy}methyl)-1,3,4,6- tetrahydro-2H-quinolizin-3-yl]methanesulfonamide

435.1 127 rac-N-{(3S,4R)-7-methyl-6-oxo-4-[({(1s,4S)-4- [(1Z)-prop-1-en-1-yl]cyclohexyl}oxy)methyl]- 1,3,4,6-tetrahydro-2H-quinolizin-3- yl}ethanesulfonamide

423.4 128 rac-N-{(3S,4R)-7-methyl-6-oxo-4-[({(1s,4S)-4- [(1Z)-prop-1-en-1-yl]cyclohexyl}oxy)methyl]- 1,3,4,6-tetrahydro-2H-quinolizin-3- yl]cyclopropanesulfonamide

435.4 129 rac-N-{(3S,4R)-7-methyl-6-oxo-4-[({(1s,4S)-4- [(1E)-prop-1-en-1-yl]cyclohexyl}oxy)methyl]- 1,3,4,6-tetrahydro-2H-quinolizin-3- yl}methanesulfonamide

409.1 130 rac-N-{(3S,4R)-4-[({(1s,4S)-4-[(1Z)-but-1-en-1- yl]cyclohexyl}oxy)methyl]-7-methyl-6-oxo-1,3,4,6- tetrahydro-2H-quinolizin-3-yl}methanesulfonamide

423.2

TABLE 19 Ex. No. IUPAC Name Structure MS 131 rac-N-[(3S,4R)-4-({[(1s,4S)-4- ethoxycyclohexyl]oxy}methyl)-7-methyl-6-oxo- l,3,4,6-tetrahydro-2H-quinolizin-3- yl]ethanesulfonamide

427.0 132 rac-N-[(3S,4R)-4-({[(1s,4S)-4- ethoxycyclohexyl]oxy}methyl)-7-methyl-6-oxo- 1,3,4,6-tetrahydro-2H-quinolizin-3- yl]cyclopropanesulfonamide

439.1 133 rac-N-[(3S,4R)-4-({[(1s,4S)-4- (cyclobutyloxy)cyclohexyl]oxy}methyl)-7-methyl-6- oxo-1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide

439.1 134 rac-N-[(3S,4R)-4-({[(1s,4S)-4- ethylcyclohexyl]oxy}methyl)-9-methoxy-7-methyl- 6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide

427.1 135 rac-N-[(3S,4R)-4-({[(1s,4S)-4- ethylcyclohexyl]oxy}methyl)-7-fluoro-6-oxo-1,3,4,6- tetrahydro-2H-quinolizin-3-yl]methanesulfonamide

401.3 136 rac-N-[(3S,4R)-4-({[(1s,4S)-4- ethylcyclohexyl]oxy}methyl)-7-(hydroxymethyl)-6- oxo-1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide

413.3 137 rac-N-[(3S,4R)-4-({[(1s,4S)-4- ethylcyclohexyl]oxy}methyl)-7-(2-hydroxypropan-2- yl)-6-oxo-1,3,4t6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide

423.1 138 rac-N-[(3S,4R)-4-({[(1s,4S)-4- ethylcyclohexyl] oxy}methyl)-7-methoxy-6-oxo- 1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide

413.3 139 rac-N-[(3S,4R)-7-cyano-4-({[(1s,4S)-4- ethylcyclohexyl]oxy}methyl)-6-oxo-1,3,4,6- tetrahydro-2H-quinolizin-3-yl]methanesulfonamide

408.3 140 rac-N-[(3S,4R)-4-({[(1s,4S)-4-(furan-2- yl)cyclohexyl]oxy}methyl)-7-methyl-6-oxo-1,3,4,6- tetrahydro-2H-quinolizin-3-yl]methanesulfonamide

435.3

TABLE 1-20 Ex. No. IUPAC Name Structure MS 141 rac-N-[(3S,4R)-7-methyl-4-({[(1s,4S)-4-(1-methyl- 1H-pyrazol-3-yl)cyclohexyl]oxy}methyl)-6-oxo- 1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide

449.1 142 rac-N-[(3S,4R)-7-methyl-4-({[(1s,4S)-4-(1-methyl- 1H-pyrazol-4-yl)cyclohexyl]oxy}methyl)-6-oxo- 1,3,4,6-tetrahydro-2H-quinolizin-3- yl]methanesulfonamide

449.3 143 N-[(3SR,4RS)-7-methyl-4-({[(1s,4S)-4-(oxetan-2- yl)cyclohexyl]oxy}methyl)-6-oxo-1,3,4,6-tetrahydro- 2H-quinolizin-3-yl]methanesulfonamide

425.2

Ex. No. IUPAC Name Structure MS 144 rel-N,N-dimethyl-N′-[(3S,4R)-7-methyl-6-oxo- 4-({[(1s,4S)-4-(prop-1-yn-1-yl)cyclohexyl]oxy} methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3- yl]sulfuric diamide with a shorter retention time by SFC (column: CHIRALPAK AS-3, mobile phase: carbon dioxide/0.05% diethylamine in EtOH)

436.4 145 rel-N-[(3S,4R)-4-({[(1s,4S)-4-ethoxycyclohexyl] oxy}methyl)-7-methyl-6-oxo-1,3,4,6-tetrahydro- 2H-quinolizin-3-yl]methanesulfonamide with a longer retention time by SFC (column: CHIRALPAK AD-3, mobile phase: carbon dioxide/0.05% diethylamine in EtOH)

413.4 146 rel-N-[(3S,4R)-7-methyl-6-oxo-4-({[(1s,4S)-4- (prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-1,3,4,6- tetrahyd ro-2H-quinolizin-3-yl]methanesulfonamide with a longer retention time by SFC (column: CHIRALPAK AD-3, mobile phase: carbon dioxide/ 0.05% diethylamine in EtOH)

407.4 147 rel-N-[(3S,4R)-7-methyl-6-oxo-4-({[(1s,4S)-4- (prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-1,3,4,6- tetrahydro-2H-quinolizin-3-yl]ethanesulfonamide with a shorter retention time by SFC (column: CHIRALCEL OJ-3, mobile phase: carbon dioxide/ 0.05% diethylamine in EtOH)

421.4 148 rel-N-[(3S,4R)-7-methyl-6-oxo-4-({[(1s,4S)-4- (prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-1,3,4,6- tetrahydro-2H-quinolizin-3-yl] cyclopropanesulfonamide with a longer retention time by SFC (column: CHIRALPAK AD-3, mobile phase: carbon dioxide/0.05% diethylamine in EtOH)

433.4 149 rel-N-methyl-N′-[(3S,4R)-7-methyl-6-oxo-4-({[(1s, 4S)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)- 1,3,4,6-tetrahydro-2H-quinolizin-3-yl] sulfuric diamide with a longer retention time by HPLC (column: CHIRALPAK IC, mobile phase: hexane/ EtOH)

422.4

Experimental Example 1: Obtainment of Cell Stably Expressing Human Orexin Type 2 Receptor (hOX2R)

To obtain a cell clone stably expressing human orexin type 2 receptor, human orexin type 2 receptor cDNA was inserted into pcDNA3.1(+) plasmid vector (Invitrogen), and a plasmid DNA for expression of human orexin type 2 receptor (pcDNA3.1(+)/hOX2R) was cloned. The plasmid DNA was introduced into CHO-K1 cell by an electroporation method, and human orexin type 2 receptor-expressing clonal cells were obtained by limiting dilution method using G418 drug resistance as a selection marker.

Experimental Example 2: Measurement of Orexin Type 2 Receptor Agonist Activity

CHO cells forcibly expressing human OX2 receptor were seeded in each well of 384 well black transparent bottom plate (BD Falcon) at 10,000 cells/well, and cultured for one day in a 5% CO₂ incubator at 37° C. After removal of the medium in the cell plate, assay buffer A containing a calcium indicator (HBSS (Thermo Fisher Scientific), 20 mM HEPES (Thermo Fisher Scientific), 0.1% BSA (Sigma-Aldrich), 2.5 μg/mL Fluo-4 AM (DOJINDO Chemical), 0.08% Pluronic F127 (DOJINDO Chemical), 1.25 mM probenecid (DOJINDO Chemical)) was added thereto at 30 μL/well. The plate was stood for 30 min in a 5% CO₂ incubator at 37° C., and further stood at room temperature for 30 min. A test compound prepared by diluting with assay buffer B (HBSS, 20 mM HEPES, 0.1% BSA) was added thereto at 10 μL/well, and the fluorescence value was measured by FDSSμCELL (Hamamatsu Photonics K.K.) every one sec for 1 min, and thereafter every two sec for 1 min 40 sec. The activity (%) of the test compound was calculated assuming that variation in the fluorescence value when DMSO was added instead of the test compound was 0%, and variation in the fluorescence value when orexin A (human) (PEPTIDE INSTITUTE, INC.) was added at the final concentration of 10 nM was 100%. The activity of each compound at the concentration of 3 μM was shown in Table 2. As is clear from the results, the compound of the present invention was shown to have an agonist activity on human orexin type 2 receptor.

TABLE 2 OX2R agonist Ex.No. activity (3 μM, %) 1 96 2 94 3 89 4 58 5 112 6 92 7 85 8 80 9 68 10 97 11 100 12 96 13 96 14 100 15 96 16 91 17 86 18 96 19 89 20 97 21 92 22 83 23 96 24 86 25 92 26 86 27 85 28 92 29 83 30 94 31 89 32 81 33 78 34 78 35 88 36 84 37 94 38 88 40 89 45 95 48 95 55 88 56 84 58 95 59 93 61 103 66 96 71 99 72 108 74 95 76 93 78 88 80 99 81 102 82 56 83 91 84 75 85 59 86 89 87 89 88 95 89 89 90 87 92 90 93 97 97 94 99 91 105 84 106 91 107 65 108 101 109 96 110 91 111 98 112 94 113 87 114 69 115 100 116 99 144 99 145 96 146 104 147 99 148 97 149 95

Experimental Example 3: Evaluation of Wake-Promoting Effects in SD Rats

The wake-promoting effects were evaluated by measuring the electroencephalogram (EEG) and electromyogram (EMG) in SD rats (Crl:CD(SD), CHARLES RIVER LABORATORIES JAPAN, Kanagawa, Japan.) using chronic experiment telemetry automatic measurement system (PhysioTel® and PONEMAH™, Data Sciences International Inc., Minnesota, U.S.A.). Under pentobarbital sodium anesthesia (50 mg/kg, intraperitoneal, Somnopentyl®, Kyoritsu Seiyaku, Tokyo, Japan), 8-old male SD rats were surgically implanted with a transmitter. Bilateral EEG leads were positioned at the parietal area in contact with the dura and fixed to the cranium with dental cement. Bilateral EMG leads were fixed to the back cervical muscles. The transmitter was implanted under the skin of the back, and the head skin was sutured. After recovery for at least 1 week, the cortical EEG and EMG signals in SD rats were recorded using a high-end data acquisition and real-time analysis system (PONEMAH™). The signals were automatically scored in 4 sec epochs by a sleep scoring system (SleepSign®, Kissei Comtec Co., Ltd.). Test compound (1 or 10 mg/kg, Table 3) dissolved in a mixed solution containing 10% DMSO, 10% Cremophor EL, 20% PEG400 and 60% H₂O, or vehicle (i.e., a mixed solution containing 10% DMSO, 10% Cremophor EL, 20% PEG400 and 60% H₂O) was administered subcutaneously (s.c.) to the SD rats at zeitgeber time (ZT) 5 (when the start time of the light cycle was ZTO) in a volume of 5 mL/kg. The EEG and EMG recordings were performed for 3 hr after the compound administration. The time spent in wakefulness for 3 hr after administration (% of vehicle treatment) was calculated by using SleepSign. The results are shown in Table 3.

TABLE 3 Test Wakefulness time compound (% of vehicle treatment) (s.c.) Dose (mg/kg) (Mean, n = 2) Example 78 1 130.53 Example 111 1 126.37 Example 146 10 166.75

As is clear from the results, the test compounds of the present invention increased the wakefulness time as compared with the vehicle treatment group in SD rats.

Experimental Example 4: Evaluation of Wake-Promoting Effects in Cynomolgus Monkeys

The wake-promoting effects were evaluated by measuring the electroencephalogram (EEG), electromyogram (EMG) and electrooculogram (EOG) in cynomolgus monkeys. Under isoflurane anesthesia (1-5%, Pfizer Japan Inc., Tokyo, Japan), male cynomolgus monkeys (2-3 years old, Hamri Co., Ltd., Ibaraki, Japan) were surgically implanted with radio-telemetry transmitters (TL10M3-D70-EEE, Data Sciences International Inc., MN, USA). EEG leads were stereotaxically positioned at the parietal area and secured to the cranium with stainless-steel screws in contact with the dura. Unilateral EOG leads were positioned at superior orbital margin of one eye and secured with stainless-steel screws. Bilateral EMG leads were implanted into the back cervical muscles. After the surgery, each monkey was given penicillin (100,000 units/head, i.m., Meiji Seika Pharma Co., Ltd., Tokyo, Japan), buprenorphine (0.02 mg/kg, i.m., Otsuka Pharmaceutical Co., Ltd., Tokyo, Japan) and prednisolone (1 mg/kg, s.c., Kyoritsu Seiyaku Co., Ltd., Tokyo, Japan) daily for one week. After at least a 1-month recovery period in home cages, the monkeys were habituated to the recording chamber (an acrylic cage 60W×55D×75H (cm)) located in a soundproof, electrically shielded room. After confirming that the monkeys slept sufficiently in the experimental room, the cortical EEG, EMG and EOG signals were recorded using Dataquest ART software (Data Sciences International Inc., MN, USA). The signals were semi automatically scored in 20 sec epochs by a sleep scoring system (SleepSign, Kissei Comtec Co., Ltd., Nagano, Japan). This preliminary scoring was visually inspected and corrected if necessary. Test compound (0.1, 0.3, 1 or 10 mg/kg, Table 4) dissolved in a mixed solution containing 5% DMSO, 5% Cremophor EL, 20% PEG400 and 70% soluplus (1% (w/v)), or vehicle (i.e., a mixed solution containing 5% DMSO, 5% Cremophor EL, 20% PEG400 and 70% soluplus (1% (w/v))) was administered subcutaneously (s.c.) to the cynomolgus monkeys at ZT12 in a volume of 0.5 mL/kg in a pre-post design. The EEG, EMG and EOG recordings were performed for 4 h after the compound administration. The time spent in wakefulness for 4 h after administration (% of vehicle treatment) was calculated by using SleepSign. The results are shown in Table 4.

TABLE 4 Test Wakefulness time compound Dose (% of vehicle treatment) (s.c.) (mg/kg) (Mean, n = 2) Example 6 0.3 352.87 Example 9 0.3 521.43 Example 112 0.1 671.65 Example 113 0.1 380.66 Example 145 10 277.67 Example 147 1 389.64 Example 148 1 324.87

As is clear from the results, the test compounds of the present invention increased the wakefulness time as compared with the vehicle treatment group in cynomolgus monkeys. That is, these compounds were shown to be effective for the treatment of narcolepsy.

Formulation Example 1 (Production of Capsule)

1) compound of Example 1 30 mg 2) crystalline cellulose 10 mg 3) lactose 19 mg 4) magnesium stearate 1 mg total 60 mg

1), 2), 3) and 4) are mixed and filled in a gelatin capsule.

Formulation Example 2 (Production of Tablet)

1) compound of Example 1  30 g 2) lactose  50 g 3) cornstarch  15 g 4) calcium carboxymethylcellulose  44 g 5) magnesium stearate   1 g 1000 tablets 140 g in total

The total amount of 1), 2), 3) and 30 g of 4) are kneaded with water, vacuum dried and sieved. The sieved powder is mixed with 14 g of 4) and 1 g of 5), and the mixture is punched by a tableting machine. In this way, 1000 tablets containing 30 mg of the compound of Example 1 per tablet are obtained.

INDUSTRIAL APPLICABILITY

The compound of the present invention has an orexin type 2 receptor agonist activity, and is useful as an agent for the prophylaxis or treatment of narcolepsy.

This application is based on patent application No. 2019-214206 filed on Nov. 27, 2019 and patent application No. 2020-093604 filed on May 28, 2020 in Japan, the contents of which are encompassed in full herein. 

1. A compound represented by the formula:

wherein R¹ is an optionally substituted C₁₋₆ alkyl group, an optionally substituted mono- or di-C₁₋₆ alkylamino group, or an optionally substituted C₃₋₆ cycloalkyl group; R² is a hydrogen atom, a halogen atom, a cyano group, an optionally substituted C₁₋₆ alkyl group, an optionally substituted C₃₋₆ cycloalkyl group, or an optionally substituted C₁₋₆ alkoxy group; R³ is a hydrogen atom, a halogen atom, a cyano group, an optionally substituted C₁₋₆ alkyl group, an optionally substituted C₃₋₆ cycloalkyl group, or an optionally substituted C₁₋₆ alkoxy group; and R⁴ is an optionally substituted C₁₋₆ alkyl group, an optionally substituted C₃₋₆ cycloalkyl group, an optionally substituted C₂₋₆ alkenyl group, an optionally substituted C₂₋₆ alkynyl group, an optionally substituted C₆₋₁₀ aryl group, an optionally substituted C₁₋₆ alkoxy group, an optionally substituted C₃₋₆ cycloalkoxy group, or an optionally substituted 4- to 6-membered heterocyclic group, or a salt thereof.
 2. The compound or salt according to claim 1, wherein R² is a hydrogen atom, a halogen atom, an optionally substituted C₁₋₆ alkyl group, or an optionally substituted C₁₋₆ alkoxy group; R³ is a hydrogen atom, a halogen atom, an optionally substituted C₁₋₆ alkyl group, an optionally substituted C₃₋₆ cycloalkyl group, or an optionally substituted C₁₋₆ alkoxy group; and R⁴ is an optionally substituted C₁₋₆ alkyl group, an optionally substituted C₆₋₁₀ aryl group, or an optionally substituted C₁₋₆ alkoxy group.
 3. The compound or salt according to claim 1, wherein R¹ is a C₁₋₆ alkyl group, a mono- or di-C₁₋₆ alkylamino group, or a C₃₋₆ cycloalkyl group; R² is a hydrogen atom, a halogen atom, a cyano group, a C₁₋₆ alkyl group, a C₃₋₆ cycloalkyl group, or a C₁₋₆ alkoxy group; R³ is a hydrogen atom, a halogen atom, a cyano group, a C₁₋₆ alkyl group optionally substituted by 1 to 3 hydroxy groups, a C₃₋₆ cycloalkyl group, or a C₁₋₆ alkoxy group; and R⁴ is (1) a C₁₋₆ alkyl group optionally substituted by 1 to 3 substituents selected from a halogen atom, a hydroxy group, a C₃₋₆ cycloalkyl group, a C₁₋₆ alkoxy group, a C₁₋₆ alkoxy-carbonyl group, and a 3- to 14-membered non-aromatic heterocyclyloxy group, (2) a C₃₋₆ cycloalkyl group optionally substituted by 1 to 3 C₁₋₆ alkyl groups, (3) a C₂₋₆ alkenyl group, (4) a C₂₋₆ alkynyl group optionally substituted by 1 to 3 C₃₋₆ cycloalkyl groups, (5) a C₆₋₁₀ aryl group optionally substituted by 1 to 3 substituents selected from a halogen atom, an optionally halogenated C₁₋₆ alkyl group, and a C₁₋₆ alkoxy group, (6) a C₁₋₆ alkoxy group optionally substituted by 1 to 3 C₃₋₆ cycloalkoxy groups, (7) a C₃₋₆ cycloalkoxy group, or (8) a 4- to 6-membered heterocyclic group optionally substituted by 1 to 3 C₁₋₆ alkyl groups.
 4. The compound or salt according to claim 1, wherein R¹ is a C₁₋₆ alkyl group, a mono- or di-C₁₋₆ alkylamino group, or a C₃₋₆ cycloalkyl group; R² is a hydrogen atom; R³ is a C₁₋₆ alkyl group; and R⁴ is (1) a C₁₋₆ alkyl group, (2) a C₃₋₆ cycloalkyl group, (3) a C₂₋₆ alkenyl group, (4) a C₂₋₆ alkynyl group, (5) a C₆₋₁₀ aryl group, or (6) a C₁₋₆ alkoxy group.
 5. The compound or salt according to claim 1, wherein R¹ is a C₁₋₆ alkyl group; R² is a hydrogen atom; R³ is a C₁₋₆ alkyl group; and R⁴ is (1) a C₁₋₆ alkyl group, (2) a C₂₋₆ alkynyl group, or (3) a C₁₋₆ alkoxy group.
 6. N-[4-({[(1s,4S)-4-Ethoxycyclohexyl]oxy}methyl)-7-methyl-6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]methanesulfonamide or a salt thereof.
 7. N-[7-Methyl-6-oxo-4-({[(1s,4S)-4-(prop-1-yn-1-yl)cyclohexyl]oxy}methyl)-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]ethanesulfonamide or a salt thereof.
 8. N-[4-({[(1s,4S)-4-Ethylcyclohexyl]oxy}methyl)-7-methyl-6-oxo-1,3,4,6-tetrahydro-2H-quinolizin-3-yl]methanesulfonamide or a salt thereof.
 9. A medicament comprising the compound or salt according to claim
 1. 10. The medicament according to claim 9, which is an orexin type 2 receptor agonist.
 11. The medicament according to claim 9, which is an agent for the prophylaxis or treatment of narcolepsy.
 12. The compound or salt according to claim 1 for use in the prophylaxis or treatment of narcolepsy.
 13. A method for activating an orexin type 2 receptor in a mammal, which comprises administering an effective amount of the compound or salt according to claim 1 to the mammal.
 14. A method for preventing or treating narcolepsy in a mammal, which comprises administering an effective amount of the compound or salt according to claim 1 to the mammal.
 15. Use of the compound or salt according to claim 1 for the manufacture of an agent for the prophylaxis or treatment of narcolepsy. 